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Icariin controlled release chitosan/hydroxyapatite composite scaffold material and preparation method thereof

A technology of hydroxyapatite and icariin, applied in the field of biomedical materials, can solve problems such as limited loading, and achieve the effects of improving bone repair performance, prolonging release time, and easy raw materials

Active Publication Date: 2015-02-25
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current loading form of Ica is also a non-covalent loading, such as "A new bone repair scaffold combined with chitosan / hydroxyapatite and sustained releasing icarrin" published by Wu Tao et al. (see Chinese Science Bulletin.2009; 17: p2953 -2961) introduced Ica into the CHS / HA composite scaffold material in a non-covalent manner, which inevitably caused the initial burst phenomenon
On the other hand, since when the concentration of icariin is greater than 1×10 -5 M has obvious cytotoxicity, although the solubility of icariin is low, the concentration of the saturated solution obtained at room temperature also reaches 2×10 -5 M, has a significant inhibitory effect on the growth of cells, coupled with the sudden release of non-covalent loading, resulting in a very limited loading of icariin directly in a non-covalent form

Method used

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  • Icariin controlled release chitosan/hydroxyapatite composite scaffold material and preparation method thereof
  • Icariin controlled release chitosan/hydroxyapatite composite scaffold material and preparation method thereof
  • Icariin controlled release chitosan/hydroxyapatite composite scaffold material and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0054] (1) Preparation of raw material solution

[0055] Using chitosan, hydroxyapatite and succinylated icariin as raw materials, the mass ratio of chitosan to hydroxyapatite is 1:9, and the raw material solution is prepared according to the following method:

[0056] Under normal pressure and room temperature, use 0.5% dilute hydrochloric acid as solvent to prepare chitosan solution, and the consumption of hydrochloric acid reaches 30mg / ml as the limit with the concentration of chitosan in chitosan solution;

[0057] Hydroxyapatite was prepared into a 27% w / v slurry with deionized water at normal pressure and room temperature;

[0058] Under normal pressure and room temperature, the succinylated icariin was formulated with 0.2mol / L sodium hydroxide to make 1×10 -3 mol / L solution;

[0059] The structural formula of the succinylated icariin is:

[0060]

[0061] (2) Preparation of icariin controlled-release chitosan / hydroxyapatite composite scaffold material

[0062] St...

Embodiment 2

[0065] (1) Preparation of raw material solution

[0066] Using chitosan, hydroxyapatite and succinylated icariin as raw materials, the mass ratio of chitosan to hydroxyapatite is 1:1, and the raw material solution is prepared according to the following method:

[0067] Under normal pressure and room temperature, the hydrochloric acid with the volume concentration of 0.7% is the solvent preparation chitosan solution, and the consumption of hydrochloric acid reaches 60mg / mL as the limit with the concentration of chitosan in the chitosan solution;

[0068] Hydroxyapatite is prepared into a 12% w / v slurry with deionized water or distilled water at normal pressure and room temperature;

[0069] At normal pressure and room temperature, the succinylated icariin was formulated with a 0.2mol / L sodium hydroxide solution to a concentration of 1×10 -1 mol / L solution;

[0070] (2) Preparation of icariin controlled-release chitosan / hydroxyapatite composite scaffold material

[0071] The ...

Embodiment 3

[0074] (1) Preparation of raw material solution

[0075] With chitosan, hydroxyapatite and succinylated icariin as raw materials, the mass ratio of chitosan to hydroxyapatite is 2:3, and the raw material solution is prepared according to the following method:

[0076] Under normal pressure and room temperature, the hydrochloric acid with the volume concentration of 1% is the solvent preparation chitosan solution, and the consumption of hydrochloric acid reaches 40mg / mL as the limit with the concentration of chitosan in the chitosan solution;

[0077] Hydroxyapatite was prepared into a 6% w / v slurry with deionized water at normal pressure and room temperature;

[0078] At normal pressure and room temperature, the succinylated icariin was formulated with a 0.5mol / L sodium hydroxide solution to a concentration of 1×10 -1 mol / L solution;

[0079] (2) Preparation of icariin controlled-release chitosan / hydroxyapatite composite scaffold material

[0080] Stir and mix the chitosan ...

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Abstract

The invention discloses a preparation method of an icariin controlled release chitosan / hydroxyapatite composite scaffold material. The method comprises the following steps: (1) preparing a raw material solution; and (2) stirring and mixing a chitosan solution with hydroxyapatite slurry evenly to obtain mixed slurry, dropwise adding a succinic acid icariin solution at the amount of keeping the final concentration of succinic acid icariin in the mixed slurry at 1*10<-5> to 1*10<-2> mol / L, adding a cross-linking agent, adjusting the pH to be 5.0-6.5, further stirring and reacting for 10-120 minutes, centrifuging reaction liquid and removing bubbles after reaction is ended, injecting into a mold, and standing for 10-240 minutes to obtain a gel product; freezing, drying, washing, and freezing and drying again. The composite scaffold material disclosed by the invention is capable of improving the bone repair performance of the scaffold material through proliferation and differentiation of slowly released Ica regulating cells.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a composite support material for bone tissue defect repair and a preparation method thereof. Background technique [0002] Bone defect is a common clinical disease. Bone tissue engineering is currently the most promising technology for treating bone defects. Artificial bone tissue is composed of scaffolds and seed cells prepared from biomaterials. In order to better promote cell proliferation and osteogenic differentiation, and improve the efficiency of bone defect repair, many exogenous osteoinductive growth factors (such as bone morphogenic protein BMP) and exogenous growth factors (such as small plant molecules that promote osteogenesis ) was introduced into the scaffold material to form a composite scaffold material. However, since the current growth factors are loaded into the scaffold material in a non-covalent manner (such as physical packaging, physical adsorption and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/42A61L27/54A61L27/56
Inventor 肖玉梅杨继榕蔡希何龙袁暾李东晓张兴栋
Owner SICHUAN UNIV
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