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Method for preparing seaweed plant growth regulator by using whole biological enzymolysis method

A technology of plant growth regulators and biological enzymes, applied in the direction of plant growth regulators, plant growth regulators, botany equipment and methods, etc., can solve the problem of product activity reduction, achieve molecular weight reduction, scientific and efficient production process, The effect of high retention

Inactive Publication Date: 2017-10-10
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing enzymatic hydrolysis technology process, the raw material seaweed needs to go through a complicated pretreatment process to obtain fine particles of seaweed to facilitate enzymolysis.
These pretreatments include seaweed soaking, cleaning, impurity removal, shearing, grinding, etc. A large amount of active substances in seaweed such as mannitol, iodine, fucoidan and others are lost in these pretreatment processes, resulting in the activity of the final product. reduce

Method used

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  • Method for preparing seaweed plant growth regulator by using whole biological enzymolysis method

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Effect test

Embodiment 1

[0034] A method for preparing a seaweed plant growth regulator by an overall biological enzymatic hydrolysis method, comprising the following steps:

[0035] 1) Preparation before production

[0036] ① Prepare raw materials: prepare complete dried seaweed, cellulase, fucoidanase, protease, pectinase, amylase;

[0037] ②Preparation of equipment and tooling: preparation of enzymolysis tanks with temperature control devices, colloid mills or homogenizers, ultrasonic oscillation equipment, horizontal screw discharge sedimentation centrifuges;

[0038] ③Preparation of process auxiliary materials: monohydroxyethylamine, 5% potassium hydroxide by mass fraction, monochlorotriazine-β-cyclodextrin;

[0039] 2) Pretreatment of raw materials:

[0040] Soak the dried seaweed as a whole in an enzymatic hydrolysis tank of 10 times the weight of the water, wherein the water contains potassium sulfite of 5% by weight of the dry seaweed and mix evenly, and soften the seaweed for 2.5 hours at ...

Embodiment 2

[0072] A method for preparing a seaweed plant growth regulator by enzymatic hydrolysis as a whole is generally consistent with Example 1, the difference being:

[0073] 2) Pretreatment of raw materials:

[0074] Soak fresh seaweed as a whole in water twice its weight and put it into an enzymolysis tank, wherein the water contains 0.3% of the weight of fresh seaweed inorganic salts and mix evenly, soften the seaweed for 1 hour at a temperature of 40°C and intermittent stirring to obtain a A seaweed slurry with a pH of 6.5;

[0075] 3) Primary enzymatic hydrolysis

[0076] Control the temperature at 35°C, add 4% cellulase and 0.3% fucoidanase to the seaweed slurry, stir intermittently, and enzymatically hydrolyze for 6 hours, and end the primary enzyme when the pH of the hydrolyzed solution is 5 untie;

[0077] 5) Homogeneous enzymolysis solution

[0078] After completing step 3) and step 4), the enzymatic seaweed slurry that has been basically liquefied is further refined t...

Embodiment 3

[0089] A method for preparing a seaweed plant growth regulator by enzymatic hydrolysis as a whole is generally consistent with Example 1, the difference being:

[0090] 2) Pretreatment of raw materials:

[0091] Soak the dried seaweed as a whole in an enzymatic hydrolysis tank of 5 times the weight of the water, wherein the water contains 5% potassium sulfite by weight of the dry seaweed and mix them uniformly, and soften the seaweed for 3 hours at a temperature of 60°C and intermittent stirring to obtain a A seaweed slurry with a pH of about 7.

[0092] 3) Primary enzymatic hydrolysis

[0093] Control the temperature at 50°C, add 1% cellulase and 0.3% fucoidanase based on the dry weight of seaweed to the seaweed slurry, stir intermittently, and the enzymolysis time is 6h. At this time, the pH of the enzymolysis solution is 6.5, and the end primary enzymatic hydrolysis;

[0094] 5) Homogeneous enzymolysis solution

[0095] After completing step 3) and step 4), the enzymati...

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Abstract

The invention relates to a method for preparing a seaweed plant growth regulator by using a whole biological enzymolysis method. The method comprises the nine steps of preparation before production, pre-treatment of material, primary enzymolysis, enzymatic hydrolysate edulcoration, enzymatic hydrolysate homogenization, secondary enzymolysis, post-treatment, separation and purification of somatotropin and final fertilizer configuration. According to the method provided by the invention, commodity complex enzyme is used for performing enzymolysis on the seaweed, complexity of pre-treatment of enzymolysis technology, such as seaweed soaking, cleaning, edulcorating and grinding and the disadvantage that active material is lost due to caused by the pre-treatment are overcome, the fresh seaweed or the dried seaweed are directly, integrally, tenderly and efficiently softened and enzymatically decomposed, active material in the seaweed is furthest reserved, and effect and quality of seaweed fertilizer are ensured.

Description

technical field [0001] The invention relates to the field of agricultural fertilizers, in particular to a method for preparing a seaweed plant growth regulator by an integral biological enzymolysis method. Background technique [0002] As early as the middle of the 12th century AD, seaweed fertilizer had been widely used by people in Western Europe and other places. In the 17th century, the French government strongly recommended the use of seaweed as soil fertilizer in coastal areas. In 1880, some scholars first reported the comparative test results of using seaweed extract, which clearly demonstrated the superiority of seaweed as a fertilizer. A large number of application effects and studies have shown that seaweed extracts have many advantages when applied to crop production: it can increase seed germination rate, promote seedling growth, improve yield and quality, increase crop stress resistance and promote the formation of soil humus after crop harvest. These outstand...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00A01N65/03A01P21/00
Inventor 王海英管菲冷凯良苗钧魁于景晴
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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