A kind of scaffold material for bone repair and its preparation method and application

A scaffold material and bone repair technology, applied in the field of bioengineering, can solve the problem that the speed of bone repair cannot meet the needs of patients, and achieve the effect of promoting mineralization and bone repair process and promoting deposition.

Active Publication Date: 2021-12-17
NAT UNIV OF DEFENSE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a bone repair scaffold material and its preparation method and application, which are used to overcome the defects that the bone repair material in the prior art only contains one protein so that the bone repair speed cannot meet the needs of patients.

Method used

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  • A kind of scaffold material for bone repair and its preparation method and application
  • A kind of scaffold material for bone repair and its preparation method and application
  • A kind of scaffold material for bone repair and its preparation method and application

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preparation example Construction

[0041] The present invention also proposes a preparation method of bone repair scaffold material, the preparation method comprising:

[0042] S1: Add the pore-enlarging agent to the acidic solution of the structure-directing agent, add magnesium source, calcium source, silicon source and phosphorus source, stir at 40-50°C until a uniform mixed solution is formed, and adjust the pH value of the mixed solution to Alkaline (under alkaline conditions, the pore order of the obtained mesoporous material can be improved, and a certain stability can be maintained), at 90-110°C (at this temperature, the curled carbon chain at room temperature will straighten And expand the volume, causing the diameter of the micelles to increase, thereby increasing the pore size) standing still to obtain a precipitate (further reaction crystallization, forming a gel), washing the precipitate to a pH of 7 to 7.4 (the purpose of washing is to remove adsorbed or entrained Impurities and solvents, pH is us...

Embodiment 1

[0075] The present embodiment provides a method for preparing a scaffold material for bone repair, comprising:

[0076] 5g structure directing agent PEO 20 -PPO 70 -PEO 20 (P123) was completely dissolved in 300mL of water, and the pH value of the solution was adjusted to 1 with concentrated HCl, and 7g of pore-enlarging agent 1,3,5-mesitylene (TMB) was added, stirred for 4 hours until uniform, and then magnesium nitrate, calcium nitrate, 10mL tetraethyl orthosilicate (TEOS), 2.6g triethyl phosphate (TEP), wherein the molar ratio of magnesium to calcium in magnesium nitrate and calcium nitrate is (1:3), after stirring at 40°C for 24h, use concentrated Ammonia water was used to adjust the pH to 10, and after adding the solution into the reaction kettle, it was allowed to stand at 90° C. for 80 hours to obtain a precipitate. The resulting precipitate was centrifuged at 10,000 rpm, the supernatant was removed, the precipitate was washed several times with deionized water to pH ...

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Abstract

The invention discloses a bone repair scaffold material and its preparation method and application. The bone repair scaffold material has a fibrous scaffold structure, and the bone repair scaffold material is composed of magnesium-doped mesoporous bioactive glass loaded with OOB fusion protein; The OOB fusion protein is formed by linking OCN, OPN and BGN; the preparation method includes preparing magnesium-doped mesoporous bioactive glass, obtaining the OOB fusion protein, loading and electrospinning. The bone repair scaffold material provided by the present invention has excellent bone repair performance, and can provide long-term supply for the bone repair process; the preparation method provided by the present invention can successfully prepare a bone repair scaffold material comprising three proteins of OCN, OPN and BGN , the bone repair scaffold material can be applied to clinical bone defect repair.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a bone repair scaffold material and its preparation method and application. Background technique [0002] Mesoporous bioactive glass is a porous material with a pore size ranging from 2 to 50 nm. It mainly contains silicon, calcium and phosphorus elements. It has a regular pore structure, large specific surface area and pore volume, and can provide a place for cell adhesion and material exchange. , which facilitates cell proliferation. Second, the huge specific surface area is favorable for the mineralization of matrix nodules, thus possessing great potential in bone repair. Studies have found that bioactive glass has a significant promotion effect on osteoinductive activity, so it has attracted great attention in bone repair. [0003] The bone matrix that promotes osteogenesis is mainly organic components, specifically matrix proteins secreted by osteoblasts into the bo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00A61L27/10A61L27/12A61L27/22A61L27/56A61L27/50C12N15/62C12N15/70
CPCA61L27/10A61L27/12A61L27/227A61L27/56A61L27/50C07K14/47C12N15/70C07K2319/00C08L89/00
Inventor 王晓燕林嘉宇柳珑
Owner NAT UNIV OF DEFENSE TECH
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