Lateolabrax japonicus embryo stem cell and its culture method

A technology of embryonic stem cells and cells, applied in the direction of embryonic cells, germ cells, tissue culture, etc., to achieve the effect of convenient cultivation, simple operation, and broadening the scope of research
CN1737130AInactive Publication Date: 2006-02-22YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Publication Date
2006-02-22
Estimated Expiration
Not applicable ยท inactive patent
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Abstract

The invention provides Lateolabrax japonicus stem cells and the culturing method, which comprises the steps of collecting Lateolabrax japonicus blastula stage cells, embryo stem cells primary culture and subculture, influencing growth of Lateolabrax japonicus embryo stem cells by temperature, culturing embryo stem cells in stationary phase, and identifying the Lateolabrax japonicus embryo stem cell features. The embryo stem cells are cultured with complete medium.
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Description

Technical field:

[0001] The present invention belongs to cell culture technology in cell biotechnology, and is a technical method for obtaining cells with developmental pluripotency and unlimited proliferation ability--embryonic stem cells under the condition of artificial culture in vitro, using cells at the blastocyst stage of sea bass as materials . Background technique:

[0002] Fish embryonic stem cells are undifferentiated cell lines with developmental pluripotency isolated from fish early blastocysts. Before the present invention was made, the international research on the cultivation of fish embryonic stem cells mainly focused on the small model fish zebrafish and medaka: in 1992, Collodi in the United States tried to cultivate zebrafish embryonic stem cells, but failed; in 1995 Sun et al. in the United States obtained the zebrafish embryonic stem cell line by referring to the culture method of mouse embryonic stem cells. The culture method is as follows: use the ze...

Claims

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