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Halomonas strain H6, composition and application thereof in salt tolerance and growth promoting

A technology of Halomonas and a composition, applied in the field of microorganisms, can solve the problems of poor adaptability and poor effect of related microorganisms, and achieve the effects of promoting crop growth, simple production, obvious root length and fresh weight

Active Publication Date: 2019-06-25
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the arid environment and diverse salinity and alkalinity in Xinjiang, there are problems of poor adaptability and poor effect of related microorganisms

Method used

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  • Halomonas strain H6, composition and application thereof in salt tolerance and growth promoting
  • Halomonas strain H6, composition and application thereof in salt tolerance and growth promoting
  • Halomonas strain H6, composition and application thereof in salt tolerance and growth promoting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Isolation, screening and identification of Halomonas sp. H6

[0040] (1) Separation and screening:

[0041] The rhizosphere soil samples of plants in saline-alkaline desert area of ​​Heshuo County, Xinjiang were collected, and microorganisms were isolated and purified by gradient dilution method. Weigh 10g of the soil sample, put it into 100mL sterile water, add sterilized glass beads, shake at 150rpm, 30°C for 20min, to fully disperse the sample. Aseptically draw 100 μL of the supernatant and add it to 900 μL sterile water, dilute with sterile water to make 10 -2 -10 -5 concentrated diluent. Take 100 μL of the dilutions of each concentration, and use the conventional coating method to spread on the TSA solid medium plate containing 2% NaCl, place it in a constant temperature incubator at 30°C, and cultivate it for 48 hours. After bacteria grow on the plate, pick Take a single colony on the plate for purification and culture until there are no other colon...

Embodiment 2

[0058] Example 2: Detection of growth-promoting ability of Halomonas sp. H6 strain

[0059] (1) Ability to secrete indole acetic acid (IAA)

[0060] Inoculate Halomonas H6 in TSA liquid medium containing 100 mg / L L-tryptophan and 2% NaCl, culture it on a shaker at 180 r / min at 30°C for 1 day, take 50 μL of the bacterial suspension and drop it on a white ceramic plate , while adding an equal volume of Salkowski colorimetric solution, Salkowski colorimetric solution is 50mL 35% HClO 4 and 1mL 0.5mol / L FeCl 3 Mix, and add 50 μL of uninoculated 2% TSA liquid medium and the mixed solution of equal volume colorimetric solution as a control. Place the white ceramic plate at room temperature in the dark for 30 minutes, add 50 μL of Halomonas H6 bacteria suspension, and the color of the white ceramic plate turns red.

[0061] (2) ACC deaminase production test

[0062] Halomonas H6 was inoculated on DF solid medium containing 3mM ACC. After passage for 3 times, Halomonas H6 grew well ...

Embodiment 3

[0064] Example 3: Detection of Salt Tolerance of Halomonas sp. H6 Strain

[0065] Halomonas sp.H6CGMCC No.15758 was inoculated in TSA liquid medium, cultured at 30°C and 180rpm for 24 hours, and the bacterial suspension was respectively inserted into the solution containing 1%, 5%, 10%, 15%, In TSA liquid medium with 20% NaCl concentration, shake culture at 30°C and 180rpm for 24h, measure the OD value at 600nm, and observe the growth of Halomonas sp.H6 to determine its salt tolerance.

[0066] The research results are attached image 3 As shown, in the range of 1-20% NaCl concentration, Halomonas sp.H6 can grow, but with the increase of salt concentration, the growth amount of bacteria shows a significant downward trend,

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Abstract

The invention relates to a novel strain of Halomonas sp. obtained by isolation and screening, which has the ability to secrete indoleacetic acid and produce ACC deaminase. The strain can grow well under low temperature or high temperature and salt and alkali conditions. The composition of the strain and a microbial fertilizer prepared by a liquid fermentation technique alleviate the salt-alkali stress caused by the salt on the crops and promote the growth of the crop under salt stress. The novel strain or the composition provided by the invention is suitable for special geographical and climatic conditions and improves the physical and chemical properties of the soil and the ability to fertilize, effectively utilizes environmental resources and reduces pesticide pollution. The novel strainor the composition has the characteristics of good effect, short cycle and good environmental compatibility, and has important practical value for solving the current soil salinization.

Description

Technical field: [0001] The present invention relates to the field of microorganisms, in particular to a new strain and composition of Halomonas with salt-tolerance and growth-promoting effects, and its application in improving crop salt stress tolerance and promoting crop seedling growth. Background technique: [0002] Soil salinization and secondary soil salinization caused by irrigation is an important ecological environment problem, which usually occurs in areas with arid climate, high soil evaporation intensity, high groundwater table and more soluble salts. Soil salinization has become an important factor that affects the sustainable development of agriculture, animal husbandry and economy in arid and semi-arid areas, and threatens ecological security and stability. [0003] Soil salinization is a major environmental risk caused by natural or human activities. According to the incomplete statistics of UNESCO and FAO, the area of ​​saline-alkali land in the world is 95...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08A01N63/00A01P1/00C12R1/01
Inventor 张志东徐万里顾美英唐琦勇朱静唐光木楚敏宋素琴
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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