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Nucleic acid detection reaction platform and nucleic acid detection system

A detection system and reaction platform technology, applied in bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, biochemical instruments, etc., can solve the problem of low sequencing throughput, and achieve improved sequencing throughput, The effect of high-throughput sequencing

Active Publication Date: 2013-05-08
盛司潼
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Problems solved by technology

[0006] The purpose of the present invention is to provide a nucleic acid detection reaction platform and a nucleic acid detection system, aiming to solve the problem of low sequencing throughput in the prior art

Method used

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  • Nucleic acid detection reaction platform and nucleic acid detection system
  • Nucleic acid detection reaction platform and nucleic acid detection system
  • Nucleic acid detection reaction platform and nucleic acid detection system

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no. 4 example

[0063] Based on the fourth embodiment, the present invention proposes a fifth embodiment. Such as Figure 10 As shown, the sequencing reaction chamber 3 includes a sequencing reaction chamber, a reagent inlet 31 and a reagent outlet 32 ​​.

[0064] (1) The sequencing reaction chamber is used for sequencing reactions.

[0065] (2) The reagent inlet 31 is used to receive reagents.

[0066] (3) The reagent outlet 32 ​​is used to discharge the reagents in the sequencing reaction chamber.

[0067] The sequencing reaction chamber 3 may be a sequencing reaction chamber 3 including multiple sequencing reaction chambers, or may be a sequencing reaction chamber 3 including a single sequencing reaction chamber. Preferably, the sequencing reaction chamber 3 includes 1-16 sequencing reaction chambers. Such as Figure 10 As shown, the sequencing reaction chamber 3 is located on the base plate 4, and the top plate 5 is respectively opened at the positions corresponding to the reagent ou...

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Abstract

The invention relates to the field of biochemical equipment, and provides a nucleic acid detection reaction platform. The nucleic acid detection reaction platform comprises a temperature control component, a control component and at least two sequencing reaction chambers, wherein the control component is used for adjusting set temperature to target temperature and controlling a sequencing reaction to reach the target temperature from the current temperature and further used for sending a target temperature instruction to the temperature control component; the temperature control component is used for heating or cooling the sequencing reaction according to the target temperature instruction of the control component, and measuring and feeding back the current temperature of the sequencing reaction in real time; and the sequencing reaction chamber is used for parallel sequencing. The invention further provides a nucleic acid detection system containing the nucleic acid detection reaction platform. The nucleic acid detection reaction platform and the nucleic acid detection system related by the invention can improve the quality of the sequencing reaction and further can improve the sequencing throughput.

Description

technical field [0001] The invention relates to the field of biochemical equipment, and more specifically, to a nucleic acid detection reaction platform and a nucleic acid detection system. Background technique [0002] Since the implementation of the Human Genome Project, gene sequencing technology has developed very rapidly. The original manual sequencing has been replaced by semi-automatic instrument operation, and fully automated control will be the mainstream of future development. In terms of sequencing efficiency and cost, the progress from the first human genome sequencing, which took 13 years and cost US$3.5 billion, to the second human genome, which took only one year and cost US$70 million, is remarkable. These remarkable advances are not only related to the improvement of sequencing methods, but also inseparable from the performance improvement of various sequencing devices. [0003] Although the sequencing throughput and sequencing cost of the current sequencin...

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Application Information

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IPC IPC(8): C12M1/38C12M1/36C12M1/34
Inventor 盛司潼
Owner 盛司潼
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