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a technology of transgenic animals and markers, applied in the field of markers for identifying transgenic animals, can solve problems such as the difficulty of obtaining marker genes that allow easy and reliable identification of transgenic animals
Inactive Publication Date: 2003-08-14
WIMMER ERNST A +2
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However, a major obstacle in the use of these transposons has been the difficulty to obtain marker genes that allow easy and reliable identification of transgenic animals.
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[0009] The following descriptions of particular embodiments and examples are offered by way of illustration and not by way of limitation. Unless contraindicated or noted otherwise, in these descriptions and throughout this specification, the terms "a" and "an" mean one or more, the term "or" means and / or and polynucleotide sequences are understood to encompass opposite strands as well as alternative backbones. The subject methods and applications are applicable to a wide variety of photoreceptor cell or organ bearing animals. By photoreceptive cells or organs is meant any light sensing cell or organ of an animal and include cells such as simple pigmented light sensitive cells or retinular cells and structures such as ocelli also called simple eyes or eye spots like the direct or inverted pigment cups of many worms, structures such as compound eyes found in many arthropods, structures such as complex eyes or camera eyes of cephalopod molluscs and vertebrates. Unless otherwise noted, ...
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Abstract
The invention relates to methods, cells and nucleic acids for making transgenic animals. The methods generally comprise introducing into a genome of an animal a genetic construct comprising a transcriptional regulatory element operably linked to a heterologous marker gene encoding a marker, wherein the element drives expression of the marker across genera transgenic in the construct sufficient to visually detect the marker in photoreceptive cells or organs, and selecting for transgenesis by visually detecting the marker in a photoreceptive cell or organ of the animal.
Description
[0001] This application claims priority under 35USC120 to U.S. Ser. No. 09 / 373,129, having the same title and inventors, filed on Aug. 12, 1999, now U.S. Pat. No. 6,518,481, which is incorporated herein by reference.[0002] 1. Field of the Invention[0003] The field of the invention is markers for identifying transgenic animals.[0004] 2. Background of the Invention[0005] Genetic manipulation of insects and other arthropods is a highly desirable goal for the development of better control strategies to fight agricultural pests and disease vector species. Transposon-based transformation techniques had been available for Drosophila, but only recently did the discovery of new transposons enable this approach in other insects (O'Brochta & Atkinson, 1996, Insect Biochem. Molec. Biol. 26, 739-53), i.e medflies (Loukeris et al., 1995, Science 270, 2002-5; Handler et al., 1998, PNAS 95, 7520-5) and mosquitoes (Coates et al., 1998, PNAS 95, 3748-51; Jasinskiene et al., 1998, PNAS 95, 3743-7). Ho...
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Patent Type & Authority Applications(United States)
IPC IPC(8): C07K14/435
CPCC07K14/43595A01K2217/05
Inventor WIMMER, ERNST A.BERGHAMMER, ANDREAS J.KLINGLER, MARTIN
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