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Preparation method of hydrogel, osteoblast containing hydrogel and preparation method of osteoblast containing hydrogel

An osteoblast and hydrogel technology, which is applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems of poor porosity and pore size of hydrogels, and is conducive to cell growth, shortening the treatment period, The effect of improving cell viability and bone repair effect

Inactive Publication Date: 2016-07-27
宁波国际材料基因工程研究院有限公司
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Problems solved by technology

[0004] In order to overcome the problems of poor porosity and pore size of current hydrogels, the present invention provides a preparation method of hydrogels, osteoblast-containing hydrogels and preparation methods thereof

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Embodiment Construction

[0021] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the accompanying drawings and implementation examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0022] see figure 1 , the first embodiment of the present invention provides a method for preparing a hydrogel, which includes the following steps:

[0023] Step S1, providing dialdehyde hyaluronic acid and RGD; Step S2, using high-throughput screening technology to obtain the required target ratio of dialdehyde hyaluronic acid and RGD; and

[0024] Step S3, cross-linking dialdehyde hyaluronic acid and RGD according to the target ratio obtained in step S2 to form a desired hydrogel with a three-dimensional porous structure.

[0025] In some preferred embodiments of the present in...

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Abstract

The invention provides a preparation method of hydrogel, osteoblast containing hydrogel and a preparation method of the osteoblast containing hydrogel.The preparation method of the hydrogel includes the following steps that S1, dialdehyde hyaluronic acid and RGD are provided; S2, the objective ratio needed by dialdehyde hyaluronic acid and RGD is obtained through the high-throughput screening technology; S3, dialdehyde hyaluronic acid and RGD in S2 are crosslinked to form the needed hydrogel with a three-dimensional porous structure.The concentrations needed by the dialdehyde hyaluronic acid water solution and the RGD water solution and the objective ratio for mixing of the dialdehyde hyaluronic acid water solution and the RGD water solution are rapidly obtained through the high-throughput screening technology, and therefore the hydrogel material with the needed hole diameter size and porosity can be obtained, the hydrogel is beneficial for cell growth, especially, osteoblast can obtain better growth, differentiation and propagation conditions in the hydrogel material, the cell survival rate is effectively increased accordingly, and the bone repair effect is effectively improved accordingly.

Description

【Technical field】 [0001] The invention relates to the technical field of biological tissue engineering, in particular to a preparation method of a hydrogel, an osteoblast-containing hydrogel and a preparation method thereof. 【Background technique】 [0002] Bone is one of the most important tissues in the human body, responsible for functions such as support, calcium storage, and hematopoiesis. As the scaffold of the human body, bone injury has always been a major problem that plagues human beings. At present, autologous bone transplantation, allogeneic bone transplantation and artificial bone transplantation are mainly used in the clinical treatment of bone injuries. However, the clinical application of these methods is limited due to the limited source and severe immune rejection. In recent years, due to its unique three-dimensional porous structure, the research on the use of hydrogel materials to treat bone injuries has attracted widespread attention, especially hydrogel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/38A61L27/52A61L27/56
CPCA61L27/26A61L27/3821A61L27/52A61L27/56A61L2430/02C08L5/08
Inventor 李楠楠向勇臧亮
Owner 宁波国际材料基因工程研究院有限公司
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