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Substance and method for detecting tortoise-shell glue, deer-horn glue and products thereof

A technology of deer horn glue and tortoise shell glue, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of high content

Active Publication Date: 2014-03-12
SHAN DONG DONG E E JIAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there is no detection method for one-time identification of counterfeit components in tortoise shell glue, deer antler glue and their products from the perspective of collagen peptides

Method used

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  • Substance and method for detecting tortoise-shell glue, deer-horn glue and products thereof
  • Substance and method for detecting tortoise-shell glue, deer-horn glue and products thereof
  • Substance and method for detecting tortoise-shell glue, deer-horn glue and products thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0035] (1) Preparation of gel sample enzymatic hydrolysis solution

[0036] Take 1.0g of the gel sample to be tested in a 500mL measuring bottle, add a small amount of 1%NH 4 HCO 3 solution, sonicate to dissolve the sample completely, and use 1% NH 4 HCO 3 Dilute the solution (pH 8.0) to the mark, shake well, filter through a microporous membrane, accurately measure 1 mL of the subsequent filtrate plus 100 μL of 2 mg / mL trypsin solution, and enzymatically hydrolyze at a constant temperature of 37 °C for 6 h.

[0037] (2) Preparation of enzymatic hydrolysis solution of synthetic polypeptide control sample

[0038] Weigh a certain amount of synthetic peptide, add 1% NH 4 HCO 3 Solution (pH8.0), shake well, prepare to a concentration of about 0.3μg / mL, filter through a microporous membrane, and set aside.

[0039] 3 Discovery of peptides

[0040] (1) Discovery of ions

[0041] Take 5 μL of the enzymatic hydrolysis solution and put it into the liquid mass spectrometer for ...

Embodiment 1

[0050] 1 Materials and Reagents

[0051] Materials: Pure glue includes donkey-hide gelatin, horsehide glue, yellow gelatin, pigskin glue, tortoise shell glue, antler glue, and sheepskin glue, which are obtained by decocting donkey hide, horse hide, cowhide, pig skin, tortoise shell, antler, and sheepskin respectively.

[0052] Mixed glue samples (tortoiseshell glue containing 5% yellow gelatin, 10% yellow gelatin tortoiseshell glue, 20% yellow gelatin tortoiseshell glue, 40% yellow gelatin tortoiseshell glue) are made by accurately mixing the above pure glue (percentage is mass Fraction).

[0053] Reagents: ammonium bicarbonate (analytical pure), trypsin (sequentially pure, purchased from China Institute for Food and Drug Control), synthetic polypeptide Gly-Val-Val-Gly-Leu-Pro-Gly-Gln-Arg.

[0054] 2 detection method

[0055] (1) Preparation of synthetic peptide control sample enzymatic hydrolysis solution

[0056] Take 1.0g sample of pure tortoise shell glue in a 500ml mea...

Embodiment 2

[0063] 1 Materials and Reagents

[0064] Materials: The mixed glue sample is made by adding 5% fake glue to the pure deer antler glue in Example 1, including deer antler glue containing 5% donkey-hide gelatin, 5% horsehide glue, and antlers containing 5% yellow gelatin Glue, antler glue with 5% pigskin glue, and antler glue with 5% sheepskin glue (percentages are mass fractions).

[0065] Reagents: ammonium bicarbonate (analytical pure), trypsin (sequentially pure, purchased from China Institute for Food and Drug Control), synthetic polypeptide Gly-Val-Val-Gly-Leu-Pro-Gly-Gln-Arg.

[0066] 2 detection method

[0067] (1) Preparation of synthetic peptide control sample enzymatic hydrolysis solution

[0068] Take 1.0g sample of pure antler gum to be tested in a 500ml measuring bottle, add a small amount of 1%NH 4 HCO 3 solution, sonicate to dissolve the sample completely, and use 1% NH 4 HCO 3 Dilute the solution (pH 8.0) to the mark, shake well, filter through a microporo...

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PUM

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Abstract

The invention provides a method for identifying tortoise-shell glue, deer-horn glue and products thereof. The method provided by the invention comprises the step of detecting by using the difference between the specific nucleotide sequence or amino acid sequence of genomes of the tortoise-shell glue, deer-horn glue and products thereof and the specific nucleotide sequence or amino acid sequence of genomes of other imitations, wherein the specific amino acid sequence is shown in SEQ. ID No1. According to the method provided by the invention, fake ingredients, such as donkeys, horses, cattle, pigs or sheep, in the tortoise-shell glue, deer-horn glue and products thereof can be detected rapidly, so as to distinguish the authenticity. The invention further relates a polypeptide and a composition formed by the polypeptide and a corresponding carrier. Furthermore, the invention relates to application of the polypeptide and composition thereof in detection of donkey, horse, cattle, pig or sheep-derived ingredients in the tortoise-shell glue and products thereof.

Description

technical field [0001] The invention relates to a method for detecting ingredients derived from donkeys, horses, cattle, pigs or sheep, which can be specifically applied to authenticity identification of tortoise shell glue, deer horn glue and products thereof, and belongs to the field of traditional Chinese medicine detection. Background technique [0002] According to the 2010 edition of the Chinese Pharmacopoeia, tortoise shell jelly is a solid gel made from tortoise shells, decocted, and concentrated. It is salty, sweet, and cool in nature, and belongs to the liver, kidney, and heart channels. It can nourish yin, nourish blood, and stop bleeding. It is mainly used for hot flashes due to yin deficiency, night sweats caused by bone steaming, soreness of the waist and knees, blood deficiency and chlorosis, metrorrhagia and vaginal discharge, etc. Long-term consumption of tortoise shell glue is also beneficial to enhance the body's self-regulation, prolong life, and also ben...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
Inventor 周祥山秦玉峰尤金花田守生嵇传良郭尚伟段小波史兆松
Owner SHAN DONG DONG E E JIAO
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