Method for using Pickering emulsion method for producing GelMA macropore hydrogel and application

A technology of hydrogel and emulsion method, applied in pharmaceutical formulation, medical science, prosthesis, etc., can solve the problems of unfavorable material biocompatibility, increase toxicity, unable to form connected pores, etc., to promote proliferation and osteogenic differentiation ability, enhanced delivery, good biocompatibility, and effects of degradability

Active Publication Date: 2019-07-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current methods commonly used to prepare biomedical macroporous scaffolds, either it is impossible to form interconnected pores, or additional chemicals need to be introduced to increase toxicity, which is not conducive to the biocompatibility of materials

Method used

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  • Method for using Pickering emulsion method for producing GelMA macropore hydrogel and application
  • Method for using Pickering emulsion method for producing GelMA macropore hydrogel and application
  • Method for using Pickering emulsion method for producing GelMA macropore hydrogel and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 (modification and characterization of gelatin)

[0036] 1. Modification of gelatin

[0037] GelMA is obtained by modifying gelatin with methacrylic anhydride. By grafting methacrylic anhydride to the gelatin molecular chain, a highly reactive carbon-carbon double bond is introduced into the gelatin molecular chain, which is beneficial to the four-armed PEG. The sulfhydryl groups on the -4SH molecule undergo a thiol-ene click reaction, and are cross-linked into a gel network under UV light. The gelation mechanism of GelMA macroporous hydrogel is shown in figure 1 .

[0038] Dissolve 5g of gelatin in 50mL of PBS (1×) buffer, in a 60°C water bath, stir until completely dissolved. 10 mL of methacrylic anhydride was dropped into the stirring gelatin solution at a rate of 0.5 mL / min, and reacted for 3 hours in a water bath at 50°C. After cooling slightly, the reaction was diluted to 5 times with 40°C PBS (1×) to terminate the reaction. Dialyze with a 12-14kD...

Embodiment 2

[0042] Embodiment 2 (contact angle test of MgO nanoparticles)

[0043] Pickering emulsion is an emulsion obtained by using ultrafine solid particles as an emulsifier. Assuming that the solid particles are spherical, the state of the particles in the oil-water phase, and the surface energy between particles-water, particle-oil, and water-oil are crucial to the type of emulsion formation. If the contact angle between the particle and the water phase θ90°, that is, the wettability of the particles and the oil phase is better, and the system is easy to form a water-in-oil (W / O) emulsion. Therefore, we can roughly judge the type of emulsion by the contact angle between the particles and water, and the hydrophilicity and hydrophobicity of the particles must be moderate, and stable emulsions cannot be obtained if they are too hydrophilic or too hydrophobic.

[0044] Disperse the MgO powder in absolute ethanol, ultrasonically for 30 minutes, fully disperse evenly, and then drop it on a...

Embodiment 3

[0046] Embodiment 3 (compression performance test of GelMA macroporous hydrogel scaffold)

[0047] Dissolve 0.05g GelMA in 0.25mL PBS solution (1×), stir until fully dissolved. Mix 0.25mL 0.2g / mL PEG-4SH (10,000 molecular weight) solution with GelMA solution evenly. Add 1.5mg of nano-MgO into 0.5mL of dodecane, and sonicate for 15min. Mix GelMA, PEG-4SH, dodecane, and I 2959 photoinitiator, and stir for 60s at 16,000 rpm with a homogenizer. Transfer the emulsion to a PDMS mold with a thickness of 5 mm and a diameter of 1 cm, and UV light for 60 seconds on the front and back sides. Finally, a GelMA macroporous hydrogel scaffold for compressive modulus testing was prepared. The compression curves of hydrogel scaffolds with 0.3wt% and 0.5wt% MgO content are shown in Figure 4 .

[0048] The 0.5wt% group has a higher MgO content and a smaller emulsion droplet size, so the pore size of the hydrogel is smaller, so the sample with a higher MgO content has a higher compressive mo...

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Abstract

The invention discloses a method for using a Pickering emulsion method for producing GelMA macropore hydrogel and application. The method comprises the steps of dissolving GelMA and PEG-4SH in PBS buffer liquid, and stirring a mixture; adding MgO nano-particles after fully dissolving the GelMA and the PEG-4SH, stirring a mixture, and conducting ultrasonic treatment on the mixture; adding dodecaneand a photoinitiator, and using a high-speed homogenizer for intensively stirring a mixture to obtain stable Pickering emulsion; and making the emulsion subjected to UV illumination to form gel, and obtaining the GelMA macropore hydrogel by using ethyl alcohol and water for full wash dialysis. The produced GelMA macropore hydrogel has the advantage of the macropore structure, can promote transportation of nutrient substances, discharging of metabolic products and cell communication, entraps a component containing Mg, has effect of promoting adhesion and proliferation of bone marrow mesenchymalstem cells and promoting osteogenic differentiation, has excellent biocompatibility, can be used as a bone tissue engineering scaffold with excellent performance, and has large clinical application potential in the field of bone repair.

Description

technical field [0001] The invention relates to the technical field of hydrogel materials, in particular to a method and application of GelMA macroporous hydrogel prepared by Pickering emulsion method. Background technique [0002] Bone injury is a common clinical surgical disease. The bone grafts currently used in clinical treatment mainly include autologous bone, allogeneic bone and xenograft bone. These three clinical bone grafts have their own advantages and disadvantages, and the sources are limited. Therefore, a large amount of bone repair materials are required for clinical treatment every year. Various bone repair materials used clinically also have certain problems. For example, medical metal materials have poor osteoconductivity and do not match the elastic modulus of natural bone tissue in the human body; calcium phosphate bone cement has poor mechanical strength and cannot be used for load bearing. Bone repair: The degradation rate of bioactive glass does not ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L89/00C08L71/02C08K3/22A61L27/50A61L27/52A61L27/54A61L27/02A61L27/22A61L27/18
CPCA61L27/025A61L27/18A61L27/222A61L27/50A61L27/52A61L27/54A61L2300/102A61L2300/412A61L2400/12A61L2430/02C08J3/075C08J2371/02C08J2389/00C08J2471/02C08K2003/222C08K2201/011C08L71/02
Inventor 曹晓东潘昊田李庆涛
Owner SOUTH CHINA UNIV OF TECH
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