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Endogenous growth-promoting rhizobium of rices and application thereof

A rice rhizobium, microbial strain technology, applied in applications, bacteria, biocides, etc.

Active Publication Date: 2018-08-14
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the bottleneck restricting the development of my country's microbial fertilizer industry is the selection of high-efficiency strains

Method used

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  • Endogenous growth-promoting rhizobium of rices and application thereof
  • Endogenous growth-promoting rhizobium of rices and application thereof
  • Endogenous growth-promoting rhizobium of rices and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1. Isolation and identification of Rhizobium oryzihabitans M15

[0051] 1 Isolation of Rhizobium oryzihabitans M15

[0052] Rhizobium oryzihabitans M15 was isolated from rice roots, and the rice variety was 90-3 (Oryza sativa L.japonica 90-3). The sampling location was the 100-acre agricultural experiment demonstration base in Sangyuan Town, Luannan County, Hebei Province. Separation process: The rice root surface was sterilized by ethanol-sodium hypochlorite, and after gradient dilution, the R 2 A was isolated by coating on solid medium, cultured at 28°C for 48 hours, picked a single colony on the plate and streaked to purify to obtain a purified strain, and stored on the slanted surface in a refrigerator at 4°C. One of the isolated and purified endophytic bacteria was numbered M15, hereinafter referred to as endophytic bacteria M15.

[0053] Identification of Rhizobium oryzihabitans M15

[0054] 2.1 Morphological identification

[0055] Describe the colony...

Embodiment 2

[0075] Embodiment 2, the physiological and biochemical characteristics of rice rhizobium (Rhizobium oryzihabitans) M15

[0076] 1.1 Determination of salt tolerance: Inoculate the same concentration of Rhizobium oryzihabitans M15 bacterial liquid in YMA liquid medium containing 0%, 1.0%, 3.0%, 5.0%, 7.0%, 8.0%, 10.0% NaCl (1% inoculum size, v / v), shake culture at 30°C for 7 days, measure the absorbance value at 600nm with a UV-1800 ultraviolet spectrophotometer, and find that Rhizobium oryzihabitans M15 can still survive at a salinity of 7.0%. growth (weak growth), and the optimum growth salt concentration range is 0-3%.

[0077] 1.2 pH range measurement: prepare phosphate buffer solution (solution A: 8.3mL phosphoric acid, add deionized water, mix and set the volume to 500mL; solution B: Na 2 HPO 4 14.326g, add deionized water and mix to 500mL; take 72.5mL of A solution and 27.5mL of B solution and mix evenly, pH is about 2.0), acetic acid-sodium acetate buffer system (0.2m...

Embodiment 3

[0081] Example 3, Qualitative detection and quantitative analysis of auxin secreted by Rhizobium oryzihabitans M15

[0082]According to reference (Eric Glickm ann, Yves Dessaux.A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria[J].Applied and Environmental mental Microbiology, 1995(2):793-796) described Salkowski colorimetry was used to determine the performance of auxin (IAA) secretion from Rhizobium oryzihabitans M15. Rhizobium oryzihabitans M15 was inoculated in R 2 A liquid culture medium was cultured on a shaker at 28° C. and shaken at 180 rpm for 4 days to obtain a fermentation broth of Rhizobium oryzihabitans M15. uninoculated R 2 A liquid medium is used as a blank control to measure the OD600nm value of the rice rhizobium (Rhizobium oryzihabitans) M15 fermentation liquid, and the result shows that the OD600nm value of this rice rhizobium (Rhizobium oryzihabitans) M15 fermentation liquid is 1.5...

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Abstract

The invention discloses endogenous growth-promoting rhizobium of rices and application thereof. The endogenous growth-promoting rhizobium of the rices is rhizobium oryzihabitans, the strain number isM15, and the registration number of China General Microbiological Center of Microbiological Culture Collection Committee is CGMCC No.15530. Proved by experiments, rhizobium oryzihabitans M15 can generate IAA, every liter of fermenting liquid with the OD600nm value being 1 contains 85.32mu g IAA. The rhizobium oryzihabitans M15 can generate ACC deaminase, the activity of the ACC deaminase of the rhizobium oryzihabitans M15 is 4.86mu mol alpha-butanone acid / h.mg protein.

Description

technical field [0001] The invention relates to a rice endogenous growth-promoting rhizobia in the field of microbial fertilizers and its application. Background technique [0002] Rhizobia are a class of Gram-negative bacteria widely present in soil, which can infect the roots or stems of legumes to form root nodules or stem nodules, and fix N in the air in a symbiotic manner. 2 Provide plants with NH that can be absorbed and utilized 4 + . In agricultural production, crop rotation and intercropping between rhizobia and leguminous crops can improve soil fertility and play a vital role in increasing crop yield. In addition, because rhizobia can grow and reproduce in large quantities in the roots of crops, the chance of pathogenic microorganisms to reproduce is reduced; at the same time, rhizobia can also induce plant systemic resistance, reduce the probability of crop disease, and improve its disease resistance. After soybeans are inoculated with rhizobia, it can reduce ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01N43/38A01P21/00C12R1/41
CPCA01N43/38A01N63/00A01N63/10C12N1/20C12N1/205C12R2001/41
Inventor 张晓霞赵娟娟赵霞张桂山马晓彤郭鹤宝
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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