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Bacterial strain resistant to acid-aluminum

An acid-resistant aluminum and bacterial strain technology, applied in the field of microorganisms, can solve the problems of difficulty, uneconomical, a lot of manpower, material resources and financial resources, and achieve the effect of strong adsorption, strong acid-resistant aluminum performance, and good microbial materials.

Inactive Publication Date: 2014-09-03
JINGGANGSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the application of lime and fertilizer can alleviate or prevent the crop growth problems caused by the acid aluminum in the surface soil, it is difficult and economical to apply lime or fertilizer to the deep substratum soil, and these measures need to invest a lot of manpower, material and financial resources. And it cannot be done once and for all, it is difficult to obtain good economic and ecological benefits

Method used

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  • Bacterial strain resistant to acid-aluminum
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  • Bacterial strain resistant to acid-aluminum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The isolation of embodiment one bacterial strain SB1:

[0023] (1) Fresh red loam samples were collected in Ji'an City, Jiangxi Province. The basic physical and chemical properties of the soil samples were pH: 4.46, containing OM / : 17.8g / kg -1 , AN / : 127.5mg / kg -1 , AP / : 4.05mg / kg -1 , AK / : 48.5mg / kg -1 , EC / 0.15dS / m -1 , Monomeric Al: 42.6 μmol / L. Take 5g of fresh soil in the sample and add 10mL of sterile water to make a soil solution; take 1mL of soil solution in 99mL of sterilized GM liquid medium. Then add 50 μL 1mol / L sterile AlCl to the medium 3 ·6H 2 O solution, placed in a shaking incubator at 37°C with a vibration frequency of 260rpm / min for 15h.

[0024] The above GM medium is: glucose 10g, peptone 0.5g, yeast extract 0.2g, magnesium sulfate (MgSO 4 ·7H 2 O) 0.2g, dilute to 1000mL, in the solution bottle of pH5.0.

[0025] (2) Take 1 mL of the bacterial solution obtained in step (1), add it to a 250 mL Erlenmeyer flask containing sterilized 99 ml li...

Embodiment 2

[0030] The cultivation and molecular identification of embodiment two strain SB1:

[0031](1) The 16S rDNAs of 8 strains were amplified by nested-PCR. Methods as below:

[0032] The first round reaction (volume 25 μL) contains 1 μL (about 1 ng) DNA as template, 0.5 μL primer (10 μM), 2 μL dNTP mixture (2.5 mM) reagent, 1.5 μL MgCl 2 (25 mM), 2.5 μL of 10×PCR reaction buffer and 0.2 μL of Taq DNA polymerase (5 U / μL, Takara Company). The primers for the first round of reaction were: 27f (5'-AGAGT TTGAT CCTGG CTCAG-3') and 1492r (5'-TACCT TGTTA CGAC TT-3'). The PCR reaction program was: 94°C pre-denaturation for 5 min, 94°C for 30 s, 55°C for 40 s, 72°C for 1 min, a total of 10 cycles, and finally 72°C for 10 min. The second round reaction uses the PCR product of the first round reaction as a template, and the primers are: 63f (5'-CAGGC CTAAC ACATG CAAGT C-3') and 1389r (5'-ACGGG CGGTG TGTAC AAG-3'). In this round of reaction, 30 cycles were set, and the reaction conditions ...

Embodiment 3

[0036] Example 3 Growth experiments of bacterial strain SB1 under different aluminum stress concentrations:

[0037] Take 30 μL of the bacterial solution stored in the refrigerator at -80°C and inoculate them with aluminum treatment concentrations: 0, 1, 2, 3, 4mmol / L AlCl 3 ·6H 2 Put 3mL of fresh GM medium into a test tube of O, and place the test tube in a shaking incubator with a temperature of 37°C and a vibration frequency of 260r / min for 15h. And each aluminum treatment concentration is set 12 groups of repetitions, each group of test tube bacteria liquid is sampled respectively every 2 hours (with the culture medium that does not inoculate bacteria as contrast), measures its OD600 value, draws the growth curve of SB1 bacterial strain. The results showed that the addition of aluminum at the initial stage of bacterial growth would affect the growth of bacteria, and the higher the concentration, the longer the time for bacteria to enter the exponential growth phase. At t...

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Abstract

The invention discloses a bacterial strain resistant to acid-aluminum. The bacterial strain belongs to beta-proteobacteria burkholderia sp., is named as Burkholderia sp.SB1, and is collected in China General Microbiological Culture Collection Center (CGMCC) in September, 2013, with the collection number of CGMCC No. 8396. The bacterial strain has beneficial effects that the bacterial strain shows stronger acid-aluminum resistance performance, and has stronger adsorption effect on aluminum, so that a good microbial material is further provided for improving soil contaminated by aluminum by utilizing the bacterial strain.

Description

Technical field: [0001] The invention relates to bacteria in the field of microorganisms, in particular to an aluminum-resistant bacterial strain. Background technique: [0002] Aluminum is a non-essential element for the normal growth of organisms. In slightly acidic and neutral soils, aluminum mainly exists in trivalent oxidation states and stable mineral forms, showing extremely low bioavailability. However, as the pH of the soil decreases, aluminum can gradually dissociate and be released into the soil solution in an ionic state, becoming an active monomeric form (monomeric Al), which has a very toxic effect on soil organisms. In recent years, with the extensive use of acidic precipitation and chemical fertilizers, the concentration of active aluminum in acidic red soil has been increasing, which has seriously affected the growth of crops. This pollution situation has aroused widespread concern in the academic circles. [0003] How to reduce the toxicity of aluminum in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/22
Inventor 贺根和林俊岳吴吉春张友海
Owner JINGGANGSHAN UNIVERSITY
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