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Preparation method of biological decomposition agent

A decomposing agent and biological technology, applied in the agricultural field, can solve problems such as low decomposing efficiency, and achieve the effects of improving soil structure, increasing soil nutrients and improving efficiency.

Inactive Publication Date: 2018-04-06
常州文诺纺织品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a preparation method of a biological decomposing agent in view of the problem that the current organic material decomposing agent is accompanied by the generation of malodorous gas and the decomposing efficiency is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0034] A preparation method of biological decomposing agent, comprising the steps of:

[0035] (1) Take a soil sample and mix it with sterile water at a mass ratio of 1:9, shake it on a shaker for 20 minutes to obtain a soil sample mixture, add the soil sample mixture to the nitrogen-fixing bacteria liquid medium at a volume ratio of 1:8, and Shake culture at 25~28℃, 100r / min for 2~3 days to obtain enriched nitrogen-fixing bacteria culture solution, repeat the enrichment culture for 3 times, obtain the re-culture enriched nitrogen-fixing bacteria culture solution, and then culture the enriched nitrogen-fixing bacteria culture solution The solution was diluted with 0.9% normal saline to 10 -5 Dilution level, to obtain the diluted nitrogen-fixing bacteria solution, take the diluted nitrogen-fixing bacteria solution and spread it on the nitrogen-fixing bacteria isolation plate medium, culture it at 25-29°C for 2-3 days, obtain nitrogen-fixing bacteria colonies, repeat the streak ...

example 1

[0040] Soil samples: taken from the rhizosphere of crops in the growing period.

[0041] Desulfurization Bacteria Screening Sample: Sludge taken from the bottom of sewage deposits.

[0042] Decomposer matrix raw materials: sawdust, rice husk, chicken manure, rice bran.

[0043] Decomposed fermented strains: EM bacteria, Pseudomonas pseudomonas, Gymboree fermented bacteria.

[0044] Desulfurization bacteria screening samples: selected from activated sludge and anaerobic sludge from sewage treatment plants.

[0045] Nitrogen-fixing bacteria liquid medium: by mass parts, take 10 parts of sucrose, K 2 HPO 4 ·3H 2 O0.2 part, NaCl0.2 part, CaCO 3 1 part, MgSO 4 ·7H 2 O 0.2 parts, yeast extract 0.5 parts, distilled water 1000 parts, pH 6.8.

[0046] Azotobacter isolation plate medium: add 20 parts of agar to the nitrogen-fixing bacteria liquid medium, and keep other components unchanged.

[0047] DF culture medium: in parts by mass, take KH 2 PO 4 4 parts, Na 2 HPO 4 6 p...

example 2

[0057]Soil samples: taken from the rhizosphere of crops in the growing period.

[0058] Desulfurization Bacteria Screening Sample: Sludge taken from the bottom of sewage deposits.

[0059] Decomposer matrix raw materials: sawdust, rice husk, chicken manure, rice bran.

[0060] Decomposed fermented strains: EM bacteria, Pseudomonas pseudomonas, Gymboree fermented bacteria.

[0061] Desulfurization bacteria screening samples: selected from activated sludge and anaerobic sludge from sewage treatment plants.

[0062] Nitrogen-fixing bacteria liquid medium: by mass parts, take 15 parts of sucrose, K 2 HPO 4 ·3H 2 O0.5 part, NaCl0.5 part, CaCO 3 2 parts, MgSO 4 ·7H 2 0.4 part, 0.8 part of yeast extract, 1000 parts of distilled water, pH 7.2.

[0063] Azotobacter isolation plate medium: add 25 parts of agar to the nitrogen-fixing bacteria liquid medium, and keep other components unchanged.

[0064] DF culture medium: in parts by mass, take KH 2 PO 4 6 parts, Na 2 HPO 4 1...

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Abstract

The invention discloses a preparation method of a biological decomposition agent and belongs to the field of agriculture. An ACC deaminase positive strain is obtained by screening plant nitrogen fixing bacteria in soil; if being applied to the soil, the ACC deaminase positive strain not only can promote the biological decomposition, but also can achieve an effect of fixing nitrogen in the soil; and the ACC deaminase positive strain directly acts on the roots of the plants, and plant growth promoting bacteria have ACC deaminase activity and can decompose ACC into ammonia and alpha-ketobutyric acid to reduce the synthesis of ethylene, so that the sensibility of the plants to an adverse situation is reduced, the stress resistance of the plants is improved, and the plant remediation of soil polluted by organic matters and soil polluted by heavy metals can be promoted. The problems that foul gas is generated in a decomposition process of the current organic material decomposition agent andthe decomposition efficiency is low are solved.

Description

technical field [0001] The invention belongs to the field of agriculture, and in particular relates to a preparation method of a biological decomposing agent. Background technique [0002] Decomposing agents refer to living microbial preparations that can accelerate the decomposition and maturity of various organic materials (including crop straw, livestock and poultry manure, domestic garbage and urban sludge, etc.). With the rapid development of my country's agriculture and processing industry, China has become the country that produces the largest amount of agricultural organic materials in the world. The agricultural organic materials produced every year are calculated in billions of tons, of which nearly 4 billion tons of livestock and poultry manure are produced every year. 700 million tons of crop straw. In recent years, the research on the resource utilization technology of agricultural waste at home and abroad has made great progress, and the resource utilization of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F17/00C05F11/08C05G3/04C05G3/80
CPCC05F11/08C05F17/20C05G3/80Y02W30/40
Inventor 黄淑枝李璐刘沁
Owner 常州文诺纺织品有限公司
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