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A rhizosphere growth-promoting bacterium Lahnella aquatica gro and its application

A technology of aquatic Lahnella and rhizosphere growth-promoting bacteria, which is applied in the direction of application, bacteria, and biocides, and can solve the problems of low yield of growth-promoting indicators and incomplete growth-promoting indicators.

Active Publication Date: 2020-07-24
陕西枫丹百丽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The strains disclosed in the above patent documents all have a certain growth-promoting ability, but the growth-promoting indicators are not complete, and the output of prominent growth-promoting indicators is not high.

Method used

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  • A rhizosphere growth-promoting bacterium Lahnella aquatica gro and its application
  • A rhizosphere growth-promoting bacterium Lahnella aquatica gro and its application
  • A rhizosphere growth-promoting bacterium Lahnella aquatica gro and its application

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Embodiment Construction

[0039] 1. Materials and methods

[0040] 1.1 Experimental materials

[0041] Strain: Gro;

[0042] NA medium: peptone: 10g, beef powder 3g, NaCl 5g, distilled water 1L, pH about 7.2;

[0043] Rhizobium solid medium: yeast extract 1g, K 2 HPO 4 0.5g, MgSO 4 0.2g, NaCl0.1g, mannitol 10g, distilled water 1L, 0.5% Congo red 5ml, agar 20g, pH6.8-7.0;

[0044] Rhizobium liquid medium: yeast extract 1g, K 2 HPO 4 0.5g, MgSO 4 0.2g, NaCl0.1g, mannitol 10g, distilled water 1L, pH6.8-7.0;

[0045] LB liquid medium: yeast powder 5g, tryptone 10g, sodium chloride 10g, agar 20g, pH7;

[0046]Meng Jinna inorganic phosphorus liquid medium: glucose 10g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesium sulfate 0.3g, ferrous sulfate 0.03g, manganese sulfate 0.03g, calcium phosphate 5g, agar 20g, pH7.0-7.2;

[0047] Meng Jinna organic phosphorus liquid medium: glucose 10g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesiu...

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Abstract

The invention relates to a rhizosphere growth-promoting bacterium Gro and its application, which is classified as Rahnella aquatilis and has a preservation number of CGMCC NO.15411. The bacterial strain of the present invention can produce high indole acetic acid, can effectively solve the problem of converting insoluble potassium-containing silicates into soluble potassium salts, decompose organic phosphorus, and simultaneously fix nitrogen, produce ACC deaminase, gibberellin, and siderophiles. It can increase soil nutrients, improve fertilizer utilization rate, and promote plants to absorb soil nutrients. It can especially successfully colonize cucumber and lettuce rhizosphere soil, promote the growth and increase yield of cucumber and lettuce. The bacterial strain Gro of the present invention has a relatively comprehensive growth-promoting function, has a strong ability to produce IAA, is resistant to extreme environments such as acid and alkali, and has great application potential.

Description

technical field [0001] The invention relates to a microbial strain and the application of the strain, in particular to Lahnella aquatica Gro and its application in rhizosphere growth promotion. Background technique [0002] In 1904, Lorenz Hiltner first proposed the concept of rhizosphere, describing the special relationship between bacteria and legume roots. In 1978, plant growth-promotingrhizobacteria (PGPR) was first proposed, mainly referring to a type of bacteria living freely in the soil or attached to plant roots that can promote plant growth and its absorption and utilization of mineral nutrients. Produce metabolic substances to promote plant growth and development, increase crop yield, and inhibit harmful microorganisms to prevent and control diseases (Hussain S. et al., 1999; Kloepper J.W. et al., 1980; Malik K.A. et al., 1997; Schippers B. et al., 1987). PGPR has a variety of growth-promoting mechanisms for plants, mainly including: antagonizing pathogenic bacte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01G22/05A01N63/20A01P21/00C12R1/01
CPCA01N63/00A01G22/05A01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 黄英菊周天惠刘辉王灵敏
Owner 陕西枫丹百丽生物科技有限公司
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