Test strip for detecting colibacillus O157:H7 mouse serum antibody by colloidal gold immunochromatography

A technology of O157 and Escherichia coli, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of food safety and human health threats and shortages

Inactive Publication Date: 2010-03-10
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

Rats are closely related to food production and human life, play an important role in the spread of epidemics, and pose a great threat to food saf

Method used

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  • Test strip for detecting colibacillus O157:H7 mouse serum antibody by colloidal gold immunochromatography
  • Test strip for detecting colibacillus O157:H7 mouse serum antibody by colloidal gold immunochromatography
  • Test strip for detecting colibacillus O157:H7 mouse serum antibody by colloidal gold immunochromatography

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Embodiment Construction

[0034] 1 Experimental materials

[0035] Glass cellulose membrane, nitrocellulose (NC) membrane and 20nm particle colloidal gold solution (absorption value at wavelength 521nm is 1.847, A 521 =1.847) was purchased from Shanghai Jiening Biotechnology Co., Ltd.;

[0036] Purified mouse serum IgG was extracted from mouse serum by ammonium sulfate salting out and DEAE cellulose method (Liu Yubin, editor-in-chief of Gou Shijin. Animal Immunology Experimental Technology. Changchun: Jilin Science and Technology Press, 1989, 8-15);

[0037] Purified goat anti-mouse IgG system was injected with purified mouse serum IgG to immunize goats for 4 times, then the immune serum was prepared, and further purified by ammonium sulfate salting out and DEAE cellulose;

[0038]Conjoint bacterium immune rabbit sera were mixed with Salmonella enterica (Salmonella enterica), Escherichia coli (Escherichia coli) K88, K99, O159, O141, TOP10, B121, BJ5183 and Streptococcus suis (Streptococcus suis) SS-1 ...

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Abstract

The invention relates to a test strip for detecting colibacillus O157:H7 mouse serum antibody by colloidal gold immunochromatography, belonging to the technical field of antibody detection kits and consisting of colloidal gold cushion prepared by utilizing a colloidal gold solution the granule of which is 20nm to mark purified sheep anti-mouse IgG, nitric acid cellulose films which are respectively coated by a colibacillus O157:H7 antigen and purified mouse serum IgG and serve as a detection line and a quality control line, a detection test strip formed by assembling a sample absorbing cushionprepared from glass cellulose films and an absorbing cushion made of water absorbing filter paper, positive serum, negative serum, a serum sample diluent and a test strip operating instruction. The test strip is convenient to use, rapid and sensitive, and a detection result can be obtained within 10 minutes, and therefore, the test strip is suitable for on-site detection when infection status ofmouse O157:H7 is surveyed and traced back in animal farmer areas, food production enterprise areas and the like and use when the food safety environment risk evaluation is carried out.

Description

1. Technical field [0001] The invention relates to an Escherichia coli O157:H7 mouse serum antibody colloidal gold immunochromatographic detection test strip, which belongs to the technical field of antibody detection kits. It is specially used for the rapid detection of E. coli O157:H7 antibody in mouse serum, and is suitable for on-site detection when investigating and tracing the infection status of mouse O157:H7 in areas such as animal farms and food production enterprises, as well as for food safety and environmental risk assessment. 2. Background technology [0002] Escherichia coli (E.coli) O157:H7 is an important zoonotic pathogenic microorganism, human infection with O157:H7 can cause diarrhea, hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS) ) and thrombotic thrombocytopenia (TTP) and other serious complications, severe cases can lead to death (Xia Xingxia, Liu Jie, Wang Yongshan, Zhu Yumei, Ouyang Wei, He Kongwang, Zhang Xuehan. Prevention of zoonotic bac...

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/532G01N33/552
Inventor 王永山刘洁夏兴霞何孔旺张雪寒费荣梅
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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