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Primers, probes and kit for real-time fluorescent quantitative PCR detection of bovine epidemic diseases

A technology of real-time fluorescence quantification and detection kits, which is applied in the fields of biochemical equipment and methods, microbial measurement/inspection, microorganisms, etc., to achieve the effect of rapid detection

Inactive Publication Date: 2021-08-13
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Have not yet seen the reagent kit that adopts fluorescent quantitative PCR method to simultaneously detect multiple cattle blight pathogens with Tagman probe

Method used

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  • Primers, probes and kit for real-time fluorescent quantitative PCR detection of bovine epidemic diseases
  • Primers, probes and kit for real-time fluorescent quantitative PCR detection of bovine epidemic diseases
  • Primers, probes and kit for real-time fluorescent quantitative PCR detection of bovine epidemic diseases

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Embodiment Construction

[0041] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. The specific embodiments described here are only used to explain the present invention, not to limit the invention.

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Abstract

The invention discloses primers, probes and a kit for real-time fluorescent quantitative PCR (polymerase chain reaction) detection of bovine epidemic diseases. The kit at least comprises primers and probes for detecting BHV (Bovine herpesvirus), BPIV (bovine parainfluenza virus), SC type MmmSC (Mycoplasma mycoides subsp mycoides SC), BRSV (Bovine respiratory syncytial virus), BTV (bluetongue virus), BVDV (bovine viral diarrhea virus), BRV (bovine rotavirus), PPRV (peste des petits ruminants virus), RPV (Rinderpestvirus), VSV (vesicular stomatitis virus), FMDV (foot-and-mouth disease virus) and IC. Compared with the prior art, the four TaqMan multiple real-time fluorescent quantitative PCR systems of BHV, BPIV, MmmSC and IC, BRSV, BVDV, BTV and IC, BRV, PPRV, RPV and IC, FMDV, VSV and IC are constructed, and the specificity, repeatability and sensitivity of the systems all reach expected effects, so that the 11 pathogens can be rapidly detected at the same time.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to PCR detection technology for bovine blight pathogens, in particular to primers, probes and kits for real-time fluorescent quantitative PCR detection of bovine blight. Background technique [0002] In recent years, my country's cattle breeding industry has developed rapidly, and the current cattle stock and milk production have ranked third in the world. However, compared with developed countries, the development foundation of my country's cattle breeding industry is still weak, and it is facing many urgent problems and problems. Severe challenges, especially the prevention and control of infectious diseases. Due to the rapid development of large-scale and intensive breeding, as well as the frequent transfer of a large number of imported cattle from abroad and domestic live cattle, the infectious diseases occurring in my country's cattle industry have become more and more comple...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/689C12Q1/6851C12N15/11C12R1/93C12R1/35
CPCC12Q1/705C12Q1/701C12Q1/689C12Q1/6851C12Q2600/16C12Q2531/113C12Q2545/114C12Q2561/101C12Q2537/143
Inventor 王升启刘琪琦韦佳塔侯广争张影陈海兰
Owner ACADEMY OF MILITARY MEDICAL SCI
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