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Efficient phosphate-dissolving bacterium and produced bacterial agent thereof

A technology of phosphorus-solubilizing bacteria and bacterial agents, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of low phosphorus conversion rate, low utilization rate of chemical phosphate fertilizer, and easy pollution of the environment, so as to improve soil environment and fertility, The effect of increasing crop yield and reducing environmental pollution

Active Publication Date: 2014-07-09
河南金百合生态农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to develop a high-efficiency microbial phosphorus-dissolving bacteria agent for the practical problems of low conversion rate of phosphorus in crop soil, low utilization rate of chemical phosphorus fertilizer and easy pollution of the environment, as well as the environmental protection and effective characteristics of biological phosphorus fertilizer

Method used

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  • Efficient phosphate-dissolving bacterium and produced bacterial agent thereof
  • Efficient phosphate-dissolving bacterium and produced bacterial agent thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the production of phosphate solubilizing bacteria agent

[0018] (1) Shake flask culture

[0019] Activate phosphate-solubilizing bacteria 21-III on a beef extract peptone culture dish, streak and pick a single colony for two cultures, then pick a single colony and inoculate it on the slant medium of the test tube for later use; the strain on the slant medium of the test tube , inoculated in a triangular flask containing beef extract peptone medium, and cultured in a shake flask until the logarithmic growth phase. The culture conditions were 26-32 ℃, rotation speed 140-200 rpm, liquid volume in 500 mL Erlenmeyer flask was 150-300 mL, and culture time was 24-36 h. The composition of beef extract-peptone medium is beef extract 3 g / L, peptone 10 g / L, NaCl 5 g / L, pH 7.0-7.2.

[0020] (2) Fermentation tank culture

[0021] Inoculate the above-mentioned shake flask bacterial liquid in the fermentation medium of phosphate-solubilizing bacteria agent, the inocu...

Embodiment 2

[0022] Embodiment 2: laboratory calcium phosphate dissolution experiment

[0023] After the activation of strain 21-III on the beef extract peptone medium plate, inorganic calcium phosphate was used as an insoluble phosphorus source to prepare inorganic phosphorus medium and inoculate 21-III with an inoculation ratio of 5%. At the same time, a parallel system without bacteria was set up as a control. In order to eliminate the interference of the system itself. The culture solution was regularly and quantitatively sampled, filtered through a 0.22 μm microporous membrane, and the molybdenum antimony anti-spectrophotometric method was used to measure the content of soluble phosphate. After excluding the control value, the results were shown in figure 1 As shown, it shows that at 72 h, the amount of phosphorus dissolved is the largest, reaching 16.6 μmol / mL (calculated as PO4), which can effectively dissolve insoluble particulate calcium phosphate.

[0024] In this embodiment 2,...

Embodiment 3

[0025] Example 3: Soil Phosphorus Solubilization Efficiency

[0026] The test soil was collected from farmland in the suburbs of Zhengzhou City, and the sampling depth was 0-20 cm. Remove impurities after collection. Add 90 mL of sterile water to 30 g of soil. Inoculate 10 mL of 21-III bacterial agent as the treatment group; at the same time, inoculate 10 mL of inactivated 21-III bacterial agent as the parallel control group. Under normal temperature conditions, let it stand still, take the supernatant at a predetermined time point, and measure the soluble available phosphorus content therein. see results figure 2 shown. The results showed that the dissolution of soil phosphorus by the bacterial agent reached the highest at 48 h, and compared with the control group, the content of soluble phosphorus in the soil increased by 1.87 times under the action of the bacterial agent.

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Abstract

The invention provides an autonomously-screened efficient phosphate-dissolving bacterium and a produced bacterial agent thereof, and belongs to the technical field of biology. The bacterial strain of the phosphate-dissolving bacterium is named as 21-III, is identified as burkholderia sp., and is preserved in China General Microbiology Center of Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No. 7873. 16SrDNA sequence of the bacterial strain is submitted to GenBank, and the registration number is JX857813. The main biological characteristics of the bacterial strain comprise that Gram staining is negative, thallus is in the shape of a rod, catalase reaction is positive, oxidase reaction is positive and methyl red reaction is negative. The bacterial agent has obvious promotion effect on dissolution rate of phosphorus in soil, and is applicable to improve soil fertility of crops and beneficial for environment protection.

Description

technical field [0001] The invention relates to a high-efficiency phosphorus-dissolving bacterium and a bacterial agent produced therefrom, which belong to the field of biotechnology and use microorganisms to improve the dissolution efficiency of soil inorganic phosphorus, which is suitable for improving the phosphorus-dissolving efficiency of farmland soil and serving the growth of crops. Background technique [0002] In agricultural production, phosphorus is one of the indispensable important nutrients for crop growth and development, but because most of the phosphorus in the soil is in the state of organic or inorganic phosphide, plants cannot directly absorb and utilize it. More than 70% of the cultivated land in my country lacks available phosphorus, so phosphorus is one of the important factors that limit plant growth and increase crop production. At present, water-soluble chemical fertilizers are mostly used in production, but after the application of phosphorus ferti...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/00C12R1/01C09K101/00
Inventor 屈建航屈建莹郭娟李海峰周佳何晓冰翟焕趁
Owner 河南金百合生态农业科技有限公司
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