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Preparation method and application of burkholderia sp. MEL01 capable of efficiently antagonizing fusarium graminearum

A technology of Fusarium graminearum and the genus of fungus, applied in the field of microorganisms, can solve the problems of insufficient fermentation methods, achieve the effects of reducing the disease index, good application prospects, and increasing the inhibition rate

Active Publication Date: 2020-09-01
随州市楚天园艺有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant research report on improving the activity of Burkholderia against Fusarium graminearum and improving the antibacterial activity of Burkholderia

Method used

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  • Preparation method and application of burkholderia sp. MEL01 capable of efficiently antagonizing fusarium graminearum
  • Preparation method and application of burkholderia sp. MEL01 capable of efficiently antagonizing fusarium graminearum
  • Preparation method and application of burkholderia sp. MEL01 capable of efficiently antagonizing fusarium graminearum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1MEL01 is to the plate confrontation activity of fungus such as Fusarium graminearum

[0036] Inoculate the fungus on the center of the PDA medium plate. After the fungus is activated, use a hole puncher with a diameter of 7mm to punch the fungus cake at the edge of the colony, inoculate the fungus cake on one side of the center of the plate, and at the same time, line MEL01 symmetrically on the center of the plate On the other side, the distance between the fungal cake and the antagonist MEL01 is 40mm. After the control strain was overgrown, the width of the inhibition zone (the distance between the edge of the antagonistic bacteria colony and the edge of the Fusarium graminearum colony) was taken as a control without inoculating the antagonistic antibacterial strain Burkholderia sp.MEL01. Each treatment was repeated 3 times.

[0037]Bacterial inhibition rate (%)=(control colony diameter-treated colony diameter) / control colony diameter×100%.

[0038] The p...

Embodiment 2

[0039] The influence of embodiment 2 different bacterium concentrations MEL01 on the spore germination of Fusarium graminearum

[0040] The bacterial concentration was 5.0×10 1 ~5.0×10 8 CFU / mL of MEL01 bacterial suspension and 2.0×10 5 CFU / mL Fusarium graminearum spores were mixed thoroughly, co-cultivated at 25°C for 6 hours, transferred to a PDA plate and cultured for 2 days to observe whether the spores of Fusarium graminearum germinated to form hyphae. Such as figure 2 As shown, 5.0×10 5 The MEL01 bacterial suspension at the concentration of CFU / mL bacteria can completely inhibit the germination of Fusarium graminearum spores to form hyphae.

Embodiment 3

[0041] Example 3 Effects of Different Culture Modes on the MEL01 Fermentation Supernatant Inhibiting the Activity of Fusarium graminearum

[0042] Pick a single colony of Burkholderia sp.MEL01 and insert it into sterilized LB medium for overnight activation. The activated MEL01 was washed twice with sterile water at 8000 rpm for 10 min, and then inserted into 500 mL of sterile PDB fermentation medium at an inoculum size of 5% (5% is the volume percentage of the fermentation medium).

[0043] Group A was cultured in shake flasks at 28°C and 180 rpm for 5 days;

[0044] Group B was shaken at 180 rpm at 28°C for 2 days, then cultured at 28°C for 3 days;

[0045] Group C was cultured in shake flasks at 28°C and 180 rpm for 2 days, then added appropriate amount of mycelium of Fusarium graminearum every other day for induction, adding one 90mm plate mycelium each time.

[0046] Centrifuge the three groups of fermentation broth at 12,000rpm for 15min, concentrate it by rotary evapo...

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Abstract

The invention relates to a preparation method and application of burkholderia MEL01 capable of efficiently antagonizing fusarium graminearum, and belongs to the technical field of microorganisms. Themethod comprises the following steps: carrying out shake-flask culture on Burkholderia sp. MEL01 for 2 days and then carrying out static culture for 3 days, or continuously carrying out shake-flask culture for 3 days after carrying out shake-flask culture for 2 days; adding fusarium graminearum hyphae every day continuously for three times. The inhibition activity of the supernatant obtained by the fermentation method on fusarium graminearum hyphae is obviously improved; when the method is used in the early flowering stage of wheat, the disease index and DON synthesis can be remarkably reduced; and the method is simple and easy to operate, and the antibacterial activity of the Burkholderia sp. MEL01 (Burkholderia sp.) fermentation supernatant can be remarkably improved.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a preparation method and application of a strain of Burkholderia genus MEL01 that can efficiently antagonize Fusarium graminearum. Background technique [0002] Fusarium graminearum (Fusarium graminearum) is one of the important pathogens that can cause head blight in many grass crops such as wheat, barley, rice and corn. It will cause the ear of the crop to be diseased, and the grain will shrink, thereby reducing the crop yield. More importantly, in the process of Fusarium graminearum infecting crops, it will produce a toxic metabolite, deoxynivalanol (DON), which is stable in nature, resistant to heat, pressure, weak acid, Storage resistance, the general food processing process cannot destroy its structure, only adding alkali or high pressure treatment can destroy some toxins. When people ingest food made from DON-contaminated grains, it may cause vomiting, diarrhea, headach...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCC12N1/20A01N63/20C12R2001/01C12N1/205
Inventor 倪红王行国李亚东陈玲孙孝文许银姚伦广王友平杨立军陈勋
Owner 随州市楚天园艺有限公司
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