Colloidal gold test strip based on goat pox virus and preparation method thereof

Abstract

The invention relates to a colloidal gold test strip based on a goat pox virus. The colloidal gold test strip comprises a PVC (Polyvinyl Chloride) rubber plate, a nitrocellulose membrane, a colloidal gold mat, a sample mat and absorbent paper, wherein the sample mat adheres to one end of the PVC rubber plate; the absorbent paper adheres to the other end of the PVC rubber plate; the colloidal gold mat and nitrocellulose membrane adhere to the middle part of the PVC rubber plate in sequence; the sample mat adheres to one end of the golden standard mat; the nitrocellulose membrane adheres to the other end of the colloidal gold mat; the nitrocellulose membrane adheres to the absorbent paper; the colloidal gold mat is coated with an anti-GTPV-P32 protein monoclonal antibody marked with colloidal gold; the nitrocellulose membrane is provided with a detection line which is linearly coated with an anti-GTPV-ORF122 protein monoclonal antibody at the concentration of 1mg / mL, and a quality control line which is linearly coated with a goat anti-mouse IgG (Intravenous Gamma Globulin) antibody at the concentration of 1mg / mL in sequence along the flow direction of a sample. Meanwhile, the invention further discloses a preparation method of the diagnosis strip. The test strip has the characteristics of high specificity, high stability, easiness in operation and rapid detection, and is suitable for field detection at the occurrence of animal epidemic diseases.

Description

technical field [0001] The invention relates to the technical field of virus detection, in particular to a gold standard detection test strip based on goat pox virus and a preparation method thereof. Background technique [0002] Goat pox virus (GTPV) is the pathogen of goat pox (Goat Pox, GTP), which mainly harms goats and sheep. The diseased sheep are characterized by fever, pox rash on the skin and mucous membranes, respiratory and digestive tract damage, and enlarged lymph nodes. Symptoms are an acute, febrile, contact infectious disease. The disease is endemic and is prevalent in my country and other countries in the world. When it breaks out or becomes popular, it will cause huge losses to the local sheep industry and economy. [0003] The genome size of goat poxvirus is 143-147 kb. The P32 gene is located at 64~65kb of the GTPV genome. P32 has antigen specificity. P32 contains an antigenic determinant, an envelope protein consisting of 319-324 amino acids, and a tr...

AI Technical Summary

Problems solved by technology

The AGP method is the most commonly used detection method at present. It is simple to operate, but it takes a long time, the result error is large, and the sensitivity is low. It is not suitable for early diagnosis of disease.

Immunofluorescence detection method, through the combination of fluorescein-labeled secondary antibody, the signal is amplified, and the detection sensitivity is improved to a certain extent through fluorescence microscope observation and identification. However, the fluorescent antibodies commonly used in immunofluorescence method are not stable enough, and the judgment is subjective. strong, prone to cross-reactions, and requires special equipment such as fluorescence microscopes, and is subject to many conditions in application

The neutralization test method is cumbersome to operate and has high accuracy, but it takes a long time, requires professionals to operate, and requires a lot of manpower

The PCR method is to design the primers of the conserved sequence on the GTPV genome, and then amplify it by PCR for detection. This method has high detection sensitivity and reliable results, but the determination method is cumbersome, time-consuming, low in efficiency, high in cost, and is interfered by other components in the sample. , requires special testing equipment, and operators need professional training, which is difficult to popularize

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