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Preparation method and application of potassium-dissolving microbial agent

The technology of a microbial inoculum and potassium-solubilizing bacteria can be applied in the field of microorganisms, which can solve the problems of unfavorable agricultural sustainable development, easy damage to soil structure, and decrease of organic matter content, and achieves the effects of promoting the growth of ryegrass, low cost and increasing content.

Inactive Publication Date: 2018-10-23
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical potassium fertilizers are often used in agricultural production to provide the potassium elements needed for crop growth, but it is easy to damage the soil structure and lead to a decrease in organic matter content, which is not conducive to the sustainable development of agriculture
[0003] 95% of the potassium in the soil is in the form of mineral potassium, which is commonly found in two major minerals, potassium feldspar and mica, and cannot be directly absorbed and utilized by plants.

Method used

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  • Preparation method and application of potassium-dissolving microbial agent
  • Preparation method and application of potassium-dissolving microbial agent
  • Preparation method and application of potassium-dissolving microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Embodiment 1: the potassium decomposing ability of the potassium decomposing microbial bacterial agent

[0030] Using spot seeding method, Aspergillus aculeatus was inoculated into solid medium containing 1% insoluble potassium (potassium feldspar powder), and cultured upside down at 30°C for 7 days to observe the change of colonies in the medium. The result is as figure 1 As shown, a white potassium-dissolving circle appeared around the colony, indicating that Aspergillus aculeatus fungus has a potassium-dissolving effect on insoluble potassium.

[0031] Put the Aspergillus aculeatus solution (the number of bacteria in the solution is in the range of 1×109~9×109 CFU / mL) into the liquid medium containing potassium feldspar powder at an inoculum of 3%, culture it in a triangular flask, and use flame photometry The concentration of soluble potassium in the fermentation broth was determined by meter method.

Embodiment 2

[0032] Embodiment 2: the ability of organic acid secreted by potassium decomposing microbial bacterial agent

[0033] The principle of dissolving potassium by potassium-dissolving bacteria is to secrete organic acids, lower the pH value of the rhizosphere environment, and thus dissolve insoluble potassium in the soil into soluble potassium for direct absorption and utilization by plants. The types and concentrations of organic acids in the fermentation broth were determined by high performance liquid chromatography using potassium feldspar powder liquid medium.

Embodiment 3

[0034] Embodiment 3: the preparation of potassium decomposing microbial bacterial agent and be used for ryegrass pot experiment

[0035] Put the bacterial liquid of the potassium-solubilizing bacterium Aspergillus aculeatus (the number of bacteria in the bacterial liquid is in the range of 1×109~9×109 CFU / mL) into 100mL Martin’s (Martin) liquid medium at an inoculum of 3%, at 30°C , 170 r / min shaking culture for 48 hours, that is, the liquid potassium decomposing bacteria solution was obtained. Add 20mL of the above liquid potassium-dissolving bacteria solution into a solid medium (v / v=2:1) ​​mixed with 100g of sand and peat, adjust the pH to 6, and incubate at 30°C for 3 days to make a solid potassium-dissolving microbial agent.

[0036] The ryegrass pot experiment set up a treatment group and a control group. The treatment group was treated with 5g / pot of the above-mentioned solid microbial agent (500g of potassium-deficient and insoluble potassium soil in each pot), and the...

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Abstract

The invention discloses a preparation method and application of a potassium-dissolving microbial agent, and belongs to the field of microbial technology. The preparation method of the potassium-dissolving microbial agent comprises: 1, preparation of a liquid potassium-dissolving microbial liquid; and 2, preparation of a solid potassium-dissolving microbial agent. The application of the solid potassium-dissolving microbial agent includes: (1) during planting, the solid potassium-dissolving microbial agent is applied by a broadcast mode, and the application amount is 10-20 kg per acre; (2) during sowing, the solid potassium-dissolving microbial agent and seeds undergo seed dressing, and 5-8 kg of the solid potassium-dissolving microbial agent is mixed in 1 kg of ryegrass seeds for sowing. The potassium-dissolving microorganism, namely Aspergillus aculeatus, has compatibility with ryegrass, has the capability of secreting IAA, siderophore, ACC deaminase and organic acid, and can effectively promote growth of the plant while dissolving potassium, can improve the soil fertility, and can greatly increase the content of rapidly available potassium in the soil. The preparation is convenient, the application method is simple, and the product can be inoculated by means of broadcast or seed dressing. The cost is low and the operation is simple.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation method and application of a potassium-dissolving microbial bacterial agent. Background technique [0002] Potassium is one of the important nutrient elements for crop growth. When it exists in an appropriate amount, the plant's enzymes can fully play its role. As an essential nutrient element for plant growth, potassium can promote the photosynthesis of plants and promote the growth of plants. According to the soil census data, the ratios of potassium deficiency and serious potassium deficiency in my country's cultivated land are about 70% and 45% respectively, and the ratio of nitrogen, phosphorus and potassium in fertilizers is 1:0.4:0.16, which is much smaller than that of developed countries (1:0.5: 0.4, has become an important factor limiting crop yield and quality improvement. Chemical potassium fertilizers are often used in agricultural pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N11/14A01N63/04A01P21/00A01C1/06A01G7/06A01B79/02
CPCA01B79/02A01C1/06A01G7/06A01N63/30C12N1/14C12N11/14
Inventor 傅金民谢燕李晓宁邵安徐筱周秀文赵春程王金辉邢美玲任路杰
Owner LUDONG UNIVERSITY
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