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Alfalfa root growth-promoting rhizobacteria MJM-5 and application of alfalfa root growth-promoting rhizobacteria MJM-5

A MJM-5, a technology for promoting plant growth, applied in the field of microorganisms, which can solve the problems of agricultural and animal husbandry production loss, affecting growth and development, interfering with plant utilization and secondary metabolism, etc.

Active Publication Date: 2013-10-09
HARBIN NORMAL UNIVERSITY
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Salt-alkali stress can inhibit seed germination and seedling growth, affect the growth and development of roots, stems, leaves, flowers, fruits and other organs, inhibit the photosynthesis process of plants, affect the absorption, transport, distribution and utilization of mineral ions by roots, and interfere with the growth and development of plants. The utilization of carbon, nitrogen and oxygen and the formation of secondary metabolism also seriously affect the respiration of plants, causing serious losses to agricultural and animal husbandry production

Method used

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  • Alfalfa root growth-promoting rhizobacteria MJM-5 and application of alfalfa root growth-promoting rhizobacteria MJM-5
  • Alfalfa root growth-promoting rhizobacteria MJM-5 and application of alfalfa root growth-promoting rhizobacteria MJM-5
  • Alfalfa root growth-promoting rhizobacteria MJM-5 and application of alfalfa root growth-promoting rhizobacteria MJM-5

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1, screening and identification of alfalfa rhizosphere growth-promoting bacteria

[0057] 1. Screening

[0058] Take 1g of rhizosphere soil sample in 50mL of PAF medium, shake culture at 28°C. The PAF medium contains peptone, casein hydrolyzate, MgSO 4 , K 2 HPO 4 ,glycerin. Take 1 mL of shaken PAF culture medium, culture in 50 mL of PAF medium, shake at 28°C. Take 1 mL of the obtained PAF culture solution, and culture it with shaking at 28°C in 50 mL of DF salt culture solution. The DF salt broth contains KH 2 PO 4 , Na 2 HPO 4 , MgSO 4 , FeSO 4 , glucose, gluconic acid, citric acid, (NH 4 ) 2 SO 4 , H 3 BO 3 , MnSO 4 , ZnSO 4 , CuSO 4 , MoO 3 . Take 1 mL of the obtained DF salt culture solution, and add it to 50 mL without (NH 4 ) 2 SO 4 , but containing 3mM 1-aminocyclopropane-1-carboxylic acid (ACC) in the above-mentioned DF salt culture solution, cultured with shaking at 28°C. 1 mL of the obtained culture solution was taken, spre...

Embodiment 2

[0066] Example 2. Application of strain MJM-5 (Pseudomonas fluorescens) in the preparation of ACC deaminase

[0067] 1. Preparation of ACC deaminase by fermentation

[0068] TSB medium: tryptone 17g, soytone 3g, NaCl 5g, glucose 2.5g, K 2 HPO 4 Dissolve 2.5g in 1000ml of distilled water and adjust the pH value to pH=7.5.

[0069] ADF medium: KH 2 PO 4 4.0g, Na 2 HPO 4 6.0g, MgSO 4 ·7H 2 O0.2g, FeSO 4 ·7H 2 O0.1g, glucose 2.0g, gluconic acid 2.0g, citric acid 2.0g, trace elements 0.1mL, after adding distilled water to dissolve, adjust the pH value to pH=7.5, set the volume to 1000ml, add 1-amino Cyclopropane-1-carboxylic acid (ACC), so that the final concentration is 3.0mmol / L (you can first prepare the mother liquor 0.5mol / L); among them, trace elements (100mL): H 3 BO 3 10mg, MnSO 4 11.2mg, ZnSO 4 124.6mg, CuSO 4 78.2mg, MoO 3 10 mg.

[0070] None (NH 4 ) 2 SO 4 DF medium: KH 2 PO 4 4.0g, Na 2 HPO 4 6.0g, MgSO 4 ·7H 2 O0.2g, FeSO 4 ·7H 2O0.1g, gluc...

Embodiment 3

[0078] Example 3, the application of strain MJM-5 (Pseudomonas fluorescens) in the preparation of IAA synthesis

[0079] 1. Preparation of IAA

[0080] DF medium: KH 2 PO 4 4.0g, Na 2 HPO 4 6.0g, MgSO 4 ·7H 2 O0.2g, FeSO 4 ·7H 2 O0.1g, glucose 2.0g, gluconic acid 2.0g, citric acid 2.0g, (NH 4 ) 2 SO 4 2.0g, 0.1mL of trace elements, dissolved in distilled water, adjust the pH value to pH=7.5, and dilute to 1000ml. The preparation of trace elements is the same as above.

[0081] The tested strain MJM-5 (Pseudomonas fluorescens) was cultured in DF medium at 28°C and 180rpm for 2 days, and then transferred to different concentrations (0, 50, 100, 200, 500 μg L-Trp·mL -1 ) tryptophan (L-Trp) in fresh DF medium, cultured at 28° C., 180 rpm for 2 days, and collected the fermentation product. Sampling to measure the OD of the bacterial solution 600 Then centrifuge the rest of the fermentation product (bacteria liquid) at room temperature (25°C) at 8000rpm for 10min, coll...

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Abstract

The invention discloses a strain of alfalfa root growth-promoting rhizobacteria MJM-5 and application of the alfalfa root growth-promoting rhizobacteria MJM-5. A pseudomonas fluorescens strain MJM-5 provided by the invention has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6293. The pseudomonas fluorescens strain MJM-5 has at least one kind of application shown in 1) to 4): 1) ACC (aminocyclopropane carboxylic acid) deaminase preparation; 2) IAA (indole acetic acid) preparation; 3) siderophores preparation; and 4) plant growth promotion under saline alkali stress. Experiments prove that the strain of alfalfa root growth-promoting rhizobacteria MJM-5 obtained through separation can be used for synthesizing ACC deaminase and IAA siderophores, and in addition, the alfalfa root growth-promoting rhizobacteria MJM-5 has the effects of effectively promoting the plant nutrition absorption, regulating the plant growth and improving the stress resistance capability of plants under the stress conditions in the saline alkali stress environment.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an alfalfa rhizosphere growth-promoting bacterium MJM-5 and its application. Background technique [0002] Saline-alkali soil is a soil type widely distributed on land, and it is an ecologically fragile area on the land surface. At present, the area of ​​saline-alkali land in the world is more than 1 billion hectares, and with the deterioration of the ecological environment, the secondary salinization of the soil is also increasing, which affects the development of agriculture and animal husbandry, aggravates the degree of desertification and desertification, and has become a global environmental problem. question. There are about 12 million hectares of saline-alkali land in China, accounting for about 1.2% of the world's saline-alkali land area and about 15% of China's existing cultivated land area. The reduction of vegetation in these areas has become an important facto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/88A01N63/02A01P21/00C12P17/10C12P1/04C12R1/39
Inventor 郭长虹马嘉敏王晓丹蔡洪生谢宝明
Owner HARBIN NORMAL UNIVERSITY
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