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Fermentation strategy capable of realizing high toxoflavin production of burkholderia

A technology of Burkholderia and toxin, which is applied in the direction of fermentation, bacteria, and microorganism-based methods, can solve the problems of long experimental period and many experiments, and achieve the improvement of the ability to produce toxin, Effect of Toxoflavin Production Enhancement

Active Publication Date: 2020-05-19
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plackett-Burman Design (PBD) and Response Surface Analysis (RSM) overcome the shortcomings of the traditional single factor method, such as the large number of experiments and the long experimental cycle, and can quickly and effectively screen out the main effect factors from many influencing factors and achieve their level. optimization

Method used

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  • Fermentation strategy capable of realizing high toxoflavin production of burkholderia
  • Fermentation strategy capable of realizing high toxoflavin production of burkholderia
  • Fermentation strategy capable of realizing high toxoflavin production of burkholderia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Effect of Example 1 Inoculum Size on Burkholderia sp.HDXY-02 Toxin Production

[0056] Too large or too small inoculum is not conducive to the synthesis of the product toxin. Therefore, it is necessary to control the inoculum amount within an appropriate range during the fermentation process. Depend on figure 1 It can be seen that the content of toxic flavin in the fermentation broth increases with the increase of the inoculation amount, and when the inoculation amount is higher than 3%, the content of toxic flavin in the fermentation broth reaches the highest level, and as the inoculation amount continues to increase, the content of toxic flavin in the fermentation broth gradually increases. decline. It can be determined that the optimal inoculum size of Burkholderia sp.HDXY-02 fermentation toxiflavin is 3%, therefore, the inoculum size of 3% is selected for follow-up experiments.

Embodiment 2

[0057] Example 2 Effect of initial pH on Burkholderia sp.HDXY-02 toxin production

[0058] pH is an important factor affecting cell growth and product metabolism. The effect of different initial pH on the synthesis of toxin was studied by shaking flask culture. The result is as figure 2 shown. In the pH range of 5.0-7.0, as the pH increases, the toxin production increases gradually. When the pH is 7.0, it reaches the maximum value of 387 mg / L. When the pH is higher than 7.0, as the pH increases, Toxoflavin production begins to decline. The optimal initial pH value of Burkholderia sp.HDXY-02 fermentation toxiflavin is in the neutral range, with pH 7.0 being the best. Therefore, an initial pH of 7.0 was chosen for subsequent experiments.

Embodiment 3

[0059] Example 3 The influence of culture temperature on the production of toxin of Burkholderia sp.HDXY-02

[0060] Temperature affects the biological process of microorganisms by affecting the structure of microbial membranes, the synthesis and activity of enzymes and proteins. Depend on image 3 It can be seen from the figure that the optimal temperature for Burkholderia sp.HDXY-02 to ferment and produce toxoflavin is 30°C, which is more conducive to the synthesis of toxoflavin at 30°C. With the increase of temperature, the content of toxin flavin in Burkholderia sp.HDXY-02 fermentation broth began to decrease. Therefore, the culture temperature of 30°C was chosen for subsequent experiments.

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Abstract

The invention provides a fermentation strategy capable of improving toxoflavin production of burkholderia sp.HDXY-02, and belongs to the technical field of microbial fermentation. A strain HDXY-02 ispreserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.14054. The strategy determines that the optimal inoculation amount is 3% and the culture temperature is 30 DEG C. Through single factor experiments and statistical analysis, the optimal culture medium conditions are determined as follows: glucose (19.24 g / L), peptone (20.45 g / L), phenylalanine (0.472 g / L), K2HPO4 (1.5 g / L), MgSO4 (0.75 g / L), and pH 7.0. After optimization, the toxoflavin yield reaches 1533 mg / L and is improved by 296% than that before optimization, and the feasible fermentation strategy is provided for the high production of toxoflavin by a microbial fermentation method.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and specifically relates to a fermentation strategy for producing toxoflavin by a strain of Burkholderia, in particular a strategy for optimizing fermentation conditions for greatly increasing the yield of toxoflavin by the strain. [0002] technical background [0003] Toxoflavin is a small molecular substance synthesized by microorganisms with the molecular formula C 7 h 7 N 5 o 2 , the molar mass is 193g / mol. Studies have found that it can be used as an antibacterial and antineoplastic agent. Toxin has antagonistic ability to various pathogenic bacteria. In addition, it also has strong anti-tumor activity, and has good inhibitory activity on various cancer cells such as lung cancer cells, ovarian cancer cells, and gastric cancer cells. Therefore, as a new antibiotic and antineoplastic drug, toxin has high research value in cell apoptosis. In application, through various str...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38C12P17/18C12R1/01
CPCC12N1/20C12N1/38C12P17/182
Inventor 汪仁李晓丹陆玲周佳宇李宜奎王松风王蓉
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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