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Plant growth-promoting rhizobacteria Gro (rahnella aquatilis) and application thereof

A technology of aquatic Lahnella and rhizosphere growth-promoting bacteria, which is applied in the direction of application, bacteria, and biocides, and can solve the problems of incomplete growth-promoting indicators and low yield of growth-promoting indicators.

Active Publication Date: 2018-09-07
陕西枫丹百丽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The strains disclosed in the above patent documents all have a certain growth-promoting ability, but the growth-promoting indicators are not complete, and the output of prominent growth-promoting indicators is not high.

Method used

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  • Plant growth-promoting rhizobacteria Gro (rahnella aquatilis) and application thereof
  • Plant growth-promoting rhizobacteria Gro (rahnella aquatilis) and application thereof
  • Plant growth-promoting rhizobacteria Gro (rahnella aquatilis) and application thereof

Examples

Experimental program
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Embodiment Construction

[0039] 1. Materials and methods

[0040] 1.1 Experimental materials

[0041] Strain: Gro;

[0042] NA medium: peptone: 10g, beef powder 3g, NaCl 5g, distilled water 1L, pH about 7.2;

[0043] Rhizobium solid medium: yeast extract 1g, K 2 HPO 4 0.5g, MgSO 4 0.2g, NaCl0.1g, mannitol 10g, distilled water 1L, 0.5% Congo red 5ml, agar 20g, pH6.8-7.0;

[0044] Rhizobium liquid medium: yeast extract 1g, K 2 HPO 4 0.5g, MgSO 4 0.2g, NaCl0.1g, mannitol 10g, distilled water 1L, pH6.8-7.0;

[0045] LB liquid medium: yeast powder 5g, tryptone 10g, sodium chloride 10g, agar 20g, pH7;

[0046]Meng Jinna inorganic phosphorus liquid medium: glucose 10g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesium sulfate 0.3g, ferrous sulfate 0.03g, manganese sulfate 0.03g, calcium phosphate 5g, agar 20g, pH7.0-7.2;

[0047] Meng Jinna organic phosphorus liquid medium: glucose 10g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesiu...

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Abstract

The invention relates to plant growth-promoting rhizobacteria Gro (rahnella aquatilis) and application thereof. The plant growth-promoting rhizobacteria Gro is classified and named as rahnella aquatilis, and the preservation number is CGMCC NO.15411. The bacterial strain can produce heteroauxing at high yield; the effect that insoluble potassium-containing silicate is converted into soluble potassium salt is effectively achieved; the organic phosphorus is dissolved; meanwhile, the growth promoting characteristics of fixing nitrogen and generating ACC deaminase, gibberellin, siderophore and thelike are realized; the soil nutrients can be increased; the utilization rate of fertilizers is improved; the absorption of plants on the soil nutrients sis promoted; the bacterial strain can be particularly and successfully subjected to fix planting in cucumber and lettuce rhizosphere soil; the growth of cucumbers and lettuces is promoted; the yield is improved. The bacterial strain Gro has the relatively comprehensive growth promoting function; the IAA producing capability is high; the extreme environment such as acid and alkaline is resisted; high application potential is realized.

Description

technical field [0001] The invention relates to a microbial strain and the application of the strain, in particular to Lahnella aquatica Gro and its application in rhizosphere growth promotion. Background technique [0002] In 1904, Lorenz Hiltner first proposed the concept of rhizosphere, describing the special relationship between bacteria and legume roots. In 1978, plant growth-promotingrhizobacteria (PGPR) was first proposed, mainly referring to a type of bacteria living freely in the soil or attached to plant roots that can promote plant growth and its absorption and utilization of mineral nutrients. Produce metabolic substances to promote plant growth and development, increase crop yield, and inhibit harmful microorganisms to prevent and control diseases (Hussain S. et al., 1999; Kloepper J.W. et al., 1980; Malik K.A. et al., 1997; Schippers B. et al., 1987). PGPR has a variety of growth-promoting mechanisms for plants, mainly including: antagonizing pathogenic bacte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G22/05A01N63/00A01N63/02A01P21/00C12R1/01
CPCA01N63/00A01G22/05A01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 黄英菊周天惠刘辉王灵敏
Owner 陕西枫丹百丽生物科技有限公司
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