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Bacillus megaterium

A technology of Bacillus megaterium and strains, applied to Bacillus megaterium and its application fields, can solve the problems of few pure Bacillus megaterium strains, limit the application of high-quality growth-promoting Bacillus strain resources, etc. Effect

Active Publication Date: 2021-01-15
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] At present, in the black soil region of Northeast my country, there are relatively few pure Bacillus strains with the ability to secrete IAA, phosphorus solubilizing ability and ACC deaminase activity, which limits the availability of high-quality growth-promoting Bacillus strains suitable for the black soil region in Northeast China. application

Method used

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Embodiment Construction

[0029] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0030] The present embodiment proposes a method for isolation and identification of bacillus, comprising the following steps:

[0031] Step 1: Strain isolation and purification

[0032] After sampling, the black soil soil samples were pretreated, specifically: adding the black soil soil samples into a triangular flask filled with sterile water, ultrasonicating for 1 min, then shaking at 200 rpm for 45 min, then standing for 20 min, then absorbing the suspension and adding it ...

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Abstract

The invention discloses bacillus megaterium. A 16S rDNA sequence length of the bacillus megaterium is 1430bp, and a nucleotide sequence of the bacillus megaterium is shown as SEQ ID NO: 1. According to the invention, the separated and purified bacillus megaterium is bacillus with IAA secretion capability, phosphorus dissolving capability and the ACC deaminase activity; and the bacillus megateriumwith the IAA secretion capability, the phosphate dissolving capability and the ACC deaminase activity has important application in the aspects of promoting plant growth and dissolving insoluble phosphorus-containing compounds in soil. Theoretical basis and technical guarantee can be provided for construction of a high-quality bacillus strain resource library and agricultural sustainable development.

Description

technical field [0001] The invention relates to the field of microbial engineering, in particular to a bacillus megaterium and its application. Background technique [0002] Bacillus megaterium is a class of aerobic or facultatively anaerobic Gram-positive bacteria that can produce stress-resistant spores. The "megaterium" in its name implies that the bacteria are large and are considered to be the largest bacteria in the genus Bacillus. Bacillus megaterium has the characteristics of many types, strong genetic stability, high temperature resistance and strong stress resistance. It has the functions of preventing and controlling plant diseases and insect pests, secreting plant hormones and dissolving insoluble phosphorus-containing compounds in soil. Resource microorganisms with growth-promoting function have very broad application prospects in scientific fields such as industry, agriculture and medicine; [0003] At present, in the black soil region of Northeast my country...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P21/00C09K17/14C12R1/11C09K101/00
CPCC12N1/20A01N63/22C09K17/14C09K2101/00C12R2001/11C12N1/205
Inventor 王光华于镇华刘俊杰金剑刘晓冰
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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