The invention discloses a gene sequencing method capable of increasing the signal-to-noise ratio by detecting pyrophosphoric acid charges. According to the method, by adding dNTP, polymerases and catalysts to a reaction system, pyrophosphoric acid is released through base pairing, pyrophosphoric acid is hydrolyzed to generate hydrogen phosphate ions, and the hydrogen phosphate ions are combined with sensor surface receptors to generate electrical signals which are further translated into corresponding bases. Pyrophosphoric acid generated during amplification can be detected, the diffusion rate of pyrophosphoric acid is much slower than that of hydrogen ions because the relative molecular mass of pyrophosphoric acid is 174, electrical signals which can be detected last for a long time, and accuracy can be effectively improved. Besides, pyrophosphoric acid has four positive charges, diffusion is slowed down, effective surface concentration is high, the converted electric signals are strong, and the detected signal-to-noise ratio is high. The high signal-to-noise ratio allows the use of smaller sensors and a sensor array with higher density and larger scale, so that sequencing throughput can be further improved and sequencing cost can be reduced.