Preparation method of phosphate-solubilizing microbial agent and application thereof

A technology of microbial inoculum and phosphorus-dissolving bacteria, which is applied in the field of microorganisms, can solve the problems of insufficient soil organic matter, reduce insoluble phosphorus content, secondary environmental damage, etc., and achieve the effects of simple application method, improvement of fertility status, and promotion of growth.

Inactive Publication Date: 2018-10-23
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Using chemical methods to reduce the content of insoluble phosphorus will cause secondary damage to the environment
Farmers ...

Method used

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  • Preparation method of phosphate-solubilizing microbial agent and application thereof
  • Preparation method of phosphate-solubilizing microbial agent and application thereof
  • Preparation method of phosphate-solubilizing microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Example 1: Phosphorus-dissolving ability of phosphorus-dissolving microbial bacterial agent

[0032] The phosphate-soluble microorganism Aspergillus aculeatus was inoculated into a solid medium containing 0.5% insoluble inorganic phosphorus by the spot method, and cultured upside down at 30°C for 7 days to observe the change of colonies in the medium. The result is as figure 1 As shown, a white lysis zone appeared around the colony, indicating that the phosphorus-soluble microorganism Aspergillus aculeatus fungus can dissolve insoluble phosphorus.

[0033] The bacterium solution of the phosphorus-dissolving microorganism Aspergillus aculeatus fungus (the number of bacteria in the bacterium solution is 1×10 9 ~9×10 9 CFU / mL range), with 3% inoculum amount into tricalcium phosphate liquid medium, cultivated in Erlenmeyer flask, using molybdenum antimony anti-colorimetric method to measure the concentration of soluble phosphorus in the fermentation broth, the results...

Embodiment 2

[0034] Embodiment 2: the ability of phosphate-dissolving microbial bacterial agent to secrete organic acid

[0035] The principle of the phosphorus-soluble microorganism Aspergillus aculeatus fungus to dissolve inorganic phosphorus is to secrete organic acids, so as to dissolve the insoluble inorganic phosphorus in the soil into soluble available phosphorus for plant absorption and utilization; using tricalcium phosphate liquid medium, through high-efficiency liquid phase Chromatography was used to determine the type and concentration of organic acids in the fermentation broth. The results are shown in Table 1. In the tricalcium phosphate liquid medium added to fungi, the content of organic acids increased with the prolongation of the culture time. The main organic acids Acid species include: tartaric acid, citric acid, and glycine, thus demonstrating that the mechanism by which fungi dissolve tricalcium phosphate is through the secretion of organic acids.

[0036] Table 1 The...

example 3

[0038] Example 3: Preparation of Phosphorus-Solubilizing Microbial Inoculum and Its Use in Pot Experiment of Ryegrass

[0039] The bacterial liquid of Aspergillus aculeatus fungus (the number of bacteria in the bacterial liquid is 1×10 9 ~9×10 9 within the range of CFU / mL), with 3% inoculum amount into 100mL Martin’s (Martin) liquid medium, 30°C, 170r / min shaking culture for 48h, the liquid phosphate-dissolving bacteria solution was obtained; 20mL of the above-mentioned liquid phosphorus-dissolving bacteria Add 100g of sand and peat to the solid medium (v / v=2:1), adjust the pH to 6, and incubate at 30°C for 3 days to make a solid phosphorus-dissolving microbial bacterial agent.

[0040] The ryegrass pot experiment set up a treatment group and a control group. The treatment group was treated with 5g / pot of the above-mentioned solid microbial agent (500g of phosphorus-deficient and insoluble phosphorus soil in each pot), and the control group was treated with inactivation at 12...

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Abstract

The invention discloses a preparation method of a phosphate-solubilizing microbial agent and application thereof and belongs to the field of microbial technology. The preparation method of the phosphate-solubilizing microbial agent comprises the following steps: (1) preparation of a liquid state phosphate-solubilizing microbial solution; and (2) preparation of a solid state phosphate-solubilizingmicrobial agent. The application of the solid state phosphate-solubilizing microbial agent comprises the following steps: (1) during building and planting, the solid state phosphate-solubilizing microbial agent is applied by a broadcasting mode, and the application amount is 10-20 kg/Mu; and (2) during sowing, the solid state phosphate-solubilizing microbial agent and seeds undergo seed dressing (1 kg of ryegrass seeds are stirred into 5-8 kg of the solid state phosphate-solubilizing microbial agent), and sowing is conducted. The phosphate-solubilizing microorganism, namely Aspergillus aculeatus has endophilicity with ryegrass, has capabilities of secreting IAA, siderophore, ACC deaminase and organic acid, can effectively promote plant growth while solubilizing phosphate, can improve soilfertility, and can greatly increase content of rapidly available phosphorus in soil. The preparation is convenient, application method is simple, and a broadcasting or seed dressing method, etc. can be adopted for inoculation; and cost is low, and operation is simple.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation method and application of a phosphorus-dissolving microbial bacterial agent. Background technique [0002] Phosphorus is an indispensable element in the formation of nuclear protein, lecithin and so on. Phosphorus can accelerate cell division, accelerate root and shoot growth, promote flower bud differentiation, early maturity, and improve fruit quality. [0003] my country has 550 million mu of various saline-alkali land resources available, and 33.7 million mu of severe saline-alkali land, which is the largest reserve cultivated land resource. Any nutrients in chemical fertilizers such as nitrogen and phosphorus that are not absorbed by plants or absorbed and fixed by the root layer soil will accumulate below the root layer or transfer to groundwater, becoming potential environmental pollutants. Saline-alkali land has a high PH value and high ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N11/14A01N63/04A01P21/00A01C1/06A01G7/06A01B79/02C12R1/66
CPCA01B79/02A01C1/06A01G7/06A01N63/30C12N1/14C12N11/14
Inventor 傅金民李晓宁谢燕范树高王伟王广阳张寅坤李佳南孙志超王誉璠
Owner LUDONG UNIVERSITY
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