Indexes for digital gene expression profiling (DGE) and use method thereof

A gene expression profiling and tag technology, applied in the fields of tags and their using methods, second-generation sequencing technology, and solexa sequencing technology, can solve the problem of low repeatability of experimental results, unreliable data results, and inability to truly reflect sample-related information, etc. problems, to achieve the effect of increasing sequencing throughput and reducing costs
CN102409044AActive Publication Date: 2012-04-11BGI TECH SOLUTIONS
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BGI TECH SOLUTIONS
Publication Date
2012-04-11

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Abstract

In the invention, a unique index sequence (index1-12) is designed aiming at a digital gene expression profiling (DGE) library sample building method based on a Solexa Single End sequencing platform provided by the current illumina company, and indexes are embedded in a 3' adapter in a DGE library by adapters, thus successfully establishing a DGE index library building method. The method is suitable for building the DGE index library of any eukaryote RNA sample and is successfully used for solexa sequencing, thus not only increasing the sequencing throughput of the DGE samples but also lowering the cost of solexa aiming at DGE sequencing.
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Description

technical field

[0001] The invention relates to the technical field of nucleic acid sequencing, in particular to the technical field of digital gene expression spectrum. In addition, the present invention also relates to a tag and a method for using it, and a method for constructing a digital gene expression spectrum library using the tag technology. The method of the present invention is particularly suitable for the second generation sequencing technology, especially the solexa sequencing technology. Background technique

[0002] Digital Gene Expression Profiling (DGE) uses next-generation high-throughput sequencing technology and high-performance computing analysis technology to comprehensively, economically and quickly detect the gene expression of a specific tissue of a species in a specific state. Digital gene expression profiling has been widely used in basic scientific research, medical research and drug development and other fields.

[0003] The specific tags of m...

Claims

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