Method for improving quality of external fertilization embryo of oocyte of sheep

A technology of oocytes and in vitro fertilization, applied in the direction of artificial cell constructs, germ cells, animal cells, etc., can solve the problems of few, improve the quality of sheep oocytes in vitro fertilized embryos, and the developmental ability of embryos has not been significantly improved.

Inactive Publication Date: 2013-11-27
新疆维吾尔自治区畜牧科学院中国-澳大利亚绵羊育种研究中心
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

③Zygote, 1 (2011), 1-5, author: C.S.Oliveira et al. published "The Effect of Histone Deacetylase Inhibitor TSA in Bovine Embryo Culture in Vitro", and concluded that TSA has a significant effect on the development of bovine 8-cell embryos. No significant improvement in ability
The above-mentioned existing methods are used in somatic cell nuclear transfer and in vitro fertilized embryo culture in cattle, pigs, and mice, but there are few reports on improving the quality of sheep oocytes in vitro fertilized embryos

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving quality of external fertilization embryo of oocyte of sheep

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0025] 1) Remove sheep ovaries that were slaughtered within 30 minutes, put them in normal saline containing 1000IU / ml penicillin and 1000IU / ml streptomycin at a temperature of 32°C, wash them with normal saline three times within 3 hours; A 10ml syringe with a No. 9 needle is used to suck follicles 2-8mm in size from the surface of the ovary, and the follicle fluid recovered from the syringe is placed in a 45mm dish, and the oocytes are picked out under a microscope, and washed with the egg extraction solution for 3 times, then washed 3 times with mature solution, placed in CO 2 Cultivate in the box for 24h, CO 2 The condition of the box is 5% CO 2 , 95% air, temperature 38.6°C; at the end of the cultivation, wash with TSA-containing in vitro fertilization solution for 3 times, and add 30 pieces / drop into 70 μl of TSA-containing in vitro fertilization droplets that were balanced 2 hours ago;

[0026] 2) Thaw the semen in a water bath, transfer it into a test tube of in vitr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for improving quality of an external fertilization embryo of an oocyte of a sheep. The method relates to TrichostatinA (TSA) which is used for treating the oocyte of the sheep and can greatly improve the quality of the external fertilization embryo of the oocyte of the sheep, by adopting the method improving the quality of the external fertilization embryo of the oocyte of the sheep through TSA, after 168 hours, the blastocyst rate and the number of the obtained blastomeres are remarkably higher than those of the matched group, therefore, the method has better scientificity and practicability.

Description

technical field [0001] The invention relates to a method for treating sheep oocytes with Trichostatin A (TSA), which can significantly improve the quality of sheep oocytes in vitro fertilized embryos. Background technique [0002] Trichostatin A (TSA) is a histone deacetylase (Histone deacetylase, HDACs) inhibitor. DNA and histones are the main components of chromatin. Chromatin structure is closely related to gene activity, and chromatin structure is modified through histone acetylation and deacetylation, which plays an important role in DNA replication, gene transcription, and cell cycle regulation. At present, TSA is mainly used in the research of improving the developmental ability of cloned embryos. Its application in mammalian embryonic development in vitro has only been reported in cattle and mice, and there is no report on its application in sheep oocytes. At the same time, the number of blastocyst cells also reflects the quality of embryos to a certain extent. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/075C12N5/071
Inventor 黄俊成汪立芹杨楠林嘉鹏吴阳升唐森赵云程
Owner 新疆维吾尔自治区畜牧科学院中国-澳大利亚绵羊育种研究中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap