31results about How to "High sequencing throughput" patented technology

The invention relates to the field of biochemical equipment and provides gene sequencing equipment, which comprises a sequencing reaction chamber and a temperature control component. The sequencing reaction chamber is used for fixing samples and performing sequencing reaction; the temperature control component is fixed in an area of the sequencing reaction chamber when the samples are fixed and used for controlling temperature of the sequencing reaction chamber. The temperature of the sequencing reaction chamber rises and decreases quickly by the temperature control component, and sequencing reaction efficiency is improved.

The invention relates to the field of genetic engineering, in particular to a gene sequencing device and a gene sequencing system. The gene sequencing device comprises a sample preparation component, a liquid conduction component, a sample loading component, a temperature control component, an image acquisition component and a control component. The gene sequencing system comprises a sample preparation control module, a liquid conduction control module, a reaction control module, a temperature control module, an image acquisition control module and a control module. By adopting the gene sequencing device and the gene sequencing system, the integration from sample preparation to data processing can be realized, and full automation of gene sequencing can also be realized. Meanwhile, the high-pass and low-cost gene sequencing can be realized.

The present invention provides a method and equipment for predicting neoantigen epitopes. The method for predicting neoantigen epitopes includes: (1) constructing a candidate epitope library, wherein the candidate epitope library is composed of differentially expressed peptides, and the expression differentially Peptides are peptides that differ between samples with the predetermined state and samples without the predetermined state; (2) building a predictive model based on a historical database composed of known antigenic epitope composition; and (3) using at least a subset of the candidate epitope library as an input variable of the prediction model, so as to predict new antigenic epitopes. Using this method of predicting neoantigen epitopes, it is possible to truly determine the mutation information of antigens from the gene level and protein level, and then quickly and effectively obtain neoantigen epitopes that can bind to MHC, and can accurately predict the relationship between antigen epitopes and NHC At the same time, the method is simple and convenient, and can greatly save manpower and material resources.

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and / or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

The invention discloses a magnetic bead enrichment technique based method for high-flux development of genome SSR (simple sequence repeat) markers. The method comprises the steps of: (1) extracting target genome DNA; (2) subjecting the obtained genome DNA to random fragmentation; (3) constructing a genome random fragmented DNA library; (4) amplifying the DNA library; (5) utilizing a biotin labeled SSR probe to perform hybridization with a target fragment in the library; (6) carrying out streptavidin magnetic bead enrichment on the SSR sequence-containing DNA library fragments; (7) amplifying and purifying the enriched DNA library fragments; (8) subjecting the purified DNA fragments to emulsion PCR amplification and sequencing; and (9) searching sequences containing SSR loci in the obtained sequences. The method reduces the library construction difficulty, shortens the experimental period, improves the sequencing flux, lowers the sequencing cost, and can be widely used as an effective method for high-flux development of various species SSR markers.

The invention relates to a method for linking monomolecular DNA to a single magnetic bead, belonging to the technical field of biology and comprising the following steps: performing fluorescence labeling to DNA, then labeling biotin at the end 3' of the DNA and labeling digoxin at the end 5' of the DNA; or labeling digoxin at the end 3' of the DNA and labeling biotin at the end 5' of the DNA; placing the DNA on glass to carry out an immunoreaction and washing the DNA by running water; carrying out the immunoreaction again; adding DNA endonuclease to the periphery of magnetic beads, after DNA molecules are degraded, collecting magnetic beads carrying DNA fragments one by one by a micro-gunpoint and placing the magnetic beads on a magnet; and cutting target DNA by the endonuclease and linking target DNA fragments with the DNA fragments on the magnetic beads under the action of ligase. The method for linking the monomolecular DNA to the single magnetic bead can acquire the sequence information of target fragments and realize the monomolecular sequencing of the DNA; and when nano-pores are produced into an array to be matched with a decoy array, sequencing throughput can be improved.