Microorganism microbial agent for promoting growth of rice and reducing cadmium and preparation method and use method of microorganism microbial agent
A microbial agent and rice technology, applied in the field of microbial treatment of heavy metal pollution, can solve the problems of unsuitable for large-scale treatment, limited repair depth, long cycle, etc., and achieve the effects of low production cost, reduced absorption, and simple operation
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[0032] The formula of the liquid culture medium of Listeria gasseri is: agar 2.5g / L, gelatin 80g / L, glucose 10g / L, sterilized defibrinated sheep blood or rabbit blood 6g / L to adjust the pH to 7.2-9.6;
[0033] The formula of the liquid culture medium of Burkholderia is: mannitol 10g / L; ammonium sulfate 2g / L; peptone 2g / L; dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L, sodium chloride 0.1g / L; ferrous sulfate 0.05g / L; manganese sulfate 0.05g / L, adjust the pH value to 7.0;
[0034] The liquid medium formula of Pseudomonas stutzeri is: tryptone 15g / L, soytone 5.0g / L, sodium chloride 5.0g / L, agar 13g / L, adjust the pH to 7.0;
[0035] The formula of the liquid culture medium of Micromonas aculeatus is: peptone 10g / L, yeast powder 5g / L, sodium chloride 10g / L, adjust the pH to 7.0;
[0036] The formula of the liquid culture medium of Bacillus subtilis is: beef extract 10g / L; agar 2g / L; peptone 2g / L; dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L, s...
preparation example 1
[0038] A microbial agent for promoting rice growth and reducing cadmium, comprising the following components in terms of quantity percentage: 50% of Listeria grizzi, 20% of Burkholderia, 10% of Pseudomonas stutzeri, and Echinococcus sp. Micromonospora 10%, Bacillus subtilis 20%.
[0039]A preparation method of a microbial bacterial agent for promoting rice growth and reducing cadmium is: strains of Listeria grizzi, Burkholderia, Pseudomonas stutzeri, Micromonospora aculeatus, and Bacillus subtilis After the slant surface was activated, they were inoculated in liquid medium for extended cultivation for 48 hours, and then the fermentation medium was added, with glucose as the carbon source, beef extract as the nitrogen source, and calcium chloride as the inorganic salt to adjust the pH value of the medium. Cultivate at 30°C and 170r / min for 36 hours. The fermentation medium includes glucose 15g / L, beef extract 3.0g / L, and calcium chloride 1.0g / L. Adjust the pH value of the mediu...
preparation example 2
[0041] A microbial bacterial agent for promoting rice growth and reducing cadmium, comprising the following components in terms of quantity percentage: 46% of Listeria grizzi, 25% of Burkholderia, 6% of Pseudomonas stutzeri, and Echinococcus sp. Micromonospora 12%, Bacillus subtilis 11%.
[0042] A preparation method of a microbial bacterial agent for promoting rice growth and reducing cadmium is: strains of Listeria grizzi, Burkholderia, Pseudomonas stutzeri, Micromonospora aculeatus, and Bacillus subtilis After the slant surface was activated, they were inoculated in liquid medium for extended cultivation for 60 h, and then added to fermentation medium, with glucose as carbon source, beef extract as nitrogen source, and calcium chloride as inorganic salt to adjust the pH value of the medium. Cultivate at 30°C and 170r / min for 48 hours. The fermentation medium includes glucose 20g / L, beef extract 4.0g / L, and calcium chloride 2.0g / L. Adjust the pH value of the medium to 6.5, a...
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