Attapulgite (ATT)-doped PLGA nano-fiber felt, as well as preparation method and application thereof

A technology of attapulgite and PLGA, which is applied to PLGA nanofiber mat and its preparation and application fields, and achieves the effects of low raw material cost, easy product availability and good biocompatibility

Inactive Publication Date: 2015-01-07
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no literature report on the preparation of PLGA / ATT composite nanofibers by electrospinning, and further evaluation of the mechanical properties, biological activity and hemocompatibility of the composite nanofibers and their promotion of mesenchymal stem cell differentiation.

Method used

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  • Attapulgite (ATT)-doped PLGA nano-fiber felt, as well as preparation method and application thereof
  • Attapulgite (ATT)-doped PLGA nano-fiber felt, as well as preparation method and application thereof
  • Attapulgite (ATT)-doped PLGA nano-fiber felt, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Take 4 parts of 0.8g PLGA, mix them with 2.4mL THF and 0.8mL DMF respectively, and stir at room temperature for 8h until PLGA is completely dissolved. 8mg, 16mg and 24mg of ATT powder were added to the 2nd, 3rd and 4th PLGA solution, respectively. Magnetic stirring was used for 20 min at a stirring rate of 200 r / min to obtain PLGA and PLGA / ATT spinning solutions. PLGA, PLGA / 1%ATT, PLGA / 2%ATT and PLGA / 3%ATT nanofiber mats were prepared by electrospinning. Among them, the receiving distance is 15cm, the voltage is 20kV, the flow rate is 0.8mL / h, and the prepared composite nanofiber mat is dried in a vacuum oven for 48 hours to remove residual moisture and solvent.

[0070] SEM observation results show that the obtained PLGA, PLGA / 1% ATT, PLGA / 2% ATT and PLGA / 3% ATT nanofibers have regular morphology and regular surface, and the fiber diameters are 1308±296nm, 909±185nm, 560± 117nm and 483±133nm (see attached figure 1 ). Comparing the diameters of four different fibers...

Embodiment 2

[0072] The PLGA, PLGA / 1%ATT, PLGA / 2%ATT and PLGA / 3%ATT nanofiber mats prepared in Example 1 of a certain quality (about 5mg) were weighed respectively and carried out TGA test. figure 2 Shown are TGA curves for PLGA, PLGA / 1% ATT, PLGA / 2% ATT and PLGA / 3% ATT nanofiber mats. From figure 2 It can be seen that it is obvious that when the treatment temperature reaches 600 °C, the organic component (PLGA) in the nanofibers has been completely burned (residual mass is 0); while ATT has basically not changed (residual mass is 98.7wt.%) . The residual mass of PLGA / 1wt.% LAP, PLGA / 2wt.% LAP and PLGA / 3wt.% LAP composite nanofiber is respectively 0.98wt.%, 1.87wt.% and 3.17wt.%. ATT approximation added in .

Embodiment 3

[0074] The PLGA, PLGA / 1%ATT, PLGA / 2%ATT and PLGA / 3%ATT nanofiber mats prepared in Example 1 were cut into strips of 1cm×5cm, and 3 parallel samples were taken for each sample. Measure the thickness of 3 different positions of each fiber mat with a micrometer, and calculate the average value as the thickness of each sample. Test the mechanical properties of the fiber felt with a universal material testing machine, and draw the stress-strain curve (see attached image 3 ). It can be seen from the stress-strain curve that after a certain amount of ATT is doped in PLGA fibers, the fracture strength of PLGA nanofibers can be significantly improved.

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Abstract

The invention relates to an attapulgite (ATT)-doped PLGA nano-fiber felt, as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving PLGA in a mixed solvent to obtain a PLGA electrospinning solution; uniformly dispersing ATT in the PLGA electrospinning solution while stirring to obtain a PLGA/ATT electrospinning solution; and electrospinning by adopting the PLGA/ATT electrospinning solution for preparation. Nano-fibers promote human mesenchymal stem cells (hMSC) to be differentiated into bone cells. According to the preparation method provided by the invention, the process is simple, the product is easy to obtain, and the cost of used PLGA and ATT is relatively low; the prepared ATT-doped PLGA nano-fibers have good mechanical properties, blood compatibility and biocompatibility, and have broad application prospects in the fields of pharmaceutical carriers, tissue engineering scaffold materials and the like.

Description

technical field [0001] The invention belongs to the field of nanofiber felt material and its preparation and application, in particular to a PLGA nanofiber felt doped with attapulgite and its preparation and application. Background technique [0002] As a new processing method for nanomaterials, electrospinning technology has the advantages of adjustable and controllable processing parameters, high utilization rate of raw materials, diversified fiber collection methods, high experimental repeatability and easy industrialization. The prepared electrospinning polymer Nanofibers have the characteristics of controllable fiber size, large specific surface area, high porosity and three-dimensional network structure, and can well simulate natural extracellular matrix (ECM), making electrospinning technology and its products widely used in many fields. It has been widely used in the field. Especially in the field of biomedical engineering, natural polymers and synthetic polymers ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/38A61K47/34A61K47/04
Inventor 史向阳王哲赵毅丽
Owner DONGHUA UNIV
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