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Application of Remdesivir in preparation of anti-bovine parainfluenza virus 3 medicine

A technology of remdesivir and drugs, which is applied in the field of application of remdesivir in the preparation of anti-bovine parainfluenza virus type 3 drugs, and can solve problems such as prevention or treatment of bovine parainfluenza virus type 3.

Pending Publication Date: 2021-05-28
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no report about its use in the prevention or treatment of bovine parainfluenza virus type 3

Method used

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  • Application of Remdesivir in preparation of anti-bovine parainfluenza virus 3 medicine
  • Application of Remdesivir in preparation of anti-bovine parainfluenza virus 3 medicine
  • Application of Remdesivir in preparation of anti-bovine parainfluenza virus 3 medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] virus-TCID 50 Determination of

[0055] MDBK cells (preserved by Dairy Cow Research Center, Shandong Academy of Agricultural Sciences) were digested and mixed with 1 × 10 per well. 5 Cells / mL were seeded into 96-well cell culture plates, placed in 37°C, 5% CO 2 After being cultured into a single layer of cells in a cell culture box, the cell growth solution in the well was discarded, and the virus dilution solution of BPIV3 serial 10-fold dilution (dilutions were 10 -1 ~10 -10 ) inoculated in a 96-well plate full of monolayer cells, 100 μL per well, placed in 37°C, 5% CO 2 Continue to culture in the incubator, observe the CPE of the cells day by day, and record the number of cells with pathological changes in detail. At the same time, a normal cell control group and a blank control group were set up, with 8 replicates in each group, and the results were judged when no further cell lesions occurred. The cell pathological well is the cell hole corresponding to the ab...

Embodiment 2

[0066] Toxicity test of Remdesivir on MDBK cells:

[0067] MDBK cells are susceptible to BPIV3. Therefore, the cytotoxicity of Remdesivir to MDBK cells was first detected, and the specific experimental steps were as follows:

[0068] (1) Inoculate 100 μL cells (MDBK 1×10 4 pieces / hole).

[0069] (2) After cultured to the MDBK monolayer, the next step of drug addition analysis was performed. Discard the medium, add 100 μL of 2% FBS DMEM containing different drug concentrations to each well, and make 3 parallels for each concentration. At the same time as the control well: add 100 μL of 2% FBS DMEM medium. Zero well: no cells are plated.

[0070] (3) At 37°C, 5% CO 2 After culturing under the conditions for 48 hours, operate according to the instructions of the CCK-8 kit, and measure the OD value at 450nm with a microplate reader.

[0071] (4) 37°C, 5% CO 2 After culturing for 2 h under the same conditions, the absorbance was measured at 450 nm. A450 nm of normal growin...

Embodiment 3

[0075] Inhibition experiment of Remdesivir on BPIV3:

[0076] (1) Inoculate 1×10 in each well of a 96-well plate 4 MDBK cells, 37°C, 5% CO 2 Cultivate overnight in an incubator;

[0077] (2) Discard the medium and add 100 μL 1000TCID to each well 50 The BPIV3 dilution solution (use 2% FBS DMEM to add the virus dilution solution after the cells are overgrown, according to the initial concentration of 25μM, double the concentration gradient dilution and dosing, 5% CO 2 Cultivated in an incubator;

[0078] (3) After 48 hours, operate according to the instructions of the CCK-8 kit, and measure the OD value at 450 nm with a microplate reader.

[0079] (4) Analyze data, virus inhibition rate (%)=(drug treatment group D450nm value-virus control group D450 nm value) / (normal cell control group D450 nm value-virus control group D450nm value) * 100%, use GraphPad Prism5 The half effective concentration (EC) of the compound obtained by the software 50 )value. The result is as ima...

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Abstract

The invention relates to the technical field of medicines, and particularly relates to application of Remdesivir in preparation of an anti-bovine parainfluenza virus 3 medicine. The anti-BPIV3 comprises one or more of the following effects: proliferation of the BPIV3 virus is inhibited; the BPIV3 virus is inactivated; the adsorption effect of BPIV3 on cells is prevented; and the replication of the BPIV3 in cells is blocked. According to the application, it is found for the first time that the compound, namely, the Remdesivir, can effectively inhibit proliferation of the BPIV3 and is small in toxicity to cells, experiments prove that the Remdesivir can effectively inhibit and kill the BPIV3 on an in-vitro experiment MDBK cell model, invasion and replication of the BPIV3 can be effectively inhibited, the Remdesivir can be used as a novel anti-BPIV3 medicine, a novel medicine application is developed for the Remdesivir, an experimental foundation is laid for developing efficient and specific anti-BPIV3 medicines, and a new visual field is provided.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of remdesivir in the preparation of anti-bovine parainfluenza virus type 3 drugs. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Bovine parainfluenza virus type 3 (BPIV3) belongs to the family Paramyxoviridae and the genus Respirovirus, and is the most important pathogen of bovine respiratory syndrome (Bovine respiratory disease complex, BRDC). , wheezing, loss of appetite, increased eye and nose secretions, a few accompanied by diarrhea, and finally pneumonia, which can cause miscarriage in severe cases. At present, BPIV3 infection has been distributed worl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/685A61K45/06A61P31/14
CPCA61K31/685A61K45/06A61P31/14
Inventor 程凯慧于志君杨宏军楚会萌朱彤任亚初
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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