DNA tags, PCR primer and application thereof

Abstract

The invention discloses DNA tags, a PCR primer and application thereof. The DNA tags of one group are chosen from nucleotides shown as in SEQ ID NO:1-95 and can be used for building a nucleic acid sequencing library to accurately differentiate the nucleic acid sequencing library. The DNA tags and the PCR primer are utilized to build a tagged PCR primer, and by utilizing the tagged PCR primer, STR detection of at most 95 DNA samples can be realized at one step according to a method for determining preset STR gene locus genotype of various DNA samples.

Description

technical field [0001] The present invention relates to the technical field of nucleic acid sequencing and typing, in particular, to DNA tags, PCR primers and applications thereof, more specifically, to a set of DNA tags, a set of PCR primers, a set of tagged PCR primers, and construction of nucleic acid sequencing libraries The method, the method for determining the genotype of the predetermined STR locus of a DNA sample, the kit for constructing a nucleic acid sequencing library and the system for determining the genotype of the predetermined STR locus of a DNA sample. Background technique [0002] STR locus sequence, also known as short tandem repeat sequence (Shorttandem repeat) is a kind of widely distributed genetic markers in the human genome, usually composed of 2-7 core bases, and the number of repeat units is different, resulting in different individuals in the same locus. alleles. The allele types can be typed by techniques such as silver staining, fluorescent la...

AI Technical Summary

Problems solved by technology

However, due to technical limitations, this method also has certain defects, mainly including: (1) Due to the mutual interference of fluorescent markers and the limitations of capillary length and imaging technology, the number of analyzed STR loci has been limited. It is difficult to further greatly improve; (2) Since the object of analysis is the length of each fragment, it is impossible to further detect the slight difference in the primary structure of the nucleic acid that makes up the fragment, thus limiting the resolution of detection; (3) There are invalid alleles , resulting in differences in the determination results of some loci that may occur in different kits; (4) the interference of Stutter peaks (small peaks that sometimes appear before the main peak in fragment analysis), especially when there are mixed samples; (5) Sanger method For STR typing, due to throughput, cost and other reasons, it is not conducive to the construction of STR typing database

[0004] Therefore, the current detection and typing methods of STR loci still need to be improved

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