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A rice endophytic nitrogen-fixing bacterium that produces acc deaminase and antagonizes scab and its use

A deaminase, pathogenic fungus technology, applied in the direction of enzymes, applications, bacteria, etc., to achieve the effects of good inoculation effect, broad application prospects and strong competitive adaptability

Inactive Publication Date: 2011-12-21
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the bottleneck restricting the development of my country's microbial fertilizer industry is the selection of high-efficiency strains

Method used

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  • A rice endophytic nitrogen-fixing bacterium that produces acc deaminase and antagonizes scab and its use
  • A rice endophytic nitrogen-fixing bacterium that produces acc deaminase and antagonizes scab and its use
  • A rice endophytic nitrogen-fixing bacterium that produces acc deaminase and antagonizes scab and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1, Isolation and identification of rice endogenous nitrogen-fixing bacteria GDSD125

[0050] 1. Isolation of rice endophytic nitrogen-fixing bacteria

[0051] The specific operation for the isolation of endogenous nitrogen-fixing bacteria in rice is as follows: take fresh rice plants (collected from Heilongjiang Province, China), first rinse them with tap water, then soak them in 70% ethanol for 1 min, sterilize the surface with 2% sodium hypochlorite for 10 min, and sterile Rinse with water 3 times. Under aseptic operation conditions, accurately weigh 10.0g of the sample, grind it into a paste in a sterile mortar, transfer and set the volume to 100ml, and continue to dilute to make serial dilution samples, respectively starting from 10 -4 、10 -5 、10 -6 Take 0.1ml of the diluted solution and spread evenly on the above-mentioned CCM medium and nitrogen-free medium plate respectively, culture upside down at 28°C, and after 3-4 days, pick a single colony and str...

Embodiment 2

[0074] Example 2, Determination of Nitrogenase Activity of Burkholderia sp. GDSD125 CGMCC No.5038

[0075] Carry out nitrogenase activity assay to Burkholderia sp. (Burkholderia sp.) GDSD125 CGMCC No.5038 obtained in embodiment 1, specific method is as follows: in 15 * 150mm screw-top glass tubes, add 5ml to improve nitrogen fixation culture The base was made into a slant, inoculated with nitrogen-fixing bacteria, and cultured at 28°C. Azotobacter chroococcum (Azotobacter chroococcum) ACCC11103, which is commonly used in the production of microbial fertilizers, was used as the positive control, and the blank slant without inoculation was used as the negative control, with 3 replicates. After culturing for 72 hours, replace the rubber stopper, inject acetylene gas to make the final concentration 10%, seal it with medical adhesive tape, continue culturing for 72 hours, take 100 μl of reaction gas, measure the amount of ethylene produced by gas chromatography, and calculate the n...

Embodiment 3

[0078] Embodiment 3, Burkholderia sp. (Burkholderia sp.) GDSD125 CGMCC No.5038 antagonizes pathogenic fungus inhibition rate determination

[0079] The Burkholderia sp. (Burkholderia sp.) GDSD125 CGMCC No.5038 obtained in embodiment 1 is adopted to measure the antibacterial rate of antagonizing pathogenic fungi by two-point confrontation method, and the specific operations are as follows: The crop pathogenic fungi Gibberella zeae (Gibberella zeae) and Burkholderia sp. (Burkholderia sp.) GDSD125 CGMCC No.5038 were inoculated on two points of 2 cm respectively, and each screening treatment was repeated 3 times to only inoculate the pathogenic fungi but not The plate inoculated with Burkholderia sp. GDSD125 CGMCC No.5038 was used as a control. Incubate at a constant temperature at 28°C, and measure the colony radius r of the pathogenic fungi on the confrontation plate along the direction of the tested Burkholderia sp. GDSD125 CGMCC No.5038 with a millimeter scale after 15 days 1...

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Abstract

The invention discloses a rice endotrophic azotobacter for producing activated calcium carbonate (ACC) and realizing the antagonistic effect on fusarium graminearum and a purpose thereof. The rice endotrophic azotobacter is Burkholderia sp. GDSD125 and has the preservation number being CGMCC No.5038 in General Microbiology Center of China Microbial Strain Preservation Management Committee. The Burkholderia sp. GDSD125 CGMCC No.5038 provided by the invention has high nitrogen fixation activity and ACC ammonialyase activity, can realize the effective antagonistic effect on gibberella zeae and has wide application prospects in nitrogen fixation microbial agent and microbial organic fertilizer production.

Description

technical field [0001] The present invention relates to a rice endogenous nitrogen-fixing bacterium that produces ACC deaminase and antagonizes head blight and uses thereof, in particular relates to a strain that can produce ACC deaminase, antagonize crop head blight, and has high-efficiency nitrogen fixation in rice Functional bacteria. Background technique [0002] Nitrogen fertilizer is one of the most important means of production in agricultural production. The production of chemical nitrogen fertilizer consumes a large amount of energy, accounting for 70% to 80% of the total production cost. In recent years, energy has become increasingly tense, and the price of coal for chemical fertilizer production in my country has soared, leading to an increase in the price of chemical nitrogen fertilizers, which is unbearable for farmers and has a strong response. In addition, chemical nitrogen fertilizers have played a huge role in increasing crop yields and ensuring food secu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/88C12N9/00A01N63/02A01P3/00A01P21/00C05F11/08C12R1/01
Inventor 孙建光徐晶高淼胡海燕
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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