Fluorescent test paper strip capable of simultaneously testing newcastle disease and bird flu virus and application thereof

A technology for avian influenza virus and Newcastle disease, applied in the field of immunization applications, can solve the problems of difficult recording and preservation of test results, low detection sensitivity, and difficult quantitative detection, and achieve the effects of short detection time, high sensitivity and convenient storage.

Inactive Publication Date: 2010-05-05
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, this method is widely used in the initial screening of samples, but the disadvantage is that the detection sensitivity is low, it is difficult to carry out quantitative detection, and the detection results are not easy to record and save

Method used

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  • Fluorescent test paper strip capable of simultaneously testing newcastle disease and bird flu virus and application thereof
  • Fluorescent test paper strip capable of simultaneously testing newcastle disease and bird flu virus and application thereof
  • Fluorescent test paper strip capable of simultaneously testing newcastle disease and bird flu virus and application thereof

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Embodiment Construction

[0025] In order to further illustrate the present invention, specifically set forth in conjunction with the following examples.

[0026] The method for rapid detection of Newcastle disease virus comprises the steps:

[0027] (1) The present invention utilizes the specificity of the antigen-antibody reaction and the simple and fast principle of fluorescence immunochromatography to establish monoclonal antibodies to Newcastle disease and avian influenza, and uses the reversed-phase microemulsion method to prepare fluorescent nanoparticles with a size of 50 nm as markers.

[0028] (2) Labeling of fluorescent nanoparticles and antibodies: Take 200 μL (0.5 mg / mL) of affinity-purified Newcastle disease or avian influenza monoclonal antibody, and dialyze with 0.025M carbonate buffer solution (pH 9.5) at 4 °C for 6 h; then Add 100 μL of fluorescent nanoparticles (8 mg / mL) suspended in carbonate buffer solution, overnight at 4 °C; add NaBH 3 CN, the final concentration is 5mM, react f...

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Abstract

The invention provides a fluorescent test paper strip capable of simultaneously testing newcastle disease and bird flu virus, belonging to the technical field of animal epidemic disease diagnosis and test methods. The fluorescence labeled test paper strip comprises a sample mat, a combination mat, a nitrocellulose membrane, a absorbent mat and a PVC back lining, wherein the sample mat, the combination mat, the nitrocellulose membrane and the absorbent mat successively adhere to the PVC back lining, a shiplap joint is arranged between the combination mat and the nitrocellulose membrane, a shiplap joint is arranged between the nitrocellulose membrane and the absorbent mat; the combination mat is coated with newcastle disease monoclonal antibody-fluorescent label and bird flu virus monoclonal antibody-fluorescent label, and the nitrocellulose membrane is separately coated with two detection lines composed of newcastle disease monoclonal antibody and bird flu virus monoclonal antibody and a quality control line composed of goat anti-rat IgG. The test paper strip of the invention can be used to simultaneously test two viruses so as to realize the test method capable of testing many viruses in one step, and has the advantages of strong specificity, high sensitivity, short test time (20 minutes), simple operation, etc.

Description

technical field [0001] The invention belongs to the field of immunization application, and relates to a technology for diagnosing or detecting Newcastle disease virus in farms, epidemic prevention and quarantine departments, medical institutions and other units. Specifically, the present invention is a rapid detection method for simultaneously detecting Newcastle disease and avian influenza virus, and a fluorescent test strip for simultaneously detecting Newcastle disease and avian influenza virus. Background technique [0002] Avian influenza (Avian influenza, AI) is a viral infectious disease caused by type A influenza virus that endangers birds, small mammals and humans. It is a disease syndrome ranging from respiratory symptoms to systemic sepsis. Bureau (OIE) as a class A infectious disease. Newcastle disease (Newcastle Disease) is commonly called as " Asian chicken plague ", and Newcastle disease virus (NewcastleDisease Virus, NDV) is the important pathogen that cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
Inventor 邹明强张帆李锦丰
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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