Lateolabrax japonicus embryo stem cell and its culture method

A technology of embryonic stem cells and cells, applied in the direction of embryonic cells, germ cells, tissue culture, etc., to achieve the effect of convenient cultivation, simple operation, and broadening the scope of research

Inactive Publication Date: 2006-02-22
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the establishment of perch embryonic stem cell lines at home and abroad

Method used

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Embodiment Construction

[0020] Describe content of the present invention in detail below by embodiment:

[0021] The embryonic stem cell culture of perch adopts a relatively simple trophoblast-free culture method. More importantly, perch is an important marine cultured species in my country with high economic value. Perch embryonic stem cells can not only be used as model cells of seawater fish to lay a cytological foundation for gene targeting research, but also play an important role in fish functional gene research and site-directed mutation transgenic research. The establishment of a simple and effective method for culturing embryonic stem cells of sea bass is of great significance in the research and practical application of seawater fish genetic engineering breeding.

[0022] Perch embryonic stem cells: cells isolated from the blastocyst stage embryos of perch, characterized by small, round, polygonal or polygonal cells, cell diameters of 15-25um, and large nuclei and less cytoplasm, with high...

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Abstract

The invention provides Lateolabrax japonicus stem cells and the culturing method, which comprises the steps of collecting Lateolabrax japonicus blastula stage cells, embryo stem cells primary culture and subculture, influencing growth of Lateolabrax japonicus embryo stem cells by temperature, culturing embryo stem cells in stationary phase, and identifying the Lateolabrax japonicus embryo stem cell features. The embryo stem cells are cultured with complete medium.

Description

Technical field: [0001] The present invention belongs to cell culture technology in cell biotechnology, and is a technical method for obtaining cells with developmental pluripotency and unlimited proliferation ability--embryonic stem cells under the condition of artificial culture in vitro, using cells at the blastocyst stage of sea bass as materials . Background technique: [0002] Fish embryonic stem cells are undifferentiated cell lines with developmental pluripotency isolated from fish early blastocysts. Before the present invention was made, the international research on the cultivation of fish embryonic stem cells mainly focused on the small model fish zebrafish and medaka: in 1992, Collodi in the United States tried to cultivate zebrafish embryonic stem cells, but failed; in 1995 Sun et al. in the United States obtained the zebrafish embryonic stem cell line by referring to the culture method of mouse embryonic stem cells. The culture method is as follows: use the ze...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12Q1/02C12N5/0735
Inventor 陈松林沙珍霞叶寒青
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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