Plant growth-promoting rhizobacteria Gro (rahnella aquatilis) and application thereof
A technology of aquatic Lahnella and rhizosphere growth-promoting bacteria, which is applied in the direction of application, bacteria, and biocides, and can solve the problems of incomplete growth-promoting indicators and low yield of growth-promoting indicators.
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[0039] 1. Materials and methods
[0040] 1.1 Experimental materials
[0041] Strain: Gro;
[0042] NA medium: peptone: 10g, beef powder 3g, NaCl 5g, distilled water 1L, pH about 7.2;
[0043] Rhizobium solid medium: yeast extract 1g, K 2 HPO 4 0.5g, MgSO 4 0.2g, NaCl0.1g, mannitol 10g, distilled water 1L, 0.5% Congo red 5ml, agar 20g, pH6.8-7.0;
[0044] Rhizobium liquid medium: yeast extract 1g, K 2 HPO 4 0.5g, MgSO 4 0.2g, NaCl0.1g, mannitol 10g, distilled water 1L, pH6.8-7.0;
[0045] LB liquid medium: yeast powder 5g, tryptone 10g, sodium chloride 10g, agar 20g, pH7;
[0046]Meng Jinna inorganic phosphorus liquid medium: glucose 10g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesium sulfate 0.3g, ferrous sulfate 0.03g, manganese sulfate 0.03g, calcium phosphate 5g, agar 20g, pH7.0-7.2;
[0047] Meng Jinna organic phosphorus liquid medium: glucose 10g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesiu...
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