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Reagent card for colloidal gold immunochromatograohic assay of lily mottle virus and preparation method of reagent card for colloidal gold immunochromatograohic assay of lily mottle virus

A lily mottle virus, immunochromatographic detection technology, applied in the direction of measuring devices, analysis materials, biological material analysis, etc., can solve the problem that it is difficult to meet the needs of virus detection, and achieve rapid detection, high detection accuracy, and high detection sensitivity Effect

Active Publication Date: 2014-10-29
NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no relevant reports on the detection of LMoV by colloidal gold immunochromatography at home and abroad, and there is no commercialized LMoV colloidal gold immunochromatography detection reagent card, which is difficult to meet the virus detection needs of domestic ornamental and edible lilies.

Method used

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  • Reagent card for colloidal gold immunochromatograohic assay of lily mottle virus and preparation method of reagent card for colloidal gold immunochromatograohic assay of lily mottle virus
  • Reagent card for colloidal gold immunochromatograohic assay of lily mottle virus and preparation method of reagent card for colloidal gold immunochromatograohic assay of lily mottle virus

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Embodiment Construction

[0020] Such as figure 1 and figure 2The shown LMoV colloidal gold immunochromatography detection reagent card includes a reagent card slot 1, a backing plate 10, a sample pad 6, a colloidal gold binding pad 7, a nitrocellulose membrane 8, and a water-absorbing filter paper 9, wherein the reagent card slot 1 includes an upper The shell and the lower shell, the upper shell and the lower shell are connected by buckles, the upper shell is provided with a first hole 2 and a second hole 3, the sample pad 6 is placed under the first hole 2, and the nitrocellulose membrane 8 Placed below the second hole 3, the colloidal gold binding pad 7 contains a gold standard probe, the liner 10 is fixed in the reagent card slot 1, the sample pad 6, the colloidal gold binding pad 7, the nitrocellulose membrane 8 and the absorbent filter paper 9 Arranged in order and connected to the upper surface of the liner 10, the nitrocellulose membrane 8 is provided with a detection line 4 and a control lin...

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Abstract

The invention discloses a reagent card for colloidal gold immunochromatograohic assay of lily mottle virus and a preparation method of the reagent card for the colloidal gold immunochromatograohic assay of the lily mottle virus. The reagent card comprises a reagent card groove, a lining plate, a sample pad, a colloidal-gold combined pad, a nitrocellulose membrane and water-absorbing filtering paper, wherein the colloidal-gold combined pad contains a gold labeling probe; the lining plate is fixed in the reagent card groove; the sample pad, the colloidal-gold combined pad, the nitrocellulose membrane and the water-absorbing filtering paper are arranged connected to the upper surface of the lining plate in sequence; the nitrocellulose membrane is provided with a detection line and a control line which are respectively coated with rabbit-anti-LMoV polyclonal antibodies and goat-anti-rabbit IgG purified antibodies. The preparation method comprises the main steps: coating an immunochromatograohic membrane with the rabbit-anti-LMoV polyclonal antibodies, and preparing the colloidal-gold combined pad by using the immunochromatograohic membrane. The reagent card disclosed by the invention is fast to detect, high in accuracy, strong in specificity, easy to operate and free from need of special equipment and instruments.

Description

technical field [0001] The invention relates to a detection reagent card and a preparation method thereof, in particular to a reagent card for rapidly detecting lily mottle virus (LMoV) by colloidal gold immunochromatography and a preparation method thereof. Background technique [0002] Lily (Lilium spp) is an important economic crop with ornamental, edible and medicinal value. In 2005, the area of ​​the Netherlands, the main producer of ornamental lilies, reached 3800hm 2 , accounting for 72% of the total global area, produces 2.21 billion lily bulbs every year, of which about 100 million are exported to China. The area of ​​edible lilies in Lanzhou City and its surrounding Dingxi, Linxia and other areas in Gansu Province is nearly 10,000hm 2 , more than 90,000 farmers have directly participated in lily planting, and lily has become the main source of income for farmers in production areas. Although the production of lily cut flowers began in my country in the late 1980...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/531
CPCG01N33/558G01N33/56983G01N2333/08
Inventor 张玉宝王亚军郭志鸿谢忠奎王若愚何玉惠
Owner NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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