2results about How to "Improve developmental potential" patented technology

The application discloses a tissue culture method using maple zygotic embryos as explants and relates to the technical field of plant tissue culture.The method comprises the following steps: S1, cutting the zygotic embryos of immature maple fruits and inoculating the zygotic embryos onto a callus induction culture medium to induce callus; S2, transferring the callus induced in S1 to a bud induction culture medium to induce adventitious buds; and S3, inoculating the adventitious buds formed in step S2 into a strong seedling rooting culture medium to induce rooting, directly forming regenerated seedlings and carrying out transplanting culture.Compared with the culture methods using other maple tissues as explants, the method is more convenient, has higher genotype universality and is more efficient in propagation.

The application belongs to the technical field of genetic engineering, and discloses a method for editing bovine embryos based on freeze-dried sperm and application thereof. The method comprises the following steps: providing bovine sperm; mixing freeze-drying protectants with the bovine sperm, and performing freeze-drying treatment to obtain freeze-dried bovine sperm; designing a TALEN target sequence according to a target gene, constructing a TALEN expression vector, and obtaining TALEN protein targeting the target gene; co-incubating the freeze-dried bovine sperm after rehydration with the TALEN protein to obtain gene-edited bovine sperm; and injecting the gene-edited bovine sperm into bovine oocytes to obtain bovine gene-edited embryos. The bovine gene-edited embryos prepared by the method not only realize effective editing of the target gene, but also exhibit good early embryonic development capacity, thereby providing an efficient, low-cost and popularized technical method for bovine directional genetic breeding.