The invention provides an exosome loaded icariin nano preparation, which is prepared by loading icariin on cow's milk exosomes, and the preparation method comprises the following steps: performing centrifuging for 30-60 minutes by using a centrifugal machine at 4 DEG C and 13,000-15,000 g, taking middle layer whey, and performing filtering by using a vacuum pump with 600-800 meshes; performing centrifuging at the temperature of 4 DEG C at 100000-150000 g for 1-2 hours, and taking the whey in the middle layer; and performing filtering by using a 1000-2000 mesh vacuum pump, performing centrifuging for 1-2 hours at the temperature of 4 DEG C under the condition of 13000-15000g, reserving 1-2 ml of whey at the bottom, collecting the whey in a 50ml EP tube, performing centrifuging for 1-2 hours at the temperature of 4 DEG C under the condition of 100000-150000g, discarding the supernatant, and separating out the milk exosome; mixing icariin and the exosome suspension at room temperature according to a ratio of 1: 9; and performing freezing and drying to obtain the exosome loaded icariin nano-drug. The material has the unique advantages that the availability is high, the cost is low, the toxicity is low, the yield is high, the medicine can be orally delivered, the large-scale production can be achieved, the bioavailability of the oral medicine can be potentially enhanced, the efficacy and the safety of the medicine can be improved, and the bone repair capability of the hostoside can be improved.