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A strain of Bacillus welchii and its application

A technology of Bacillus and Bacillus, applied in Bacillus wilsonii and its application fields, can solve the problems of soil having no treatment value, soil pollution, threatening higher organisms, etc.

Inactive Publication Date: 2020-12-01
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Heavy metals cannot be biodegraded, but they are bioaccumulative and can directly threaten higher organisms including humans. Relevant experts pointed out that heavy metal pollution to soil is irreversible, and the contaminated soil has no treatment value, and can only be avoided by adjusting planting varieties

Method used

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  • A strain of Bacillus welchii and its application
  • A strain of Bacillus welchii and its application
  • A strain of Bacillus welchii and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Screening and identification of a strain of Bacillus wilsonii

[0015] 1. Domestication of water hyacinth

[0016] The water hyacinth lotus used in the experiment was collected from Nanhu Lake, Hongshan District, Wuhan City. After the harvested water hyacinths were rinsed with tap water, they were put into tap water for pre-cultivation for one week. Select water hyacinths that are basically the same in size and growth, and rinse them with deionized water. Then put water hyacinth in Hoagland nutrient solution containing 2.0 mg / L Cd ion and cultivate them for 4 weeks. Obtain a domesticated water hyacinth plant.

[0017] 2. Isolation of a strain of Bacillus wilsonii

[0018] Obtain domesticated plants and select the roots with high enrichment for strain screening: Rinse the roots of water hyacinth hyacinth stored in a -80°C refrigerator three times with deionized water, then carry out surface disinfection, and immerse the intact plant tissues in 75°C. % etha...

Embodiment 2

[0022] Example 2 Bacterial strain LX5 to the maximum tolerance concentration of heavy metals

[0023] Prepare solid medium containing heavy metal ions, the concentration starting from 0.1mM / L and increasing by 0.5mM. Streak inoculation on fresh LB solid medium, incubate at 30°C for 48 hours for activation, pick a single colony and inoculate into LB liquid medium, culture at 30°C, 120r / min shaking for 24h, collect bacteria with a bacterial concentration of OD600 of 1.5 liquid press 100, 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Proportionally dilute, dip the bacterial solution of each concentration and inoculate it on the heavy metal solid medium. Cultivate at 30°C, observe and record the growth of the bacteria every 24 hours, and see Table 1 for the lowest heavy metal concentration that inhibits the obvious growth of the bacteria within 120 hours, that is, the maximum tolerated concentration of the bacteria.

[0024] Table 1-statistical table of bacterial strain tolerance concen...

Embodiment 3

[0026] Example 3 Strain LX5 improves the enrichment ability of water hyacinth to heavy metals

[0027] Water hyacinth was cultured in Hoagland nutrient solution containing 2.0 mg / L Cd ions for 15 days, and strain LX5 was added after adding no exogenous bacteria to focus on the enrichment ability of heavy metals.

[0028] As a result, compared with the control group, the enriched Cd content in the roots of the exogenously added strain LX5 increased by 46.3% from 0.498mg / L to 0.729mg / L; the stem and leaf part increased from 0.090mg / L to 0.149mg / L, an increase of 64.4%.

[0029] Table 2

[0030] Cd (mg / L) the root Stems and leaves test group 0.729 0.149 control group 0.498 0.09

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Abstract

The invention provides a strain of Bacillus wilsonii, and the preservation number of the strain is CCTCC NO.M2019204. The present inventor screened a strain of microorganisms from the roots of domesticated water hyacinth, found that the strain had strong resistance to heavy metals, and was identified as Bacillus wiedmannii strain by colony morphology, biochemistry and 16S rDNA sequencing analysis. ), the strain of Bacillus welchii can improve the ability of water hyacinth to accumulate heavy metals, and the heavy metals include at least one of Cd, Zn, Mn, Ni, Cu, and Pb. At the same time, it can release IAA and ACC deaminase to promote the growth of water hyacinth.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of bacillus wilsonii and its application. Background technique [0002] Heavy metals originally refer to metals with a specific gravity greater than 5 (metals with a density greater than 4.5 grams per cubic centimeter), including gold, silver, copper, iron, lead, etc. Heavy metal pollution is different from pollution from other organic compounds. Many organic compounds can reduce or eliminate their harmfulness through physical, chemical or biological purification of nature itself. Heavy metals are enriched and difficult to degrade in the environment. At present, due to the mining, smelting, and processing of heavy metals in China, many heavy metals such as lead, mercury, cadmium, and cobalt enter the atmosphere, water, and soil, causing serious environmental pollution. For example, heavy metals discharged with wastewater, even if the concentration is small, can accumulat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34A01N63/22A01P21/00C12R1/07C02F101/20
CPCA01N63/00C02F3/34C02F2101/20C12N1/205C12R2001/07
Inventor 万小琼徐腾起李赟泽朱端卫赵建伟周文兵华玉妹刘广龙胡金龙肖乃东
Owner HUAZHONG AGRI UNIV
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