Nucleic acid, method and kit for multi-liquid phase gene chip for simultaneously detecting and identifying components of three major categories of poultry, fish and ruminants

Abstract

The invention provides a nucleic acid, method and kit for a multi-liquid phase gene chip for simultaneously detecting and identifying components of three major categories of poultry, fish and ruminants. The nucleic acid comprises universal upstream primers, universal downstream primers, and universal probes for each of the above three categories of species. The method comprises the following steps: 1) extracting an animal genomic nucleic acid from a sample; 2) performing triple PCR amplification on the nucleic acid to obtain an amplification product; 3) performing xMAP triple microsphere suspension hybridization on the amplification product, and detecting a hybridization reaction signal; and 4) according the detection signal, determining a detection result of the sample to be tested. The method of the invention realizes simultaneous detection and identification of components of three major species of poultry, fish and ruminants, avoids separate detection and identification of single species one by one for the same purpose, and significantly reduces the detection cost and the detection time; and the method can efficiently prevent missed inspection and provide technical support for preventing false fraud in food and feed doping, and has important practical value.

Description

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AI Technical Summary

Benefits of technology

This patented method allows us to quickly identify different animal groups by measuring their size or coloration on microscopically small particles suspended within liquid media called droplets that contain DNA fragments from these animals. By comparing this measurement results against known standards, we may determine if there were any more than one type of bird being tested - either wild birds (piglets) or domesticated ones – without having them themselves undergo testing at all. Overall, our inventors aimed towards creating an efficient way to measure large numbers of target molecules accurately while reducing labor requirements and expenses associated with current analysis techniques.

Problems solved by technology

This patents describes two technical aspects involved in identifying animals' sources - wildlife and seafood, while minimizing costs associated with these activities by developing an effective and reliable testing system without requiring expensive laboratory facilities.

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