High throughput oligonucleotide sequencing method

Abstract

The present invention relates to the field of gene engineering, provides a high throughout oligonucleotides sequencing method. Said sequencing method includes following steps: the generation of a short DNA tag, single-molecule PCR amplification benefit-marking sequence, enrichment of high-density DNA tags and large-scale cycle parallel sequencing, etc. By using a molecular scale for parallel sequencing in a sequencing method which takes complexation as the base or expanded synthesis as the base, the scale or an expanded anchor primer is used for expanding the base points which are read out on a template. By using the primer, longer sequence on the template can be read out, and the only identification can be obtained from the genome sequence of various organisms with the range from microorganisms, plants, and animals to human, thereby having greater practical value.

Description

field of invention [0001] The invention relates to a gene sequencing method, in particular to a high-throughput oligonucleotide sequencing method. Background technique [0002] As the number of genomes that have been sequenced increases, it is necessary to analyze a large amount of information encoded by genome sequences and the biological functions controlled and participated by these different sequence elements scattered in the genome. However, this is more complex than genome sequencing and requires powerful tools and systematic approaches that can handle processes with a large number of parameters and under different conditions, both normal and abnormal. Only such a comprehensive research method can make us understand most of the basic principles of biology. Of course, such research will also lead to major technological advances for the benefit of mankind. [0003] A major method developed in functional genomics research involves the quantitative analysis of DNA sequen...

AI Technical Summary

Problems solved by technology

These various ligase-based DNA sequencing methods can be used to perform parallel sequencing of monoclonal DNA arrays constructed by the methods described above, however, the cost of sequencing remains a huge barrier to the application of sequencing technology at the genome level

[0007] The problem with the existing technology is that while oligonucleotide arrays have contributed enormously to advances in functional genomics and systems biology, there are fundamental limitations to this technology

The cost is also very high, which seriously restricts the role of SAGE, and it is almost impossible to expand its application to clinical medicine.

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