8results about How to "Improve developmental potential" patented technology

The invention relates to the technical field of embryo culture platforms, in particular to a microfluidic-AI monitoring-metabolism regulation embryo culture platform and a preparation method thereof.The microfluidic-AI monitoring-metabolism regulation embryo culture platform comprises a microfluidic culture system used for achieving embryo culture, metabolite removal and culture medium replacement; the artificial intelligence monitoring system is used for remotely monitoring the embryonic development state in real time and predicting the embryonic development condition; the metabolism regulation and control system is used for feeding back and optimizing culture conditions online according to an artificial intelligence monitoring result and a metabolite analysis result; the intelligent control module is electrically connected with the micro-fluidic culture system, the artificial intelligence monitoring system and the metabolism regulation and control system, and the micro-fluidic culture system can accurately control the culture environment on the micrometer scale, including temperature, pH value and gas composition parameters. The dynamic fluid environment is closer to the in-vivo physiological environment of the embryo. The system can simulate periodic nutrient supply and metabolic waste removal in the uterus, and provides optimal growth conditions for embryos.

A method for cryopreservation and recovery of larch olgensis embryogenic callus relates to the biotechnology field of forest breeding, and comprises the following steps: selecting an embryogenic cell line induced by immature seeds of larch olgensis, and subculturing the embryogenic cell line for 3-5 days to obtain embryogenic callus as a standby material; carrying out gradient pre-culture at the molar concentrations of 0.2 mol / L and 0.4 mol / L in sequence, wherein the culture is carried out at each concentration for 20-48 hours; after pre-culture, putting the embryogenic callus into a freezing tube, and adding a sterile freezing protection solution; and putting the freezing pipe into a programmed cooling box for programmed cooling, and immediately putting the freezing pipe into a liquid nitrogen biological container for storage. The method disclosed by the invention is simple and convenient in operation steps and obvious in effect, the subculture age limit of the larix olgensis embryonic callus is slowed down, and relatively good cell viability and development potential in the initial stage of induction are maintained, so that the occurrence of variation is effectively reduced, manpower and material resources consumed by long-term subculture materials can be saved, and the production cost is reduced. The method is of great significance to theoretical and practical application research of larix olgensis.

The invention relates to the technical field of biology, and provides a composition, a culture solution, a product and a method for promoting embryo ectogenesis. The composition is prepared from 8-Br-cGMP and glycyrrhizic coumarin. The composition can reduce the lipid peroxidation level of embryos and reduce the toxic effect of lipid peroxidation on the embryos. Meanwhile, fat hydrolysis and oxidation energy supply can be promoted, and sufficient energy guarantee is provided for in-vitro development of early embryos. In addition, the composition can effectively reduce the active oxygen level in the embryo and reduce the damage of oxidative stress to the embryo. By adopting the composition disclosed by the invention, the embryo ectogenesis can be promoted, and the in-vitro blastocyst formation rate can be increased.

The invention relates to the technical field of bovine embryo culture media, in particular to a polypeptide-mitochondrial targeting-antioxidant synergistic bovine embryo culture medium and a preparation method thereof.The polypeptide-mitochondrial targeting-antioxidant synergistic bovine embryo culture medium comprises the following components: 200-300 [mu] g / mL of SS-31, 0.1-0.3 mg / mL of superoxide dismutase, 0.5-1.0 mg / mL of heat shock protein mimetic peptide, 20-50 mg / mL of amino acid complex and a basic culture medium, and an efficient mitochondrial protection system is established. The targeting effect of SS-31 and the cooperation of an antioxidant network enable the mitochondrial membrane potential to be maintained at the optimal level, and the ATP yield is increased by nearly 100% and is far superior to that in the prior art. And multi-level defense of oxidative stress is realized. Experimental data show that the ROS level in the culture system is reduced by 45% compared with that of a control group, and lipid peroxidation and protein oxidative damage are remarkably relieved. The protection effect derives from the synergistic effect of multiple antioxidant components.

The invention discloses an application of cordycepin (Cordycepin, Cor) in the aspect of improving the development potential of an in-vitro early embryo. The inventor finds that Cor with a proper concentration is added into a culture medium in the in-vitro maturation process of the porcine oocytes, the Cor can promote in-vitro maturation of the porcine oocytes and improve the quality of the oocytes, and the Cor has a promoting effect on the development potential of early embryos after parthenogenetic activation. Therefore, the inventor constructs a porcine oocyte and early embryo in-vitro culture system, optimizes an in-vitro maturation culture solution formula and a Cor concentration, and further establishes a method for improving the development potential of the in-vitro early embryo, that is, the porcine oocyte is subjected to in-vitro maturation culture, parthenogenetic activation and early embryo in-vitro culture; in-vitro maturation culture liquid containing cordycepin is adopted in the in-vitro maturation culture of the porcine oocytes. In a word, the invention provides a research basis for developing a safe and effective natural small molecule medicament capable of being used for improving the reproductive performance of the sow, provides a reference for in-vitro mass production of high-quality livestock embryos, and has important practical significance in the fields of IVM and IVC.

The present invention discloses a composition, method and application for increasing the number of cells and the proportion of cells in the inner cell mass of in vitro embryos. The present invention aims at the problems of low blastocyst rate, small number of cells in the inner cell mass at the blastocyst stage, low proportion of cells in the inner cell mass, low potential for subsequent embryonic development, etc. in the early development process of in vitro fertilized embryos. By adding one or two regulators of folic acid and sedum B to the culture medium of early embryo in vitro culture, the blastocyst rate, the number of cells in the inner cell mass of the blastocyst and the proportion of the number of cells in the inner cell mass, as well as the survival rate and blastocyst diameter of the delayed culture of the blastocyst are significantly improved, and the potential for in vitro embryonic development is effectively improved. In addition, the present invention also effectively improves the ratio of embryos that can be subsequently transplanted by in vitro fertilized embryos, is low in cost, safe and efficient, and provides a new strategy for the development of in vitro embryo production and assisted reproductive technology for livestock, with broad application prospects.

The application discloses a tissue culture method using maple zygotic embryos as explants and relates to the technical field of plant tissue culture.The method comprises the following steps: S1, cutting the zygotic embryos of immature maple fruits and inoculating the zygotic embryos onto a callus induction culture medium to induce callus; S2, transferring the callus induced in S1 to a bud induction culture medium to induce adventitious buds; and S3, inoculating the adventitious buds formed in step S2 into a strong seedling rooting culture medium to induce rooting, directly forming regenerated seedlings and carrying out transplanting culture.Compared with the culture methods using other maple tissues as explants, the method is more convenient, has higher genotype universality and is more efficient in propagation.

The application belongs to the technical field of genetic engineering, and discloses a method for editing bovine embryos based on freeze-dried sperm and application thereof. The method comprises the following steps: providing bovine sperm; mixing freeze-drying protectants with the bovine sperm, and performing freeze-drying treatment to obtain freeze-dried bovine sperm; designing a TALEN target sequence according to a target gene, constructing a TALEN expression vector, and obtaining TALEN protein targeting the target gene; co-incubating the freeze-dried bovine sperm after rehydration with the TALEN protein to obtain gene-edited bovine sperm; and injecting the gene-edited bovine sperm into bovine oocytes to obtain bovine gene-edited embryos. The bovine gene-edited embryos prepared by the method not only realize effective editing of the target gene, but also exhibit good early embryonic development capacity, thereby providing an efficient, low-cost and popularized technical method for bovine directional genetic breeding.