DNA(deoxyribonucleic acid) index library building method based on PCR (polymerase chain reaction)

A construction method and labeling technology, which is applied in the field of DNA library construction, can solve the problems of low PCR amplification efficiency, small number of labels, and high cost, and achieve the effects of increasing sequencing throughput, improving efficiency, and reducing sequencing costs
CN102409049AActive Publication Date: 2012-04-11BGI TECH SOLUTIONS
3 Cites 25 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BGI TECH SOLUTIONS
Publication Date
2012-04-11

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention designs 161 unique index sequences with a length of 8bp, and the indexes are embedded into DNA PCR (deoxyribonucleic acid polymerase chain reaction) primers to form DNA PCR index primers, thus being capable of importing the index sequences through PCR. The invention successfully establishes a method for building a DNA index library, and the method is applied to solexa DNA sequencing.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field

[0001] The invention relates to the technical field of DNA library construction, in particular to the technical field of DNA tag library construction, in particular to a PCR-based DNA tag library construction method. In addition, the present invention also relates to labeling technology and a method for implementing library construction of multiple samples in the same reaction system. The method of the present invention is particularly suitable for second-generation sequencing technology, especially solexa sequencing technology. Background technique

[0002] The Solexa DNA sequencing platform provided by Illumina can add four fluorescently-labeled nucleotides to one reaction at the same time, using Sequencing By Synthesis (SBS), which has a small sample volume, high throughput, and high throughput. Accuracy, with a simple and easy-to-operate automation platform and powerful functions [1-4]. The library construction first needs to repair the end of the target fra...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More