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Application of sulfamethoxypyrazine in preparation of product for preventing and/or treating bovine parainfluenza virus

A technology of sulfamethoxazine and bovine parainfluenza virus, which is applied in the fields of cattle feed additives and bovine respiratory syndrome treatment drugs, can solve the problems of severe, decreased respiratory mucosal defense ability, increased mortality and other problems, and achieves the prevention of proliferation. Effect

Active Publication Date: 2021-08-27
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease is common in late autumn and winter. Cattle infected with BPIV3 are often prone to secondary infection of severe bacterial or Mycoplasma disease, which causes severe pneumonia and greatly increases the mortality rate

Method used

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  • Application of sulfamethoxypyrazine in preparation of product for preventing and/or treating bovine parainfluenza virus
  • Application of sulfamethoxypyrazine in preparation of product for preventing and/or treating bovine parainfluenza virus
  • Application of sulfamethoxypyrazine in preparation of product for preventing and/or treating bovine parainfluenza virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Virus TCID 50 Determination of

[0042] MDBK cells (preserved by Dairy Cow Research Center, Shandong Academy of Agricultural Sciences) were digested and mixed with 1 × 10 per well. 5 Cells / mL were seeded into 96-well cell culture plates, placed in 37°C, 5% CO 2 After being cultured into a single layer of cells in a cell culture box, the cell growth solution in the well was discarded, and the virus dilution solution of BPIV3 serial 10-fold dilution (dilutions were 10 -1 ~10 -10 ) inoculated in a 96-well plate full of monolayer cells, 100 μL per well, placed in 37°C, 5% CO 2 Continue to culture in the incubator, observe the CPE of the cells day by day, and record the number of cells with pathological changes in detail. At the same time, a normal cell control group and a blank control group were set up, with 8 replicates in each group, and the results were judged when no further cell lesions occurred. The cell pathological well is the cell hole corresponding...

Embodiment 2

[0052] The toxicity experiment of embodiment 2 sulfamethoxine to MDBK cell

[0053] MDBK cells are susceptible to BPIV3. Therefore, the cytotoxicity of sulfamethoxine to MDBK cells was first detected, and the specific experimental steps were as follows:

[0054] (1) Inoculate 100 μL cells (MDBK 1×10 4 pieces / hole).

[0055] (2) After cultured to the MDBK monolayer, the next step of drug addition analysis was performed. Discard the medium, add 100 μL of 2% FBS DMEM containing different drug concentrations to each well, and make 3 parallels for each concentration. At the same time, control wells: add 100 μL 2% FBS DMEM medium. Zero well: no cells are plated.

[0056] (3) At 37°C, 5% CO 2 After culturing under the conditions for 48 hours, operate according to the instructions of the CCK-8 kit, and measure the OD value at 450nm with a microplate reader.

[0057] (4) 37°C, 5% CO 2 After culturing for 2 h under the same conditions, the absorbance was measured at 450 nm. A45...

Embodiment 3

[0060] Embodiment 3 Sulfamethoxine is to the inhibition experiment of BPIV3

[0061] (1) Inoculate 1×10 in each well of a 96-well plate 4 MDBK cells, 37°C, 5% CO 2 Cultivate overnight in an incubator;

[0062] (2) Discard the medium, add 100 μL of 2% DMEM to each well, and add the medicine according to the initial concentration of 50 μM, two-fold concentration gradient dilution, 5% CO 2 Culture in an incubator; after drug incubation for 2 hours, add 100 μL of 1000 TCID to each well 50 BPIV3 dilutions.

[0063] (3) After 48 hours, operate according to the instructions of the CCK-8 kit, and measure the OD value at 450 nm with a microplate reader.

[0064] (4) analysis data, virus inhibition rate (%)=(drug treatment group D450nm value-virus control group D450nm value) / (normal cell control group D450nm value-virus control group D450nm value) * 100%, get with GraphPad Prism5 software The half effective concentration of the compound (EC 50 )value. The result is as image 3 s...

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Abstract

The invention particularly relates to application of sulfamethoxypyrazine in preparation of a product for preventing and / or treating bovine parainfluenza virus. Bovine parainfluenza virus type 3 is an important pathogen causing cattle respiratory syndrome in a livestock farm, and the transmission route comprises respiratory tract and reproductive organ transmission which may cause serious pneumonia and even infertility. The research result provides the application of sulfamethoxypyrazine in development of products for preventing and / or treating bovine parainfluenza virus, and verification shows that in a safe dosage range of normal cells, sulfamethoxypyrazine shows an inhibition effect on bovine parainfluenza virus type 3, blocks an adsorption effect of the virus on the normal cells, and can be used for preventing and / or treating the bovine parainfluenza virus type 3. Sulfamethoxypyrazine is expected to be applied to development of veterinary drugs or feed additives.

Description

technical field [0001] The invention belongs to the technical field of veterinary drugs, and in particular relates to the application of sulfamethoxine in the preparation of products for the prevention and / or treatment of bovine parainfluenza virus, a pharmaceutical composition containing sulfamethoxine, a drug for treating bovine respiratory syndrome, Treatment methods and feed additives for cattle. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Bovine parainfluenza virus type 3 (BPIV3) belongs to the family Paramyxoviridae and the genus Respirovirus, and is the most important pathogen of bovine respiratory syndrome (Bovine respiratory disease complex, BRDC). , wheezin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/635A61K45/06A61P31/16A61P11/00A23K20/137A23K50/10
CPCA61K31/635A61K45/06A61P31/16A61P11/00A23K20/137A23K50/10
Inventor 程凯慧于志君楚会萌杨宏军任亚初
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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