Microorganism microbial agent for promoting growth of rice and reducing cadmium and preparation method and use method of microorganism microbial agent

A microbial agent and rice technology, applied in the field of microbial treatment of heavy metal pollution, can solve the problems of unsuitable for large-scale treatment, limited repair depth, long cycle, etc., and achieve the effects of low production cost, reduced absorption, and simple operation

Pending Publication Date: 2019-11-19
HUNAN AGRICULTURAL UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional soil heavy metal remediation methods are limited due to shortcomings such as long cycle, limited remediation depth, and unsuita

Method used

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  • Microorganism microbial agent for promoting growth of rice and reducing cadmium and preparation method and use method of microorganism microbial agent
  • Microorganism microbial agent for promoting growth of rice and reducing cadmium and preparation method and use method of microorganism microbial agent
  • Microorganism microbial agent for promoting growth of rice and reducing cadmium and preparation method and use method of microorganism microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] The formula of the liquid culture medium of Listeria gasseri is: agar 2.5g / L, gelatin 80g / L, glucose 10g / L, sterilized defibrinated sheep blood or rabbit blood 6g / L to adjust the pH to 7.2-9.6;

[0033] The formula of the liquid culture medium of Burkholderia is: mannitol 10g / L; ammonium sulfate 2g / L; peptone 2g / L; dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L, sodium chloride 0.1g / L; ferrous sulfate 0.05g / L; manganese sulfate 0.05g / L, adjust the pH value to 7.0;

[0034] The liquid medium formula of Pseudomonas stutzeri is: tryptone 15g / L, soytone 5.0g / L, sodium chloride 5.0g / L, agar 13g / L, adjust the pH to 7.0;

[0035] The formula of the liquid culture medium of Micromonas aculeatus is: peptone 10g / L, yeast powder 5g / L, sodium chloride 10g / L, adjust the pH to 7.0;

[0036] The formula of the liquid culture medium of Bacillus subtilis is: beef extract 10g / L; agar 2g / L; peptone 2g / L; dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L, s...

preparation example 1

[0038] A microbial agent for promoting rice growth and reducing cadmium, comprising the following components in terms of quantity percentage: 50% of Listeria grizzi, 20% of Burkholderia, 10% of Pseudomonas stutzeri, and Echinococcus sp. Micromonospora 10%, Bacillus subtilis 20%.

[0039]A preparation method of a microbial bacterial agent for promoting rice growth and reducing cadmium is: strains of Listeria grizzi, Burkholderia, Pseudomonas stutzeri, Micromonospora aculeatus, and Bacillus subtilis After the slant surface was activated, they were inoculated in liquid medium for extended cultivation for 48 hours, and then the fermentation medium was added, with glucose as the carbon source, beef extract as the nitrogen source, and calcium chloride as the inorganic salt to adjust the pH value of the medium. Cultivate at 30°C and 170r / min for 36 hours. The fermentation medium includes glucose 15g / L, beef extract 3.0g / L, and calcium chloride 1.0g / L. Adjust the pH value of the mediu...

preparation example 2

[0041] A microbial bacterial agent for promoting rice growth and reducing cadmium, comprising the following components in terms of quantity percentage: 46% of Listeria grizzi, 25% of Burkholderia, 6% of Pseudomonas stutzeri, and Echinococcus sp. Micromonospora 12%, Bacillus subtilis 11%.

[0042] A preparation method of a microbial bacterial agent for promoting rice growth and reducing cadmium is: strains of Listeria grizzi, Burkholderia, Pseudomonas stutzeri, Micromonospora aculeatus, and Bacillus subtilis After the slant surface was activated, they were inoculated in liquid medium for extended cultivation for 60 h, and then added to fermentation medium, with glucose as carbon source, beef extract as nitrogen source, and calcium chloride as inorganic salt to adjust the pH value of the medium. Cultivate at 30°C and 170r / min for 48 hours. The fermentation medium includes glucose 20g / L, beef extract 4.0g / L, and calcium chloride 2.0g / L. Adjust the pH value of the medium to 6.5, a...

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Abstract

The invention discloses a microorganism microbial agent for promoting growth of rice and reducing cadmium and a preparation method and use method of the microorganism microbial agent, and belongs to the technical field of government of heavy metal pollution with microorganisms. The microorganism microbial agent provided by the invention comprises listeria grayi, burkholderia sp., pseudomonas stutzeri, bacillus subtilis and micromonospora echinospora, and is prepared through the steps of performing strain activation, performing enlarged culture, performing fermentation culture and performing mixing. At the growth resuming period during growth of the rice, the microorganism microbial agent is added to rice water culture potting nutrient fluid in the volume ratio of 1 to 400, and culturing isperformed until the highest tillering stage is achieved. Under the condition of the percentage by quantity of the components of the microorganism microbial agent that the listeria grayi is 50%, the burkholderia sp. is 20%, the pseudomonas stutzeri is 10%, the micromonospora echinospora is 10% and the bacillus subtilis is 20%, the absorptivity of the heavy metal cadmium by the rice is the lowest,and the potential of promoting growth of the rice is achieved. The microorganism microbial agent prepared by the preparation method disclosed by the invention has positive influence on promoting growth of the rice and reducing the absorption of the cadmium by the rice, and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment of heavy metal pollution, and in particular relates to a microbial bacterial agent for promoting rice growth and reducing cadmium, as well as a preparation method and an application method thereof. Background technique [0002] Rice is the main food crop in my country, and it is also a crop with a very high cadmium enrichment index. According to incomplete statistics, the area of ​​farmland polluted by cadmium in my country has exceeded 200,000 hectares, and the annual production of agricultural products with excessive cadmium content reaches 1.46 billion kilograms. Due to heavy metal pollution, grain production is reduced by more than 10 million tons every year, and more than 1,200 contaminated grains are polluted. 10,000 tons, and the economic loss amounted to more than 20 billion yuan. Cadmium is very mobile, and it is easy to accumulate in plants through the absorption of plant roo...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10A01N63/00A01N25/32A01P21/00C12R1/01C12R1/38C12R1/125C12R1/29
CPCC12N1/20B09C1/10A01N63/00A01N25/32
Inventor 黎娟尹华群黎鹏郑重谊周婕怡黎俊
Owner HUNAN AGRICULTURAL UNIV
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