Burkholderia and application thereof
A technology of Holderella and microbial strains, applied in the field of agricultural microorganisms, can solve the problems of large differences in soil geographical distribution, large differences in effects, and drug resistance of pathogenic bacteria, so as to reduce planting costs, high adaptability, The effect of promoting crop growth
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[0025] Specific embodiments of the present invention will be described in detail below.
[0026] 1. Separation and identification
[0027] Burkholderia sp. MN218107 was isolated from orchids in Yonghan Town, Longmen County, Huizhou City, Guangdong Province (N23°36′42.40″, E113°56′10.45″) collected on April 9, 2018 For the rhizosphere soil, the samples were coated on organic phosphorus solid medium (glucose 10g, (NH 4 ) 2 SO 4 0.5g, yeast extract powder 0.5g, NaCl 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, Ca 3 (PO 4 ) 2 1g, MgSO 4 0.3g, 0.2g lecithin, 15-20g agar, 1L water, pH 7) to obtain by separation and screening.
[0028] The separation method is as follows: first shake the soil of the sample root system to remove the soil loosely combined with the root system, then put the root system in a 50mL triangular flask filled with 30mL sterile water, shake for about 15min, so that the soil is fully separated from the root system, and then washed The...
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