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Burkholderia and application thereof

A technology of Holderella and microbial strains, applied in the field of agricultural microorganisms, can solve the problems of large differences in soil geographical distribution, large differences in effects, and drug resistance of pathogenic bacteria, so as to reduce planting costs, high adaptability, The effect of promoting crop growth

Active Publication Date: 2020-01-10
MOON (GUANGZHOU) BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, chemical pesticides such as hymexazol and carbendazim have been used in agriculture to control Rhizoctonia solani. However, continuous long-term use of pesticides not only leads to the development of drug resistance of pathogenic bacteria, but also reduces the control effect year by year. Pesticide residues also bring environmental and social problems. Numerous negative effects
[0005]The application of existing agricultural microorganisms on crops is often relatively simple. The main function of phosphate-solubilizing bacteria is to solubilize phosphorus, but the requirements for environmental adaptability are relatively high. Due to the vast territory of our country, the composition of the soil varies greatly with the geographical distribution, which makes the same product face different soil environments, and the effect is quite different.

Method used

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  • Burkholderia and application thereof
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Embodiment Construction

[0025] Specific embodiments of the present invention will be described in detail below.

[0026] 1. Separation and identification

[0027] Burkholderia sp. MN218107 was isolated from orchids in Yonghan Town, Longmen County, Huizhou City, Guangdong Province (N23°36′42.40″, E113°56′10.45″) collected on April 9, 2018 For the rhizosphere soil, the samples were coated on organic phosphorus solid medium (glucose 10g, (NH 4 ) 2 SO 4 0.5g, yeast extract powder 0.5g, NaCl 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, Ca 3 (PO 4 ) 2 1g, MgSO 4 0.3g, 0.2g lecithin, 15-20g agar, 1L water, pH 7) to obtain by separation and screening.

[0028] The separation method is as follows: first shake the soil of the sample root system to remove the soil loosely combined with the root system, then put the root system in a 50mL triangular flask filled with 30mL sterile water, shake for about 15min, so that the soil is fully separated from the root system, and then washed The...

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PUM

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Abstract

The invention relates to a Burkholderia sp. and an application thereof. The invention relates to a Burkholderia sp., which is classified and named as Burkholderia sp., and is preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC No.16428 on September 6, 2018. The application of the Burkholderia sp. in promoting crop growth specifically comprises the step of preparing the Burkholderia sp. into a microbial seed coating agent. The Burkholderia sp. and the application thereof disclosed by the invention have the following beneficial effects: 1, theBurkholderia sp. can promote crop growth and synthesize growth hormones required by plant growth; 2, stable growth of crops can be kept in an acid-base environment within a certain range; 3, phosphorus in the soil can be converted into available phosphorus for plants, so that the planting cost is reduced, and meanwhile, the environment is protected; and 4, the growth of pathogenic bacteria can beinhibited.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a strain of Burkholderia and its application. Background technique [0002] At present, more than 70% of the cultivated land in our country is deficient in phosphorus. Although nitrogen, phosphorus, potassium and other chemical fertilizers are used on these cultivated lands, most phosphorus elements are difficult to form due to the combination of most phosphorus elements with iron ions, aluminum ions, and calcium ions in the soil. Soluble phosphate, only 10% to 20% of the phosphorus element is utilized, and the utilization rate is very low. [0003] Phosphate-solubilizing microorganism (PSM) is a special microbial group in soil, which is a kind of special microbial functional group that can convert ineffective phosphorus that is difficult for plants to absorb and utilize into available phosphorus form that can be absorbed and utilized. , which plays a key r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N51/00A01P21/00A01P3/00C05F11/08C12R1/01
CPCC12N1/20A01N63/00A01N51/00C05F11/08C12R2001/01C12N1/205Y02A40/10
Inventor 邹秋霞余昕彤
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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