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Method for preparing sheep placenta extract and sheep placenta hydrolyzed collagen concentrated solution

A technology for hydrolyzing collagen and sheep placenta, which is applied in the direction of blood diseases, extracellular fluid diseases, and medical raw materials derived from mammals, etc., can solve the problems of destroying peptide active substances, low biological activity of products, and low utilization rate of raw materials, etc. Achieve the effects of enhancing resistance and immunity, restoring skin beauty, and facilitating popularization and application

Inactive Publication Date: 2012-06-13
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: 1. The waste residue and waste liquid produced in the production process are not used, which is not conducive to environmental protection; The natural peptide actives in sheep placenta, such as hormones, collagen, and immune factors, are separated and then enzymatically hydrolyzed, and the protease also hydrolyzes the above-mentioned actives, so this method destroys the above-mentioned peptide actives, and the produced products have low biological activity; ④ This method only produces one product of sheep placenta, and the remaining substances are not fully utilized, and the utilization rate of raw materials is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing sheep placenta extract and sheep placenta hydrolyzed collagen concentrate by using sheep placenta, the specific steps are as follows.

[0028] (1) Preparation of sheep placenta powder

[0029] Using commercially available freeze-dried sheep placenta as raw material, first put the freeze-dried sheep placenta in a grinder with a cooling jacket, and then pass 4℃ cooling water into the jacket of the grinder at a rotation speed of 1000 rpm. Crush for 5 minutes under the condition of dividing. After the powder is finished, it is sieved with a 40-mesh sample sieve to collect the sieved powder and unsieved particles. The collected sieved powder is used to prepare the crude sheep placenta extract in the next step; the unsieved particles are returned to the pulverizer and then pulverized.

[0030] (2) Preparation of crude sheep placenta extract

[0031] After step (1) is completed, the mass (g) of the powder collected through the 40-mesh sieve collected in step (...

Embodiment 2

[0041] A method for preparing sheep placenta and sheep placenta hydrolyzed collagen concentrate using sheep placenta is the same as that in Example 1, wherein:

[0042] In step (1), the cooling water temperature is 8°C, the crushing time is 10 minutes, and after the powder is crushed, it is passed through a 60-mesh sieve.

[0043] In step (2), the ratio of mass (g) of the sieved powder collected in step (1): volume (mL) of 0.2% NaCl solution is 1:60, the extraction temperature is 6°C, and the extraction time is 4.5 hours. The centrifuge speed is 8000 rpm.

[0044] In step (3), the molecular weight cut-off of the ultrafiltration membrane is 5000 Da, the ultrafiltration pressure is 0.1 MPa, and the high-pressure homogenization pressure is 40 MPa until the volume of the centrifuged supernatant is reduced to 1 / 5 of the original volume.

[0045] The ratio of the mass (g) of the activated carbon: the volume (mL) of the combined liquid is 1:20, the temperature is increased to 90° C., and the...

Embodiment 3

[0051] A method for preparing sheep placenta extract and sheep placenta peptide from sheep placenta is the same as in Example 1, wherein:

[0052] In step (1), the cooling water temperature is 10°C, and the crushing time is 15 minutes. After the powder is crushed, it is passed through an 80-mesh sieve.

[0053] In step (2), the ratio of the mass (g) of the sieved powder (g): the volume (mL) of 0.5% NaCl solution is 1:80, the extraction temperature is 8°C, the extraction time is 5 hours, and the centrifuge rotation speed is 10,000 rpm.

[0054] In step (3), the molecular weight cut-off of the ultrafiltration membrane is 10000 Da, the ultrafiltration pressure is 0.2 MPa, and the high-pressure homogenization pressure is 60 MPa until the volume of the centrifuged supernatant is reduced to 1 / 6 of the original volume.

[0055] The ratio of the mass (g) of the activated carbon: the volume (mL) of the combined liquid is 1:30, the temperature is raised to 100° C., and the mixture is stirred and...

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Abstract

The invention discloses a method for preparing a sheep placenta extract and a sheep placenta hydrolyzed collagen concentrated solution, which belong to the technical field of utilization of sheep placentas. The method comprises the following steps of: preparing sheep placenta powder from commercially-available freeze-dried sheep placentas serving as a raw material; preparing a crude sheep placenta extract extracting solution; and preparing a sheep placenta extract and a sheep placenta hydrolyzed collagen concentrated solution product. The method has the advantages of easiness, easiness and convenience for operating, simple equipment, mild reaction conditions and saving in energy; by adopting the method, a plurality of products such as the sheep placenta hydrolyzed collagen concentrated solution, a feed additive and the like are prepared simultaneously while the sheep placenta extract is prepared, and comprehensive utilization of the sheep placentas is realized; in a production process, substance resources can be fully utilized, 'three wastes' are not discharged, and the environment is protected; and the production cost is low, and popularization and application are facilitated. The method can be widely used for preparing sheep placenta extract and the sheep placenta hydrolyzed collagen concentrated solution, and a product prepared by using the method can be widely applied in the industries of makeups, health-care products and the like.

Description

1. Technical Field [0001] The invention belongs to the technical field of utilization of sheep placenta, and specifically relates to a preparation method for preparing sheep placenta extract and sheep placenta hydrolyzed collagen concentrate by using sheep placenta. 2. Background technology [0002] Sheep placenta extract is the general term for a variety of active substances extracted from sheep placenta, including a variety of proteins, essential amino acids, nucleic acids, hormones, collagen, immune factors, calcium, iron and zinc and other nutrients. It can nourish qi, nourish blood, and replenish essence. Kidney, regulate the body's immunity, delay aging, whiten and rejuvenate skin, maintain body shape and other health care functions. It is clinically used to prevent liver cirrhosis, improve human immunity, treat immune diseases such as viral hepatitis, leukopenia, myasthenia gravis, and malignant tumors. It is also a raw material for cosmetics and tonics with magical effect...

Claims

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Application Information

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IPC IPC(8): A61K35/50C12P21/06A61P1/16A61P7/00A61P21/04A61P35/00A61P37/04
Inventor 徐焕焕周小华王颖利周兴王丹王永红
Owner CHONGQING UNIV
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