Method for separating plant growth-promoting rhinoacteria on copper tailings and evaluating performances of plant growth-promoting rhinoacteria

Abstract

The invention discloses a method for separating 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase growth-promoting bacteria produced by plant on copper tailings, and evaluating the performances of the 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase growth-promoting bacteria. Various strains for producing ACC deaminase are separated from the root system of the phytocoenosium growing on the copper tailings, and the evaluating for the performances and applications comprise the evaluating for the individual performances of the separated strains and the influences of the strains for the growth of native plants and the exotic plants. The separated various strains can secrete ACC deaminase, IAA deaminase and siderophore and have the phosphorus dissolubility, the separated strains can be used for repairing copper tailings areas, and the prepared microbial modifier has the great acceleration effect for the native plants and exotic plants growing on the copper tailings.

Description

[0001] 【Technical field】 [0002] The invention relates to the field of restoration of heavy metal tailings, in particular to a method for the separation and performance evaluation of plant rhizosphere growth-promoting bacteria on copper tailings. [0003] 【technical background】 [0004] The ecological environment and social development are major issues of concern to the international community at present. A series of ecological, social and economic issues caused by the development and utilization of mineral resources are one of the focuses of environment and development. Abandoned mines not only destroy and occupy a large amount of land resources, but also often contain a variety of pollutants in tailings, such as extreme pH, high content of heavy metals, and harmful mineral materials. The area has brought serious ecological environmental pollution. Heavy metal pollution in mine waste is the most serious. Because heavy metals are not easy to decompose, transform or enrich aft...

AI Technical Summary

Problems solved by technology

[0008] The object of the present invention is to isolate and produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase bacterial strain (hereinafter referred to as ACC bacterial strain) from the root system of the plant community that grows on copper tailings, and plant community comprises imperata cylindrica community (imperata cylindrica ), bermudagrass community (cynodon dactylon), evening primrose community (oenothera erythrosepala) and erect astragalus community (astragalus adsurgens) and other common communities, obtaining excellent strains and applying them to the field of tailings restoration can make plants better Planting on the tailings soil to overcome the deficiency that plants cannot grow or grow poorly on the tailings in the prior art

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