Sebastiscus marmoratus gene screening and mining method based on simplified genome sequencing technology

Abstract

The invention provides a sebastiscus marmoratus gene screening and mining method based on a simplified genome sequencing technology, and belongs to the technical field of molecular marker development. The method comprises steps as follows: genome DNA extraction; genome DNA double digestion; linkage connection; PCR (polymerase chain reaction) amplification; sequence fragment recovery and high-throughput sequencing; data analysis and screening and gene locus mining. According to the method, extracted DNA has higher quality, enzyme cleavage rate is effectively increased, primer design difficulty is reduced, recovered fragments have more uniform size, the sequencing amount is larger, and a sequencing result is more accurate; areas such as a high-repetition area and the like with relatively higher methylation degree can be avoided, so that enzyme cleavage fragments with more specific sequences are collected, and marking effectiveness of SNPs (single nucleotide polymorphisms) for following identification is higher; obtained nucleic acid molecules have quite low preference and high complexity, and therefore, the maximum gene coverage is obtained with the least sequencing data during sequencing.

Description

technical field [0001] The invention belongs to the technical field of molecular marker development, and in particular relates to a method for screening and excavating genes of scorpionfish based on simplified genome sequencing technology. Background technique [0002] The brown calamari belongs to the order Scorpioformes, Scorpidae, and genus Calamus, commonly known as stone dog male, tiger head fish, etc. It is a kind of offshore oviparous marine fish. The brown calamari is mainly distributed in the Northwest Pacific Ocean from Japan to the coastal waters of the Philippines. It is a rocky and bottom-dwelling fish. Its meat is delicious and has high nutritional value. It is an important offshore economic fish and a potential excellent species for artificial breeding and multiplication and release. At present, the research on the brown calamari mainly focuses on the research of physiology, biochemistry, biology and genetics. Among them, the biological research of the brown...

AI Technical Summary

Problems solved by technology

At present, there are no reports on the genetics and breeding of the brown calamari. Considering the important economic value and ecological value of the brown calamari, it is of great significance to carry out the genetic breeding and artificial breeding of the brown calamari for the management and protection of the germplasm resources of the brown calamari.

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