A kind of composite microbial bacterial agent, bio-organic fertilizer and its preparation method and application
A technology of compound microbial bacterial agent and bacillus, which is applied in the field of bio-organic fertilizer and its preparation, and compound microbial bacterial agent, can solve the problems of endangering the root system of crops, incomplete treatment, and death of crops, etc. High tolerance, no mutual phagocytic effects
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[0054] The present invention does not have special limitation to the preparation method of described composite microbial bacterial agent, preferably comprises the following steps:
[0055] (1) respectively activating the described Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium and Aspergillus oryzae to obtain activated strains respectively;
[0056] (2) The activated bacterial classification obtained respectively in the step (1) is mixed with the seed liquid to obtain the first-grade seed liquid respectively;
[0057] (3) mixing the first-level seed liquid obtained in the step (2) with the fermentation medium, respectively carrying out the first fermentation to obtain the first-level fermentation liquid respectively;
[0058] (4) carrying out the second fermentation of the first-stage fermented liquid obtained respectively in the step (3), respectively obtaining the second-stage fermented liquid;
[0059] (5) carrying out the third ...
Embodiment 1
[0089] In terms of mass percentage, the composite microbial bacterial agent is compounded with 20% of Bacillus amyloliquefaciens, 10% of Bacillus licheniformis, 35% of Bacillus subtilis, 20% of Bacillus megaterium and 15% of Aspergillus oryzae to obtain the composite microbial bacterial agent. Among them, the effective number of viable bacteria of Bacillus amyloliquefaciens is 2×10 9 cfu / g; the effective number of viable bacteria of Bacillus licheniformis is 0.5×10 9 cfu / g; the effective number of viable bacteria of Bacillus subtilis is 1×10 9 cfu / g; the effective number of viable bacteria of Bacillus megaterium is 2×10 9 cfu / g; the effective number of spores of Aspergillus oryzae is 10×10 9 cfu / g.
Embodiment 2
[0091]In terms of mass percentage, the composite microbial bacterial agent is compounded with 20% of Bacillus amyloliquefaciens, 10% of Bacillus licheniformis, 35% of Bacillus subtilis, 20% of Bacillus megaterium and 15% of Aspergillus oryzae to obtain the composite microbial bacterial agent. Among them, the effective number of viable bacteria of Bacillus amyloliquefaciens is 5×10 9 cfu / g; the effective number of viable bacteria of Bacillus licheniformis is 2×10 9 cfu / g; the effective number of viable bacteria of Bacillus subtilis is 2×10 9 cfu / g; the effective viable count of Bacillus megaterium is 2.5×10 9 cfu / g; the effective number of spores of Aspergillus oryzae is 15×10 9 cfu / g.
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