Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Burkholderia FH4 bacterial strain, and screening method and application thereof

A technology of Burkholderia and screening methods, applied in the field of microorganisms, can solve the problems of high cost, unsuitable for large-scale industrial production, environmental pollution, etc., and achieve the effects of low production cost, easy operation and simple equipment

Inactive Publication Date: 2014-05-28
SHANGHAI OCEAN UNIV
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cellulose in Enteromorpha is the main component used to produce bioethanol. Commercial cellulase is generally used to degrade the cellulose in Enteromorpha. However, the cost of commercial cellulase is relatively high, and the enzymatic hydrolysis process of cellulose Pollution to the environment, not suitable for large-scale industrial production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Burkholderia FH4 bacterial strain, and screening method and application thereof
  • Burkholderia FH4 bacterial strain, and screening method and application thereof
  • Burkholderia FH4 bacterial strain, and screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Screening of Burkholderia strain FH4

[0031](1) The samples were collected from the rotten proliferative algae that grew in natural conditions and densely floating in the green tide sea area. After collection, they were transported back to the laboratory at low temperature in an incubator for bacterial screening.

[0032] Add 1 mL of the collected algal suspension of Enterobacter rota to a conical flask containing 30 mL of selective medium, fix the conical flask on a shaker, and shake at 30°C and 140 r / min for 2 to 3 days, until the culture The liquid becomes cloudy.

[0033] Among them, the selection medium is: cellulose powder 5g,

[0034] Sodium nitrate 1g,

[0035] Potassium chloride 0.5g,

[0036] Disodium hydrogen phosphate 1.2g,

[0037] Potassium dihydrogen phosphate 0.9g,

[0038] Magnesium sulfate 0.5g,

[0039] Yeast paste 0.5g,

[0040] Hydrolyzed casein 0.5g,

[0041] Chen sea water 1000mL.

[0042] (2) Dilute the medium after selective culture: ...

Embodiment 2

[0051] Preparation of crude enzyme solution.

[0052] (1) Burkholderia FH4 obtained in Example 1 was cultured in LB liquid medium at 28° C. and 140 r / min for 24 hours, and then a freezing buffer was added to the bacterial liquid. The volume concentration of the buffer is 50% glycerol, the volume ratio of the buffer to the bacterial solution is 1:1, and the freezing buffer is added. In order to better protect the Burkholderia FH4 strain, the strain is not will die from dehydration.

[0053] (2) The above-mentioned Burkholderia FH4 strain was activated and cultured on a slant in LB medium, and after 2 generations of activation, a full ring was picked and placed in LB liquid medium, and cultured at 28°C and 140r / min for 24h. Take 1 mL in 50 mL of sterilized and cooled cellulose liquid fermentation medium, and shake at 35°C and 140 r / min for 6 days.

[0054] The liquid fermentation medium is:

[0055] Yeast extract 1g,

[0056] Peptone 5g,

[0057] Sodium carboxymethyl cellul...

Embodiment 3

[0064] Preparation of crude enzyme solution.

[0065] Step (1) and step (2) are the same as in Example 2.

[0066] (3) Filter the liquid fermentation culture liquid obtained in step (2), and take the filtered solution as crude enzyme liquid.

[0067] Activity detection of crude enzyme solution:

[0068] Filter paper enzymatic activity (FPA) assay: the assay method is the same as that in Example 2, and the measured enzymatic activity is 22.28 U / mL.

[0069] CMC enzyme activity assay: the assay method is the same as that in Example 2, and the measured enzyme activity is 45.77 U / mL.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of microorganism, and particularly relates to a Burkholderia FH4 bacterial strain, and a screening method and application thereof. The preservation number of the Burkholderia FH4 bacterial strain is CGMCC No.8271; the application method of enzymolysis Enteromorpha of the bacterial strain comprises the steps: performing excitation culture on a cryopreserved FH4 bacterial strain in an LB slant culture medium, then selecting and putting a complete ring into an LB culture liquid medium, performing shaking culture for 24 to 28 h, putting 1 to 2 mL of a bacterial solution into cellulose liquid fermentation culture medium subjected to sterilization, performing shaking culture for 5 to 7 days, then performing low-temperature centrifugation on fermentation liquor, using supernate as crude enzyme, or filtering the fermentation liquor, and using filter liquor as crude enzyme. The crude enzyme reacts with clean Enteromorpha powder at 45 to 55 DEG C. The Burkholderia FH4 bacterial strain has the capacity of producing cellulose, can effectively degrade cellulose substance in Enteromorpha into reducing sugar, and is low in production cost, simple in equipment, mild in reaction and applicable to large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and particularly relates to a Burkholderia FH4 strain and a screening method and application thereof. Background technique [0002] At present, the supply of oil resources in the world is becoming more and more tense, the environmental pollution is becoming more and more serious, and the energy problem is also getting more and more attention. In the past two years, major energy consuming countries have been competing to seek new energy alternatives to petroleum. Bioethanol fuel has been valued and actively supported by many countries due to its outstanding environmental protection and renewability. As a renewable resource, bioethanol can reduce the consumption of petroleum, and can be used directly as a liquid fuel or mixed with gasoline. [0003] Since 2007, large-scale outbreaks of Enteromorpha in the Yellow Sea waters of my country have occurred for 7 consecutive years. The analysis re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N1/02C12P7/06C12R1/01
CPCY02E50/10
Inventor 费岚邵飞胡乐琴何培民贾睿
Owner SHANGHAI OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com