35results about How to "Improve bone repair ability" patented technology

The invention belongs to the field of bio-medicinal material science, and particularly relates to a method for preparing an aqueous gel / nano hydroxyapatite composite scaffold for hard tissue repair. The method comprises the following steps of: preparing a small blood vessel by an electrostatic spinning method; preparing a degradable three-dimensional scaffold material taking the small blood vessel as a reserved passage inner wall by using a hot pressing / salting-out method; injecting injectable super-molecular aqueous gel prepared from cyclodextrin, tri-block polymer and nano hydroxyapatite into the reserved passage; and soaking the obtained scaffold into growth factor culture liquid for modification. The composition, property and shape of the prepared scaffold material are more suitable for bone surface blood vessel formation, and new cells and nutrients can enter the inner side of the scaffold through the aqueous gel in the reserved passage to induce bone growth so as to better finish the repair of bone defects. The bone repair effect of the scaffold material is obviously improved compared with other bone repair materials, and the mechanical property, blood vessel function and bone repair function of the scaffold material are obvious compared with common bone repair materials.

The invention provides functionalized mesoporous bioglass porous scaffolds as well as a preparation method and an application thereof. The preparation method comprises the following steps: (1) providing mesoporous bioglass powder; (2) uniformly mixing the mesoporous bioglass powder, a binding agent and a pore-foaming agent in a mass ratio of (0.3-0.5): (0.05-0.15): (0.3-0.5) so as to obtain mixed powder, putting the mixed powder into a mold, and pressing at the pressure of 2-5 Mpa; (3) putting and baking materials obtained by pressing in a muffle furnace so as to obtain porous scaffolds; (4) respectively putting the scaffolds into a methylbenzene solution containing a silane coupling agent APS (aminopropyltriethoxysilane) and a methylbenzene solution containing 3-(triethoxysilyl)propylsuccinic anhydride (TESPSA), refluxing, and drying in a vacuum so as to respectively obtain the amination mesoporous bioglass porous scaffold and the carboxylation mesoporous bioglass porous scaffold. The porous scaffolds provided by the invention have the advantages that the mesoporous structure of mesoporous bioglass is maintained, and the porous scaffolds are relatively high in porosity and large in capacity, have relatively high strength and can be used for bone tissue repairing.

The invention discloses a nano-composite material used for tissue repair, which comprises segmented copolymer with electrical activity, nano-hydroxyapatite and normal tissue repair materials; wherein the segmented copolymer comprises aliphatic polyester and ailine pentamer, and the normal tissue repair materials comprises poly (lactide-co-glycolide), polylactide or polycaprolactone. The invention also discloses a preparation method of the nano-composite materials used for tissue repair, which comprises the following steps of: (a) dissolving the normal tissue repair material in a solvent, then adding the nano-hydroxyapatite into the solvent, and uniformly stirring to obtain a substrate material; and (b) dissolving the segmented copolymer into the solvent, then adding to the substrate material and mixing uniformly, and then precipitating and removing the solvent. The nano-composite material used for the tissue repair has the advantages of nontoxicity, biodegradability, processibility, sufficient mechanical strength, favorable electrical activity, and biocompatibility.

The invention discloses a preparation method of an icariin controlled release chitosan / hydroxyapatite composite scaffold material. The method comprises the following steps: (1) preparing a raw material solution; and (2) stirring and mixing a chitosan solution with hydroxyapatite slurry evenly to obtain mixed slurry, dropwise adding a succinic acid icariin solution at the amount of keeping the final concentration of succinic acid icariin in the mixed slurry at 1*10<-5> to 1*10<-2> mol / L, adding a cross-linking agent, adjusting the pH to be 5.0-6.5, further stirring and reacting for 10-120 minutes, centrifuging reaction liquid and removing bubbles after reaction is ended, injecting into a mold, and standing for 10-240 minutes to obtain a gel product; freezing, drying, washing, and freezing and drying again. The composite scaffold material disclosed by the invention is capable of improving the bone repair performance of the scaffold material through proliferation and differentiation of slowly released Ica regulating cells.

The invention discloses a polyether-ether-ketone bone repair material with multi-scale holes and a preparation method thereof, and belongs to the technical field of medical biological materials. The preparation method comprises the following steps: firstly, hot-pressing polyether-ether-ketone granules into a plate, after cleaning and drying, obtaining a partially foamed polyether-ether-ketone plate in a supercritical carbon dioxide foaming device, conducting sulfonation in a mixed acid solution of concentrated sulfuric acid and methane sulfonic acid, and conducting hydrothermal treatment and vacuum drying to obtain the polyether-ether-ketone bone repair material with multi-scale holes. Small-size micro-nano holes obtained through sulfonation are beneficial to adhesion of early osteoblasts, large-size micro holes obtained through supercritical carbon dioxide foaming are beneficial to ingrowth of bone tissues in a later period, the material has a multi-scale hole structure through simple two-step operation, and bone repair is promoted. Compared with a hole-making technology using a hole-making agent, a supercritical carbon dioxide foaming technology does not have a problem that the hole-making agent is not thoroughly removed and the possibility that the material is applied to the field of biomedicine is ensured.

The invention belongs to the field of biomedical composite materials and discloses a modified nano-hydroxyapatite supramolecular composite hydrogel and a preparation method thereof. The preparation method comprises the following steps: modifying natural molecule dopamine to the surface of nanometer hydroxyapatite through in-situ polymerization, grafting polyethylene glycol to the dopamine-coated nanometer hydroxyapatite through Schiff base reaction, simply blending a polyethylene glycol-grafted hydroxyapatite turbid liquid and an alpha-cyclodextrin aqueous solution for self-assembly to obtainthe modified nano-hydroxyapatite supramolecular composite hydrogel for bone repair. The preparation method of the composite hydrogel has the advantages that a synthetic route is simple, and the gelation process is easy to operate. Through regulating the proportion of the nanometer hydroxyapatite, the mechanical properties of the hydrogel can also be regulated, so that the hydrogel can adapt to a wider range of biomedical applications.

The invention discloses a high-strength mineralized hydrogel and applications of the high-strength mineralized hydrogel in bone repair. According to the present invention, N-acryloyl glycinamide and vinylphosphonic acid are used as raw materials and are co-polymerized in the presence of an initiator to prepare a hydrogel, calcium ions and phosphorus ions are deposited in situ in the gel to form hydroxyapatite, the mineralized hydrogel pipe has good tensile performance and good compressive performance due to the physical cross-linking effect of the hydrogen bond and the ion cross-linking effectof the hydroxyapatite and the gel matrix in the gel structure, and due to the presence of hydroxyapatite, the osseointegration and bone repair ability of the gel pipe is improved.

The invention provides a preparation method of hydrogel, osteoblast containing hydrogel and a preparation method of the osteoblast containing hydrogel.The preparation method of the hydrogel includes the following steps that S1, dialdehyde hyaluronic acid and RGD are provided; S2, the objective ratio needed by dialdehyde hyaluronic acid and RGD is obtained through the high-throughput screening technology; S3, dialdehyde hyaluronic acid and RGD in S2 are crosslinked to form the needed hydrogel with a three-dimensional porous structure.The concentrations needed by the dialdehyde hyaluronic acid water solution and the RGD water solution and the objective ratio for mixing of the dialdehyde hyaluronic acid water solution and the RGD water solution are rapidly obtained through the high-throughput screening technology, and therefore the hydrogel material with the needed hole diameter size and porosity can be obtained, the hydrogel is beneficial for cell growth, especially, osteoblast can obtain better growth, differentiation and propagation conditions in the hydrogel material, the cell survival rate is effectively increased accordingly, and the bone repair effect is effectively improved accordingly.

The invention discloses PLGA / hydroxyapatite / calcium carbonate compound microspheres and a preparation method of the PLGA / hydroxyapatite / calcium carbonate compound microspheres. The preparation method of the compound microspheres comprises the following steps of: (1) adding hydroxyapatite and calcium carbonate into a PLGA inorganic solution to obtain a mixture solution; (2) dissolving glucolactone into a PVA (polyvinyl alcohol) aqueous solution to obtain an acidic PVA aqueous solution; (3) adding the mixture solution obtained in step (1) into the acidic PVA aqueous solution, and forming single emulsion of oil in water; and (4) mixing the single emulsion to volatize dichloromethane, curing emulsion drops to be spheres, collecting the obtained microspheres, washing by de-ionized water, and freezing and drying to obtain the compound microspheres. The microspheres obtained by the method disclosed by the invention are regular in appearance, have controllable and porous surfaces, and do not need any externally-added pore-forming agent. The compound microspheres are better in medicine sustained-release capacity and are beneficial to the growth of the cells on the surfaces.

The invention discloses artificial bones for recovering bone defects of osteoma. The artificial bones are prepared from polylactic acid powder (PLA), hydroxyapatite powder (HA) and dimethylbiguanide powder (MET) according to mass ratios of (8 to 10) to 1 to (3 to 5). According to the artificial bones disclosed by the invention, by using dimethylbiguanide as a main effect increasing component, the aims of suppressing tumor cells and enhancing bone recovering ability are fulfilled, and the defect that the bone defects of the osteoma easily and locally recur, in particular to benign or borderline tumors such as giant cell tumors of bones is overcome; and meanwhile, because of excellent bone recovery performance, the challenge that self-recovery of massive bone defects is difficult after tumor excision is performed can be solved.

The invention relates to a preparation method of a multistage micro-nano structure bone repair scaffold for freeze-drying delivery of exosomes. The method comprises the following steps: a, carrying out cell culture on supernatant to obtain an exosome concentrated solution; and b, adsorbing the exosome concentrated solution to an MBG stent with a macroporous / microporous / mesoporous multilevel structure, and performing freeze drying. The multistage micro-nano structure bone repair scaffold for freeze-drying delivery of exosomes overcomes the problem of limited osteogenesis induction performance of a pure MBG scaffold, improves the biological activity and osteogenesis promotion performance of the scaffold, and has an exosome slow release rate meeting the requirements of bone repair treatment, so that the bone repair ability and new bone growth ability of a scaffold material are improved, and complete regeneration / repair of bone tissues is realized. The microporous structure of the scaffold in the repair scaffold plays a role in protecting the form and activity of the exosome, and the multistage micro-nano structure bone repair scaffold for freeze-drying delivery of the exosome has a remarkably improved in-vivo bone defect repair effect.

A bionic bioactive 3D porous composite material for repairing bone is prepared through respectively grinding bioactive glass powder and pore forming agent, sieving, dissolving PHA in chloroform, adding said powder, constant-temp pressing, drying, treating in circulating deionized water, and immersing in physiological solution. Its advantages are high bone repairing power, high porosity (90%), excellent cell affinity, bioactivity and controllable biodegradability, and low cost.

The invention provides an exosome loaded icariin nano preparation, which is prepared by loading icariin on cow's milk exosomes, and the preparation method comprises the following steps: performing centrifuging for 30-60 minutes by using a centrifugal machine at 4 DEG C and 13,000-15,000 g, taking middle layer whey, and performing filtering by using a vacuum pump with 600-800 meshes; performing centrifuging at the temperature of 4 DEG C at 100000-150000 g for 1-2 hours, and taking the whey in the middle layer; and performing filtering by using a 1000-2000 mesh vacuum pump, performing centrifuging for 1-2 hours at the temperature of 4 DEG C under the condition of 13000-15000g, reserving 1-2 ml of whey at the bottom, collecting the whey in a 50ml EP tube, performing centrifuging for 1-2 hours at the temperature of 4 DEG C under the condition of 100000-150000g, discarding the supernatant, and separating out the milk exosome; mixing icariin and the exosome suspension at room temperature according to a ratio of 1: 9; and performing freezing and drying to obtain the exosome loaded icariin nano-drug. The material has the unique advantages that the availability is high, the cost is low, the toxicity is low, the yield is high, the medicine can be orally delivered, the large-scale production can be achieved, the bioavailability of the oral medicine can be potentially enhanced, the efficacy and the safety of the medicine can be improved, and the bone repair capability of the hostoside can be improved.

The invention relates to a composite hydrogel bracket, and a preparation method and application thereof. The preparation method of the above composite hydrogel bracket comprises the following steps of: mixing medical-grade sodium alginate, chondroitin sulfate and carboxymethyl chitosan with diluent to prepare mixed liquid; adding an aqueous solution containing water-soluble calcium salt into the mixed liquid, pouring into a mould, and standing for carrying out defoaming to prepare a hydrogel semi-finished product; and mixing the hydrogel semi-finished product with the aqueous solution of calcium carbonate, and preparing the composite hydrogel bracket. The preparation method of the above composite hydrogel bracket can prepare the composite hydrogel bracket which has a bone repairing effectand has high mechanical strength and good biocompatibility.

The invention provides a strontium-containing xenogeneic bone scaffold. The bone scaffold comprises a strontium-ion-loaded xenogeneic bone scaffold body and a polymer biological material surface coating. The good porosity characteristic of the xenogeneic bone scaffold body is used, firstly, strontium is adsorbed on the surface of the scaffold body, and then the surface of the scaffold body is coated with a polymer biological material to protect strontium. Strontium can be loaded in the xenogeneic bone scaffold body quickly, the residence time and release period of strontium on the scaffold body can also be prolonged, and it is hopeful to improve the bone repair effect of traditional xenogeneic bone scaffolds.

The invention discloses a bone hemostatic material. The bone hemostatic material is composed of poloxamer and a cellulose derivative. The bone hemostatic material in the invention has good adhesiveness, can effectively stop bleeding and promote healing of a bone injury part, can be degraded in the body, is free from immunogenicity and high in security, and can be ready to use.

The invention discloses a preparation method of a strontium-doped porous silk fibroin microsphere. The strontium-doped porous silk fibroin microsphere is prepared from silk fibroin and strontium chloride, wherein the porosity of the porous microsphere is 80 percent or above, the aperture is 5 to 40mu m, and the weight of the strontium chloride accounts for 5 to 38 percent of total weight. The silkfibroin disclosed by the invention is used as a natural protein module, is widely used for the health-care food industry and has no stimulating effect on a human body; strontium is a trace element which is essential for the human body, has the capabilities of osteogenic induction and the like, and can realize the effect of improving bone repair; meanwhile, the silk fibroin as a main material fora cell carrier can simulate protein components of an extracellular matrix. According to the strontium-doped porous silk fibroin microsphere prepared by the preparation method disclosed by the invention, slow release of strontium can be realized, and the problems that growth factors are obtained from animal body for use and the like are effectively solved.

A bionic bioactive 3D porous composite material for repairing bone is prepared through respectively grinding bioactive glass powder and pore forming agent, sieving, dissolving PHA in chloroform, adding said powder, constant-temp pressing, drying, treating in circulating deionized water, and immersing in physiological solution. Its advantages are high bone repairing power, high porosity (90%), excellent cell affinity, bioactivity and controllable biodegradability, and low cost.

The invention discloses a preparation method of an icariin controlled release chitosan / hydroxyapatite composite scaffold material. The method comprises the following steps: (1) preparing a raw material solution; and (2) stirring and mixing a chitosan solution with hydroxyapatite slurry evenly to obtain mixed slurry, dropwise adding a succinic acid icariin solution at the amount of keeping the final concentration of succinic acid icariin in the mixed slurry at 1*10<-5> to 1*10<-2> mol / L, adding a cross-linking agent, adjusting the pH to be 5.0-6.5, further stirring and reacting for 10-120 minutes, centrifuging reaction liquid and removing bubbles after reaction is ended, injecting into a mold, and standing for 10-240 minutes to obtain a gel product; freezing, drying, washing, and freezing and drying again. The composite scaffold material disclosed by the invention is capable of improving the bone repair performance of the scaffold material through proliferation and differentiation of slowly released Ica regulating cells.

The present invention provides a preparation method and application of a bioceramic scaffold with micro-nano structure on the surface of hollow microspheres, which solves the problem of how to realize the synergistic effect of drug-loaded microspheres and bone repair scaffolds in the prior art to obtain a scaffold-microsphere drug controlled release system The technical problem is to put the 3D printed bioceramic support and hexametaphosphate solution into the hydrothermal reaction kettle for hydrothermal reaction. After the hydrothermal reaction is completed, a bioceramic support with a micro-nano structure on the surface of hollow microspheres is obtained. The present invention also Disclosed is the application of a bioceramic support with a micro-nano structure on the surface of hollow microspheres, which can be widely used in the technical field of biomedical materials.

The invention discloses a high-strength mineralized hydrogel and applications of the high-strength mineralized hydrogel in bone repair. According to the present invention, N-acryloyl glycinamide and vinylphosphonic acid are used as raw materials and are co-polymerized in the presence of an initiator to prepare a hydrogel, calcium ions and phosphorus ions are deposited in situ in the gel to form hydroxyapatite, the mineralized hydrogel pipe has good tensile performance and good compressive performance due to the physical cross-linking effect of the hydrogen bond and the ion cross-linking effectof the hydroxyapatite and the gel matrix in the gel structure, and due to the presence of hydroxyapatite, the osseointegration and bone repair ability of the gel pipe is improved.

The invention provides a preparation method and application of a biological ceramic scaffold with a hollow microsphere surface micro-nano structure, and solves the technical problem of how to realize the synergistic effect of a drug-loaded microsphere and a bone repair scaffold to obtain a scaffold-microsphere drug controlled release system in the prior art. A 3D printing biological ceramic scaffold and a hexametaphosphate solution are put into a hydrothermal reaction kettle to be subjected to a hydrothermal reaction, and after the hydrothermal reaction is completed, the biological ceramic scaffold with the hollow microsphere surface micro-nano structure is obtained, and the invention further discloses application of the biological ceramic scaffold with the hollow microsphere surface micro-nano structure, and the biological ceramic scaffold can be widely applied to the technical field of biomedical materials.

The invention relates to the technical field of biomedical materials, in particular to a bone repair product containing a regenerative medical material and a preparation method thereof. The materialsinclude a powder containing a regenerative medical material, and a liquid; the powder comprises the regenerative medical material, calcium phosphate, pyrophosphate and phosphonate functionalized gelatin; and the liquid is an acidic buffer solution. The bone repair material provided by the invention contains the regenerative medical material with a large specific surface area, and has huge reactionactivity. The bone repair material contains a gelatin component which can be degraded to form micropores after being implanted into human tissues and is beneficial to promoting repair of bone defectparts. The bone repair material contains a phosphonate medicinal component which can be specifically combined with hydroxyphosphine limestone in sclerotin, inhibit osteoclast activity, and inhibit sclerotin absorption. The regenerative medical material with high biological activity, the gelatin capable of being degraded to form micropores and the phosphonate pharmaceutical ingredient have a synergistic effect, so that the effect of the bone repair material for treating bone defects and bone loss is remarkably improved.

The invention discloses a preparation method of a strontium-doped porous silk fibroin microsphere. The strontium-doped porous silk fibroin microsphere is prepared from silk fibroin and strontium chloride, wherein the porosity of the porous microsphere is 80 percent or above, the aperture is 5 to 40mu m, and the weight of the strontium chloride accounts for 5 to 38 percent of total weight. The silkfibroin disclosed by the invention is used as a natural protein module, is widely used for the health-care food industry and has no stimulating effect on a human body; strontium is a trace element which is essential for the human body, has the capabilities of osteogenic induction and the like, and can realize the effect of improving bone repair; meanwhile, the silk fibroin as a main material fora cell carrier can simulate protein components of an extracellular matrix. According to the strontium-doped porous silk fibroin microsphere prepared by the preparation method disclosed by the invention, slow release of strontium can be realized, and the problems that growth factors are obtained from animal body for use and the like are effectively solved.