32results about How to "Improve developmental potential" patented technology

The invention discloses a human ovocyte cryoprotectant. The human ovocyte cryoprotectant contains an ovum refrigerating fluid and an ovum thawing fluid, wherein the ovum refrigerating fluid contains a balance fluid and a refrigerating fluid; the ovum thawing fluid contains a resuscitation fluid, a diluent-1, a diluent-2 and a cleaning solution. The human ovocyte cryoprotectant is developed by combined application of the propanediol and glycol permeable cryoprotectants, and taking the trehalose (trehalose, alpha-D-glucopyranosyl alpha-D-glucopyranoside, C12H22O11) as a non-permeating agent. The non-permeating cryoprotectant suitable for freezing the human ovocyte is developed so that the formation of the ice crystal in the cell can be inhibited, the function of the frozen ovum can be protected and the developmental potentiality of the frozen ovocyte can be greatly improved. The clinical result proves that the fertility rate, the cleavage rate and the high quality blastocyst rate of the frozen ovocyte can be greatly improved.

The invention relates to the technical field of medicines, and provides applications of uterine cavity fluid-derived exosome in preparation of drugs and adjuvant therapeutic agents for treating infertility related diseases. Through the verification of experiments, exosome realizes the treatment or relieving of endometrial growth disorders by promoting the increasing of the thickness of endometrium, enhancing the activity of endometrial cells, reducing the damages to intimal cells, promoting VEGF expression, maintaining the dryness of endometrial stromal cells and stimulating proliferation; andthrough the realizing of the treating or relieving of maternal-fetal immune tolerance disorder-related diseases by achieving immune tolerance effects, treating spontaneous abortion and enhancing T helper lymphocyte levels, a novel theoretical basis can be provided for infertility treatment. In addition, for both fertilized eggs being fertilized in vivo or fertilized in vitro, the exosome can effectively enhance developmental rates in multiples; and implantation rates and farrowing rates of in vitro fertilization transplantation can be increased as well, so that positive auxiliary effects on infertility treatment can be achieved.

The invention discloses a method for improving the parthenogenetic development potential of vitrified mature oocytes, wherein oocytes are frozen in a melatonin-containing freezing liquid, and then arethawed with a melatonin-containing sucrose solution, the thawed oocytes are cultured, the cultured oocytes are subjected to parthenogenetic activation, and finally the parthenogenetically activated embryo is cultured in vitro. According to the present invention, the method has advantages of simple process and short time consumption, and can effectively improve the parthenogenetic activation development potential of vitrified mature oocytes.

The invention relates to a computer assisted embryo selection method. Before ICSI is conducted and when short time insemination oocyte dismantling observation is conducted, Nikon NIS-Elements software is used for collecting oocyte images one by one, the thickness of a transparent tape, the diameter of oocytes and the oocyte gap of a polar body position are measured, and the oocytes with the thickness of the transparent tape being 16.9-20.7 microns, the diameter of the oocytes being 114.8-119.9 microns and the oocyte gap of the polar body position being 8.5-12.3 microns are selected for being subjected to insemination; then embryos with the high developmental potentiality are selected. According to the method, the Nikon NIS-Elements software is used for collecting the oocyte images one by one, the thickness of the transparent tape, the diameter of the oocytes and the oocyte gap of the polar body position are measured, the appropriate oocytes are selected for being subjected to insemination, and then the embryos with the high developmental potentiality are selected. According to the method, a layer of screening is added compared with the prior art, and the embryos with the high developmental potentiality can be better obtained. The data obtained by using the Nikon NIS-Elements software for measuring and analyzing are more accurate.

The invention relates to a method for preparing an in-vitro oocyte maturation culture solution. The preparation method is characterized by comprising the following steps: 1) preparing a basal IVM (InVitro Maturation) culture medium; and 2) adding lipoprotein into the basal IVM culture medium, thereby obtaining the in-vitro oocyte maturation culture solution. According to the method disclosed by the invention, the problems that the developmental potential of oocyte is poor after IVM and developmental potential of embryos is relatively low after parthenogenetic activation can be solved, the embryo can continuously develop to blastula after the lipoprotein is added, the developmental potential of the embryos is improved by the IVM culture solution added with the lipoprotein, and the embryo damage problem is effectively improved.

The invention discloses a method for parthenogenetic activation of buffalo oocytes after vitrification. According to the method, an optimized activation processing manner of combining ionomycin and 6-DMAP is adopted, and the conventional method for parthenogenetic activation of buffalo oocytes after vitrification is improved and optimized. Experiments prove that the parthenogenetic activation condition has a certain influence on the developmental potential of frozen-thawed buffalo oocytes. By applying the method for parthenogenetic activation of frozen-thawed buffalo oocytes, the activation rate can be improved so as to improve the survival rate and developmental potential of the frozen-thawed oocytes.

The invention relates to an oocyte activating physical method and a fertilization potential evaluation index thereof. An electronic pulse sequence is used for activating the oocyte; the calcium oscillation feature formed by calcium ion concentration change in the endochylema detected in a confocal microscopy is used as an oocyte fertilization potential evaluation index.

The invention discloses a polypeptide FFDP and application thereof in oocyte in-vitro culture. The amino acid sequence of the polypeptide FFDP is as shown in SEQ ID NO. 1. Experiments find that the polypeptide FFDP can be applied to PCOS disease affected oocyte development, and especially applied to the aspect of PCOS oocyte in-vitro maturation culture, or applied to the aspect of improving the meiosis defect during oocyte in-vitro maturation culture.

The invention discloses a secreting type endogenous polypeptide PDFF-CO3 and an application thereof. The secreting type endogenous polypeptide PDFF-CO3 is characterized in that an amino acid sequenceof the secreting type endogenous polypeptide PDFF-CO3 is shown as SEQ ID NO. 1. The invention also discloses the application of the secreting type endogenous polypeptide PDFF-CO3 in in-vitro maturation culture of oocytes. The PDFF-CO3 is a CO3 source peptide and is composed of 25 amino acids, and related functions of PDF-CO3 are not reported at present. The PDFF-CO3 is added into a mouse GV-stageegg cell culture solution, so that the biological function of promoting egg cell maturation is achieved; therefore, the PDFF-CO3 can be applied to preparation of medicines for promoting egg cell maturation and improving egg cell development potential and preparation of a culture solution.

The invention discloses a method for improving the success rate of assisted reproduction and a verification method and system.The method for improving the success rate of assisted reproduction comprises the steps that a plurality of culture solutions obtained after blastocyst culture are obtained, and free DNA of embryos on the sixth day to the seventh day is extracted from the culture solutions; the free DNA is used for carrying out embryo uploidy analysis, uploidy scores of all embryos are compared, priority ranking is carried out according to the uploidy scores of the embryos, and the embryos with high priorities have high development potential; according to the method, endometrial receptivity analysis is conducted through an endometrial sample, the receptivity of the endometrial is judged, a corresponding transplantation strategy is made according to a result, the receptivity comprises a receptivity period and a non-receptivity period, and the corresponding transplantation strategy is that the endometrial in the receptivity period state is in a transplantable state, and the endometrial in the non-receptivity period state is in a non-transplantable state; the transplantation time of the endometrium in the non-receptive state is adjusted to reach a transplantable state. The technical scheme can effectively improve the success rate of assisted reproduction.

The invention discloses a method for unfreezing a gamete or an embryo on a frozen carrier.The method sequentially includes the following steps that a vessel special for vitrification unfreezing is prepared, a vessel disc of the vessel special for vitrification unfreezing is provided with an unfreezing liquid hole with the upper portion being open, the left side wall of the unfreezing liquid hole is an inclined plane, and the bottom of the unfreezing liquid hole is a flat bottom; unfreezing liquid is added into the unfreezing liquid hole; a frozen carrying pole is adopted to take the gamete or the embryo out of liquid nitrogen, the gamete or the embryo is located on a blade of the frozen carrying pole, and the blade is made to downwards move in an inclined mode along the left side wall of the unfreezing liquid hole till the blade is soaked in the unfreezing liquid; a frozen carrying pole is utilized to observe that the gamete or the embryo falls.The method has the advantages that the gamete or the embryo on the frozen carrier can be unfrozen rapidly and accurately, so that developmental potentiality and pregnancy outcome of the gamete or the embryo are improved.The invention further discloses the vessel special for vitrification unfreezing.

The invention provides a method for culturing embryonic cells in vitro, and according to the embodiment of the invention, the method comprises the following steps: (i) in vitro culturing fertilized egg cells or derivatives thereof in the presence of an NAD (Nicotinamide Adenine Dinucleotide) signaling pathway agonist. In the culture process of fertilized egg cells or derivatives thereof, the NAD signal channel agonist is added, so that the quality, such as development potential, of embryos cultured in vitro can be effectively improved.

Provided is a fuel oil composition comprising a liquid fuel and carbon black with a particle diameter less than 1 micron. The content of the carbon black is from 0.001 wt % to 4 wt % based on the total weight of the liquid fuel. By way of adding carbon black with the particle diameter less than 1 micron, combustion efficiency of the fuel oil composition used in the internal combustion engine is improved, reducing the emissions of the hydrocarbon and carbon monoxide to reduce greenhouse gas production.