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Burkholderia and application method thereof

A kind of Holderella, application method technology, applied in microbial strain and its application field

Inactive Publication Date: 2015-05-20
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, reports on the leaching of heavy metal-contaminated soils by Burkholderia sp.

Method used

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  • Burkholderia and application method thereof
  • Burkholderia and application method thereof
  • Burkholderia and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: bacterial strain biological identification

[0022] Pick a single colony and inoculate it in the improved LB liquid medium, culture it overnight in a constant temperature shaking incubator at 35°C, collect the bacteria by centrifugation, extract the DNA of the bacteria according to the operation of the bacterial DNA extraction kit of TIANGEN company, and perform PCR amplification . The PCR amplification product was sequenced by a sequencing company, and the 16S rDNA was compared with the registered 16S rDNA sequence in Genbank. It was 100% similar to the 16s rDNA of Burkhoideria sp.IHB B 2259 and Burkhoideria sp.SBH-14, The strain was named Burkhoideria sp.Z-90, and the scanning electron micrograph and phylogenetic analysis tree of the strain are shown in figure 2 , image 3 .

Embodiment 2

[0023] Example 2: Determination of the type of biosurfactant produced by Burkhoideria sp.Z-90

[0024] The types of biosurfactants produced by Burkhoideria sp.Z-90 were determined by thin-layer chromatography and infrared spectroscopy. The specific experimental conditions are as follows: filter the fermented liquid after five days of cultivation on a 0.22 micron filter membrane, centrifuge the filtrate for 20 min at a speed of 8000 r / min, filter the supernatant again, and adjust the concentration of the filtrate with 6 mol / L HCl solution. When the pH value reaches 2, add an equal volume of ethyl acetate solution to the filtrate and stir on a magnetic stirrer to fully mix the filtrate and the extractant, repeat twice, combine the organic phases, and distill under reduced pressure at 45°C until The solution in the round-bottomed flask was a yellow-brown viscous liquid, poured out, and dried in a vacuum oven at 35°C. After the organic phase was completely volatilized, the surfact...

Embodiment 3

[0025] Example 3: Restoration of contaminated soil in a smelter factory area in Hengyang, Hunan

[0026]The collected polluted soil from a smelter in Hengyang, Hunan was air-dried, ground, passed through a 4.75mm aperture sieve, and 12.5g was taken and sterilized in a sterilizing pot at 150°C for 30min. Take 247.5 mL of prepared modified LB liquid medium, put it into a 500 mL Erlenmeyer flask, and sterilize it in a sterilizing pot at 121 °C for 30 min. After sterilization and cooling, inoculate 2.5mL OD 620 The inoculum with a value of about 3.6 was cultured in a sterilized modified LB liquid medium in a constant temperature shaking incubator at 35°C for two days to obtain a bacterial solution. Add 12.5g of sterilized soil into the bacterial liquid, put it back into the constant temperature shaking incubator, take it out and filter it after five days, after filtering, the soil is air-dried and ground, microwave digested, and the heavy metal concentration in the digested liqui...

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Abstract

The invention discloses burkholderia and an application method thereof. The preservation number of a strain Burkholderia sp. Z-90 is CGMCC No.9970. The strain can produce a large amount of glycolipid biosurfactants in the metabolic process, and the biosurfactants can complex and desorb heavy metals in contaminated soil. The strain is used for treating soil contaminated by heavy metals, the contents of heavy metals such as Zn, Pb, Mn, Cd, Cu and As in the soil are respectively reduced from original 22374.1 mg / kg, 2472.4 mg / kg, 1837.2 mg / kg, 447.0 mg / kg, 709.5 mg / kg and 562.2 mg / kg to 12525.4 mg / kg, 1670.0 mg / kg, 878.1 mg / kg, 278.3 mg / kg, 538.6 mg / kg and 384.3 mg / kg, and the removal rates are respectively 44.0%, 32.5%, 52.2%, 37.7%, 24.1% and 31.6%.

Description

technical field [0001] The invention relates to the technical field of microbial strains and applications thereof, in particular to a strain of Burkholderia and an application method thereof. Background technique [0002] With the development of industry, a large amount of heavy metal elements enter the soil, seriously endangering the growth of animals and plants, and further affecting terrestrial species and humans through processes such as bio-accumulation and food chain amplification, which has a major impact on national security. In recent years, heavy metal-contaminated soil remediation methods have been extensively studied. The commonly used remediation methods for heavy metal contaminated soil mainly include physical remediation, chemical remediation and biological remediation. Physical remediation requires a large amount of work, consumable materials and labor, and it is difficult to treat heavy metal pollution in the soil to the required value; chemical remediation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 杨志辉柴立元张志肖睿洋杨卫春李青竹王庆伟唐崇俭廖骐
Owner CENT SOUTH UNIV
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