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Method for improving drought enduring capacity of plants

A plant, deaminase technology, applied in the directions of botanical equipment and methods, biochemical equipment and methods, plant products, etc., can solve the drought resistance of ACC deaminase There are not many reports and proofs, ACC deaminase homology Genes are not reported, etc.

Pending Publication Date: 2019-06-04
SYNGENTA PARTICIPATIONS AG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are not many reports and proofs for the direct evidence of ACC deaminase in plants in plant drought resistance
On the other hand, no homologous genes of ACC deaminase in plants have been reported.

Method used

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  • Method for improving drought enduring capacity of plants
  • Method for improving drought enduring capacity of plants
  • Method for improving drought enduring capacity of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Example 1. Overexpression of ACC deaminase from Burkholderia caledoniae (BcaLMG19076-ACCD, SEQ ID NO:5-7) in maize

[0096] 1.1. Construction of binary vector 20049

[0097]The ACC deaminase (BcaLMG19076-ACCD, SEQ ID NO:6-7) in the Burkholderia caledonia LMG19076 was called out from the NCBI database; its sequence was carried out to maize codon optimization (SEQ ID NO:5), designed with BsmBI restriction sites at both ends, and synthesized in GenScript. After digestion with BsmBI, it was ligated into the intermediate vector of BamHI / SpeI fragmentation. Spectinomycin-positive clones (Spec+) were verified by PvuI / NotI digestion. The correct clone was further verified by sequencing that the connection was correct. Maize ubiquitin promoter (SEQ ID NO:4), for the expression of BcaLMG19076-ACCD ( figure 1 ).

[0098] 1.2. Agrobacterium transformation of maize

[0099] The maize transformation variety in the experiment was JHAX. The transformation method of Agrobacterium...

Embodiment 2

[0121] Example 2. Overexpression of BcaLMG19076-ACCD improves corn water use efficiency

[0122] Water Use Efficiency (WUE) is the amount of water needed to accumulate dry matter per unit weight; the less water needed reflects the improvement of the ability of plants to avoid drought. In the early stage of drought and / or under mild drought, the stronger the drought avoidance ability, the better the plants can cope with drought stress.

[0123] The T2 generation seeds of 3 transgenic events were selected for water use efficiency analysis (Table 7). 50 transgenic and non-transgenic T2 generation seeds were picked out by the red fluorescent marker (RFP) in the aleurone layer of the seeds. Prepare a 9cm x 9cm square pot and fill it with culture soil of similar weight. The T2 seeds were broadcast live in the middle of the square pot, 1 cm deep. Water and fertilize normally until it grows to V3 stage. Select 30 transgenic plants and 30 non-transgenic plants of similar size; plac...

Embodiment 3

[0127] Example 3. Overexpression of BcaLMG19076-ACCD increases maize drought resistance

[0128] Drought resistance means that plants can maintain a certain growth and yield under drought stress by adjusting their own water potential, reducing the concentration of active oxygen, and reducing the rupture of cell membranes. Severe drought will lead to crop failure, and severe drought is often avoided in actual production. Therefore, under moderate drought stress, it is very important to increase crop yield stability by improving crop drought resistance. Moderate drought is defined on maize as a stress condition that reduces field yield by 40-60%.

[0129] In the greenhouse, paired drought experiments were used to test differences in plant tolerance to drought. Due to the significant correlation between biomass and yield, drought stress treatments with 40-60% biomass reduction in paired drought experiments were defined as moderate drought accordingly. Under this condition, T2 ...

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Abstract

The application of the invention relates to the field of genetic modification and gene editing, in particular to genetic modification and gene editing in plants such as crops. The invention provides amethod for improving the drought resistance of the plants through ACC deaminase in specific expression. Through the adoption of the method disclosed by the invention, the drought enduring capacity ofthe plants, such as the crops especially corn, can be improved.

Description

technical field [0001] The invention provides a method for improving the drought tolerance of plants by specifically expressing ACC deaminase. Background technique [0002] The continuous growth of population has brought two challenges to the sustainable development of agriculture. One is that more people need to consume more crop products, and crop production requires a lot of water resources. Yet another challenge is that a growing population will compete for more water available, further exacerbating the water deficit for agriculture. To ensure the sustainability of agricultural production, it is necessary to increase the water use efficiency of crops. Breeding drought-tolerant crops is an important way to maintain agricultural sustainability. [0003] Plants have evolved three different mechanisms to adapt to drought stress [1]. Drought evasion plants can generally avoid drought stress by regulating the growth period, lagging or advancing the period of more water use...

Claims

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Application Information

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IPC IPC(8): C12N9/78C12N15/55A01H5/00A01H6/46A01H6/20A01H6/54A01H6/82A01H6/02A01H6/14
Inventor 吕建尚永申刘志强孙跃进尼古拉斯·贝特
Owner SYNGENTA PARTICIPATIONS AG
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